Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secF and Shewana3_1439 are separated by 256 nucleotides Shewana3_1439 and Shewana3_1440 are separated by 269 nucleotides Shewana3_1440 and Shewana3_1441 are separated by 34 nucleotides
Shewana3_1438: secF - preprotein translocase subunit SecF (RefSeq), at 1,667,661 to 1,668,608
secF
Shewana3_1439: Shewana3_1439 - hypothetical protein (RefSeq), at 1,668,865 to 1,669,182
_1439
Shewana3_1440: Shewana3_1440 - rhodanese domain-containing protein (RefSeq), at 1,669,452 to 1,669,811
_1440
Shewana3_1441: Shewana3_1441 - siroheme synthase (RefSeq), at 1,669,846 to 1,670,757
_1441
Position (kb) 1668
1669
1670 Strain fitness (log2 ratio) -2
-1
0
1 at 1667.877 kb on + strand, within secF at 1667.959 kb on + strand, within secF at 1668.257 kb on + strand, within secF at 1668.828 kb on - strand at 1669.109 kb on + strand, within Shewana3_1439 at 1669.332 kb on - strand at 1669.463 kb on - strand at 1669.489 kb on - strand, within Shewana3_1440 at 1669.636 kb on + strand, within Shewana3_1440 at 1669.995 kb on + strand, within Shewana3_1441
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 1,667,877 + secF Shewana3_1438 0.23 +0.9 1,667,959 + secF Shewana3_1438 0.31 -0.5 1,668,257 + secF Shewana3_1438 0.63 +0.3 1,668,828 - -1.8 1,669,109 + Shewana3_1439 0.77 -0.5 1,669,332 - +0.2 1,669,463 - +0.1 1,669,489 - Shewana3_1440 0.10 +1.3 1,669,636 + Shewana3_1440 0.51 +0.3 1,669,995 + Shewana3_1441 0.16 +1.3
Or see this region's nucleotide sequence