Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2386 and Shewana3_2387 are separated by 199 nucleotides Shewana3_2387 and Shewana3_2388 are separated by 21 nucleotides Shewana3_2388 and Shewana3_2389 are separated by 130 nucleotides
Shewana3_2386: Shewana3_2386 - long-chain-fatty-acid--CoA ligase (RefSeq), at 2,864,912 to 2,866,585
_2386
Shewana3_2387: Shewana3_2387 - alpha/beta hydrolase fold (RefSeq), at 2,866,785 to 2,867,651
_2387
Shewana3_2388: Shewana3_2388 - hypothetical protein (RefSeq), at 2,867,673 to 2,868,485
_2388
Shewana3_2389: Shewana3_2389 - hypothetical protein (RefSeq), at 2,868,616 to 2,869,323
_2389
Position (kb)
2866
2867
2868 Strain fitness (log2 ratio)
-1
0
1
2 at 2865.875 kb on - strand, within Shewana3_2386 at 2866.198 kb on - strand, within Shewana3_2386 at 2866.268 kb on - strand, within Shewana3_2386 at 2866.849 kb on - strand at 2866.865 kb on + strand at 2866.873 kb on - strand, within Shewana3_2387 at 2867.309 kb on - strand, within Shewana3_2387 at 2867.604 kb on + strand at 2867.617 kb on - strand at 2867.751 kb on + strand at 2867.833 kb on - strand, within Shewana3_2388 at 2868.222 kb on + strand, within Shewana3_2388 at 2868.400 kb on + strand, within Shewana3_2388 at 2868.426 kb on + strand at 2868.434 kb on - strand at 2868.434 kb on - strand at 2868.434 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 2,865,875 - Shewana3_2386 0.58 +0.7 2,866,198 - Shewana3_2386 0.77 +1.4 2,866,268 - Shewana3_2386 0.81 +0.5 2,866,849 - +0.1 2,866,865 + +0.9 2,866,873 - Shewana3_2387 0.10 -0.1 2,867,309 - Shewana3_2387 0.60 +1.5 2,867,604 + +1.3 2,867,617 - +1.5 2,867,751 + -0.9 2,867,833 - Shewana3_2388 0.20 +0.5 2,868,222 + Shewana3_2388 0.68 -1.2 2,868,400 + Shewana3_2388 0.89 +1.5 2,868,426 + +2.5 2,868,434 - -0.5 2,868,434 - +0.4 2,868,434 - -0.2
Or see this region's nucleotide sequence