Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1161 and Shewana3_1162 are separated by 149 nucleotides Shewana3_1162 and Shewana3_1163 are separated by 268 nucleotides Shewana3_1163 and Shewana3_1164 are separated by 201 nucleotides
Shewana3_1161: Shewana3_1161 - diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) (RefSeq), at 1,369,855 to 1,372,491
_1161
Shewana3_1162: Shewana3_1162 - DEAD/DEAH box helicase domain-containing protein (RefSeq), at 1,372,641 to 1,373,870
_1162
Shewana3_1163: Shewana3_1163 - hypothetical protein (RefSeq), at 1,374,139 to 1,374,648
_1163
Shewana3_1164: Shewana3_1164 - hypothetical protein (RefSeq), at 1,374,850 to 1,375,803
_1164
Position (kb)
1372
1373
1374 Strain fitness (log2 ratio)
-1
0
1 at 1371.666 kb on + strand, within Shewana3_1161 at 1372.100 kb on + strand, within Shewana3_1161 at 1372.154 kb on + strand, within Shewana3_1161 at 1372.435 kb on + strand at 1372.435 kb on + strand at 1372.437 kb on + strand at 1372.677 kb on + strand at 1372.973 kb on - strand, within Shewana3_1162 at 1373.079 kb on - strand, within Shewana3_1162 at 1373.180 kb on + strand, within Shewana3_1162 at 1373.390 kb on - strand, within Shewana3_1162 at 1373.575 kb on - strand, within Shewana3_1162 at 1373.698 kb on + strand, within Shewana3_1162 at 1374.007 kb on - strand at 1374.248 kb on + strand, within Shewana3_1163 at 1374.396 kb on + strand, within Shewana3_1163
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 1,371,666 + Shewana3_1161 0.69 +1.2 1,372,100 + Shewana3_1161 0.85 +0.1 1,372,154 + Shewana3_1161 0.87 -0.2 1,372,435 + -0.5 1,372,435 + +0.2 1,372,437 + +0.8 1,372,677 + +0.5 1,372,973 - Shewana3_1162 0.27 +0.9 1,373,079 - Shewana3_1162 0.36 -1.3 1,373,180 + Shewana3_1162 0.44 -0.5 1,373,390 - Shewana3_1162 0.61 -0.4 1,373,575 - Shewana3_1162 0.76 -0.5 1,373,698 + Shewana3_1162 0.86 -1.5 1,374,007 - +0.3 1,374,248 + Shewana3_1163 0.21 -0.0 1,374,396 + Shewana3_1163 0.50 -1.0
Or see this region's nucleotide sequence