Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2157 and Shewana3_2158 are separated by 132 nucleotides Shewana3_2158 and Shewana3_2159 are separated by 27 nucleotides Shewana3_2159 and Shewana3_2160 are separated by 79 nucleotides
Shewana3_2157: Shewana3_2157 - Smr protein/MutS2 (RefSeq), at 2,586,084 to 2,586,674
_2157
Shewana3_2158: Shewana3_2158 - hypothetical protein (RefSeq), at 2,586,807 to 2,587,385
_2158
Shewana3_2159: Shewana3_2159 - SecC motif-containing protein (RefSeq), at 2,587,413 to 2,587,907
_2159
Shewana3_2160: Shewana3_2160 - diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq), at 2,587,987 to 2,590,170
_2160
Position (kb)
2587
2588 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2586.418 kb on + strand, within Shewana3_2157 at 2586.613 kb on + strand, within Shewana3_2157 at 2587.516 kb on + strand, within Shewana3_2159 at 2587.615 kb on + strand, within Shewana3_2159 at 2587.623 kb on - strand, within Shewana3_2159 at 2587.623 kb on - strand, within Shewana3_2159 at 2587.635 kb on + strand, within Shewana3_2159 at 2587.651 kb on + strand, within Shewana3_2159 at 2587.681 kb on + strand, within Shewana3_2159 at 2587.895 kb on + strand at 2588.416 kb on - strand, within Shewana3_2160 at 2588.416 kb on - strand, within Shewana3_2160 at 2588.798 kb on + strand, within Shewana3_2160
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 2,586,418 + Shewana3_2157 0.57 +2.5 2,586,613 + Shewana3_2157 0.90 +2.5 2,587,516 + Shewana3_2159 0.21 -0.1 2,587,615 + Shewana3_2159 0.41 -0.1 2,587,623 - Shewana3_2159 0.42 +2.5 2,587,623 - Shewana3_2159 0.42 +1.5 2,587,635 + Shewana3_2159 0.45 -1.0 2,587,651 + Shewana3_2159 0.48 +0.2 2,587,681 + Shewana3_2159 0.54 -0.7 2,587,895 + +0.3 2,588,416 - Shewana3_2160 0.20 +0.2 2,588,416 - Shewana3_2160 0.20 +3.1 2,588,798 + Shewana3_2160 0.37 +2.5
Or see this region's nucleotide sequence