Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1125 and recA are separated by 382 nucleotides recA and Shewana3_1127 are separated by 113 nucleotides Shewana3_1127 and alaS are separated by 275 nucleotides
Shewana3_1125: Shewana3_1125 - DNA mismatch repair protein MutS (RefSeq), at 1,323,429 to 1,326,014
_1125
Shewana3_1126: recA - recombinase A (RefSeq), at 1,326,397 to 1,327,470
recA
Shewana3_1127: Shewana3_1127 - regulatory protein RecX (RefSeq), at 1,327,584 to 1,327,955
_1127
Shewana3_1128: alaS - alanyl-tRNA synthetase (RefSeq), at 1,328,231 to 1,330,855
alaS
Position (kb)
1326
1327
1328 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1325.700 kb on - strand, within Shewana3_1125 at 1325.775 kb on + strand at 1325.775 kb on + strand at 1325.783 kb on - strand at 1325.872 kb on + strand at 1325.892 kb on - strand at 1325.894 kb on + strand at 1325.902 kb on - strand at 1325.902 kb on - strand at 1326.052 kb on + strand at 1326.154 kb on + strand at 1326.261 kb on + strand at 1326.263 kb on + strand at 1326.269 kb on - strand at 1326.294 kb on - strand at 1326.377 kb on + strand at 1326.377 kb on + strand at 1326.385 kb on - strand at 1326.385 kb on - strand at 1326.476 kb on + strand at 1326.588 kb on + strand, within recA at 1326.626 kb on + strand, within recA at 1326.634 kb on - strand, within recA at 1326.942 kb on + strand, within recA at 1327.047 kb on + strand, within recA at 1327.047 kb on + strand, within recA at 1327.146 kb on - strand, within recA at 1327.294 kb on + strand, within recA at 1327.294 kb on + strand, within recA at 1327.302 kb on - strand, within recA at 1327.302 kb on - strand, within recA at 1327.366 kb on - strand at 1327.401 kb on + strand at 1327.464 kb on + strand at 1327.964 kb on - strand at 1327.976 kb on + strand at 1327.981 kb on + strand at 1328.124 kb on + strand at 1328.124 kb on + strand at 1328.124 kb on + strand at 1328.132 kb on - strand at 1328.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 1,325,700 - Shewana3_1125 0.88 +0.7 1,325,775 + +1.3 1,325,775 + +0.3 1,325,783 - +0.1 1,325,872 + -0.5 1,325,892 - -0.5 1,325,894 + -1.0 1,325,902 - +0.7 1,325,902 - +1.4 1,326,052 + -0.3 1,326,154 + -0.6 1,326,261 + -0.7 1,326,263 + -2.6 1,326,269 - -2.2 1,326,294 - +0.2 1,326,377 + -2.4 1,326,377 + -5.3 1,326,385 - -0.5 1,326,385 - -0.0 1,326,476 + -2.6 1,326,588 + recA Shewana3_1126 0.18 -1.7 1,326,626 + recA Shewana3_1126 0.21 -4.0 1,326,634 - recA Shewana3_1126 0.22 -0.0 1,326,942 + recA Shewana3_1126 0.51 -2.4 1,327,047 + recA Shewana3_1126 0.61 -4.8 1,327,047 + recA Shewana3_1126 0.61 -5.8 1,327,146 - recA Shewana3_1126 0.70 +0.7 1,327,294 + recA Shewana3_1126 0.84 -3.7 1,327,294 + recA Shewana3_1126 0.84 -5.3 1,327,302 - recA Shewana3_1126 0.84 +1.3 1,327,302 - recA Shewana3_1126 0.84 +2.3 1,327,366 - -0.7 1,327,401 + -0.0 1,327,464 + -0.0 1,327,964 - +1.3 1,327,976 + +0.1 1,327,981 + -0.5 1,328,124 + +0.2 1,328,124 + -0.3 1,328,124 + +0.1 1,328,132 - +1.6 1,328,132 - +0.9
Or see this region's nucleotide sequence