Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1522 and Shewana3_1523 are separated by 189 nucleotides Shewana3_1523 and Shewana3_1524 are separated by 3 nucleotides Shewana3_1524 and Shewana3_1525 overlap by 8 nucleotides Shewana3_1525 and Shewana3_1526 are separated by 113 nucleotides
Shewana3_1522: Shewana3_1522 - translation factor SUA5 (RefSeq), at 1,773,237 to 1,773,857
_1522
Shewana3_1523: Shewana3_1523 - condensin subunit ScpA (RefSeq), at 1,774,047 to 1,774,835
_1523
Shewana3_1524: Shewana3_1524 - condensin subunit ScpB (RefSeq), at 1,774,839 to 1,775,435
_1524
Shewana3_1525: Shewana3_1525 - ribosomal large subunit pseudouridine synthase B (RefSeq), at 1,775,428 to 1,776,303
_1525
Shewana3_1526: Shewana3_1526 - hypothetical protein (RefSeq), at 1,776,417 to 1,777,379
_1526
Position (kb)
1774
1775
1776 Strain fitness (log2 ratio)
-1
0
1
2 at 1773.967 kb on + strand at 1773.982 kb on + strand at 1773.982 kb on + strand at 1774.003 kb on + strand at 1774.326 kb on - strand, within Shewana3_1523 at 1774.342 kb on - strand, within Shewana3_1523 at 1774.517 kb on + strand, within Shewana3_1523 at 1774.722 kb on + strand, within Shewana3_1523 at 1774.730 kb on - strand, within Shewana3_1523 at 1774.972 kb on - strand, within Shewana3_1524 at 1775.002 kb on - strand, within Shewana3_1524 at 1775.081 kb on + strand, within Shewana3_1524 at 1775.122 kb on + strand, within Shewana3_1524 at 1775.449 kb on + strand at 1775.674 kb on + strand, within Shewana3_1525 at 1775.742 kb on - strand, within Shewana3_1525 at 1775.759 kb on + strand, within Shewana3_1525 at 1775.915 kb on + strand, within Shewana3_1525 at 1776.107 kb on + strand, within Shewana3_1525 at 1776.214 kb on + strand, within Shewana3_1525 at 1776.216 kb on + strand at 1776.224 kb on - strand at 1776.251 kb on + strand at 1776.270 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 1,773,967 + +0.8 1,773,982 + -0.4 1,773,982 + -0.2 1,774,003 + +0.2 1,774,326 - Shewana3_1523 0.35 +0.8 1,774,342 - Shewana3_1523 0.37 +0.4 1,774,517 + Shewana3_1523 0.60 +0.8 1,774,722 + Shewana3_1523 0.86 -0.9 1,774,730 - Shewana3_1523 0.87 +0.8 1,774,972 - Shewana3_1524 0.22 +1.4 1,775,002 - Shewana3_1524 0.27 +2.4 1,775,081 + Shewana3_1524 0.41 -0.4 1,775,122 + Shewana3_1524 0.47 -1.4 1,775,449 + +0.3 1,775,674 + Shewana3_1525 0.28 +0.3 1,775,742 - Shewana3_1525 0.36 -0.6 1,775,759 + Shewana3_1525 0.38 +0.5 1,775,915 + Shewana3_1525 0.56 -0.9 1,776,107 + Shewana3_1525 0.78 -0.3 1,776,214 + Shewana3_1525 0.90 +0.5 1,776,216 + +0.2 1,776,224 - +2.4 1,776,251 + +0.4 1,776,270 + +2.0
Or see this region's nucleotide sequence