Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2365 and rnhA are separated by 70 nucleotides rnhA and Shewana3_2367 are separated by 36 nucleotides Shewana3_2367 and Shewana3_2368 are separated by 23 nucleotides Shewana3_2368 and Shewana3_2369 are separated by 175 nucleotides
Shewana3_2365: Shewana3_2365 - DNA polymerase III, epsilon subunit (RefSeq), at 2,844,011 to 2,844,739
_2365
Shewana3_2366: rnhA - ribonuclease H (RefSeq), at 2,844,810 to 2,845,286
rnhA
Shewana3_2367: Shewana3_2367 - LysR family transcriptional regulator (RefSeq), at 2,845,323 to 2,846,222
_2367
Shewana3_2368: Shewana3_2368 - methyltransferase type 11 (RefSeq), at 2,846,246 to 2,846,980
_2368
Shewana3_2369: Shewana3_2369 - beta-lactamase domain-containing protein (RefSeq), at 2,847,156 to 2,847,959
_2369
Position (kb)
2845
2846
2847 Strain fitness (log2 ratio)
-1
0
1
2 at 2844.870 kb on - strand, within rnhA at 2845.080 kb on + strand, within rnhA at 2845.433 kb on - strand, within Shewana3_2367 at 2845.438 kb on - strand, within Shewana3_2367 at 2845.471 kb on - strand, within Shewana3_2367 at 2845.506 kb on + strand, within Shewana3_2367 at 2846.374 kb on + strand, within Shewana3_2368 at 2846.374 kb on + strand, within Shewana3_2368 at 2846.382 kb on - strand, within Shewana3_2368 at 2846.413 kb on - strand, within Shewana3_2368 at 2846.441 kb on - strand, within Shewana3_2368 at 2846.557 kb on - strand, within Shewana3_2368 at 2846.718 kb on - strand, within Shewana3_2368 at 2846.776 kb on + strand, within Shewana3_2368 at 2846.779 kb on - strand, within Shewana3_2368 at 2846.786 kb on + strand, within Shewana3_2368 at 2846.880 kb on - strand, within Shewana3_2368 at 2847.151 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 2,844,870 - rnhA Shewana3_2366 0.13 -0.7 2,845,080 + rnhA Shewana3_2366 0.57 -0.5 2,845,433 - Shewana3_2367 0.12 +0.4 2,845,438 - Shewana3_2367 0.13 +0.1 2,845,471 - Shewana3_2367 0.16 -0.4 2,845,506 + Shewana3_2367 0.20 +0.5 2,846,374 + Shewana3_2368 0.17 +0.1 2,846,374 + Shewana3_2368 0.17 +2.5 2,846,382 - Shewana3_2368 0.19 +0.7 2,846,413 - Shewana3_2368 0.23 -1.1 2,846,441 - Shewana3_2368 0.27 +1.5 2,846,557 - Shewana3_2368 0.42 -1.1 2,846,718 - Shewana3_2368 0.64 -1.7 2,846,776 + Shewana3_2368 0.72 -1.6 2,846,779 - Shewana3_2368 0.73 +0.3 2,846,786 + Shewana3_2368 0.73 -0.1 2,846,880 - Shewana3_2368 0.86 -0.3 2,847,151 + -0.5
Or see this region's nucleotide sequence