Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1866 and Shewana3_1867 are separated by 129 nucleotides Shewana3_1867 and Shewana3_1868 overlap by 1 nucleotides
Shewana3_1866: Shewana3_1866 - lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (RefSeq), at 2,211,053 to 2,211,991
_1866
Shewana3_1867: Shewana3_1867 - hydrogenase nickel insertion protein HypA (RefSeq), at 2,212,121 to 2,212,477
_1867
Shewana3_1868: Shewana3_1868 - hydrogenase expression/formation protein HypE (RefSeq), at 2,212,477 to 2,213,496
_1868
Position (kb)
2212
2213 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2211.196 kb on - strand at 2211.523 kb on + strand, within Shewana3_1866 at 2211.569 kb on + strand, within Shewana3_1866 at 2212.204 kb on - strand, within Shewana3_1867 at 2212.209 kb on + strand, within Shewana3_1867 at 2212.494 kb on - strand at 2212.520 kb on - strand at 2212.649 kb on - strand, within Shewana3_1868 at 2212.717 kb on + strand, within Shewana3_1868 at 2212.721 kb on - strand, within Shewana3_1868 at 2213.172 kb on - strand, within Shewana3_1868 at 2213.334 kb on + strand, within Shewana3_1868 at 2213.382 kb on + strand, within Shewana3_1868 at 2213.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 2,211,196 - +1.6 2,211,523 + Shewana3_1866 0.50 +1.6 2,211,569 + Shewana3_1866 0.55 +1.0 2,212,204 - Shewana3_1867 0.23 -1.9 2,212,209 + Shewana3_1867 0.25 +0.3 2,212,494 - +0.8 2,212,520 - +0.1 2,212,649 - Shewana3_1868 0.17 +1.1 2,212,717 + Shewana3_1868 0.24 +1.6 2,212,721 - Shewana3_1868 0.24 +0.3 2,213,172 - Shewana3_1868 0.68 +2.6 2,213,334 + Shewana3_1868 0.84 +1.6 2,213,382 + Shewana3_1868 0.89 +1.6 2,213,448 + +1.6
Or see this region's nucleotide sequence