Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1897 and Shewana3_1898 are separated by 704 nucleotides Shewana3_1898 and Shewana3_1899 are separated by 44 nucleotides Shewana3_1899 and Shewana3_1900 are separated by 2 nucleotides
Shewana3_1897: Shewana3_1897 - ATP-dependent helicase HrpA (RefSeq), at 2,250,421 to 2,254,302
_1897
Shewana3_1898: Shewana3_1898 - hypothetical protein (RefSeq), at 2,255,007 to 2,255,165
_1898
Shewana3_1899: Shewana3_1899 - NapD family protein (RefSeq), at 2,255,210 to 2,255,467
_1899
Shewana3_1900: Shewana3_1900 - nitrate reductase catalytic subunit (RefSeq), at 2,255,470 to 2,257,959
_1900
Position (kb)
2255
2256 Strain fitness (log2 ratio)
-1
0
1
2 at 2254.040 kb on + strand at 2254.258 kb on - strand at 2254.594 kb on + strand at 2254.663 kb on - strand at 2254.901 kb on + strand at 2254.901 kb on + strand at 2254.964 kb on + strand at 2255.067 kb on - strand, within Shewana3_1898 at 2255.081 kb on + strand, within Shewana3_1898 at 2255.081 kb on + strand, within Shewana3_1898 at 2255.315 kb on + strand, within Shewana3_1899 at 2255.575 kb on - strand at 2255.575 kb on - strand at 2255.599 kb on + strand at 2255.607 kb on - strand at 2255.773 kb on - strand, within Shewana3_1900 at 2256.011 kb on - strand, within Shewana3_1900
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 2,254,040 + +1.4 2,254,258 - +0.6 2,254,594 + +1.0 2,254,663 - +0.6 2,254,901 + +1.6 2,254,901 + +0.3 2,254,964 + +2.0 2,255,067 - Shewana3_1898 0.38 +1.6 2,255,081 + Shewana3_1898 0.47 +0.7 2,255,081 + Shewana3_1898 0.47 +1.9 2,255,315 + Shewana3_1899 0.41 +0.2 2,255,575 - +2.5 2,255,575 - +2.5 2,255,599 + +0.3 2,255,607 - +1.5 2,255,773 - Shewana3_1900 0.12 +1.5 2,256,011 - Shewana3_1900 0.22 +1.0
Or see this region's nucleotide sequence