Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1018 and secD are separated by 377 nucleotides secD and secF are separated by 2 nucleotides secF and Shewana3_1021 are separated by 57 nucleotides
Shewana3_1018: Shewana3_1018 - hypothetical protein (RefSeq), at 1,189,315 to 1,189,842
_1018
Shewana3_1019: secD - preprotein translocase subunit SecD (RefSeq), at 1,190,220 to 1,192,094
secD
Shewana3_1020: secF - preprotein translocase subunit SecF (RefSeq), at 1,192,097 to 1,192,981
secF
Shewana3_1021: Shewana3_1021 - hypothetical protein (RefSeq), at 1,193,039 to 1,193,338
_1021
Position (kb)
1190
1191
1192
1193 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1189.501 kb on - strand, within Shewana3_1018 at 1189.622 kb on - strand, within Shewana3_1018 at 1190.095 kb on - strand at 1190.129 kb on + strand at 1190.309 kb on - strand at 1190.311 kb on - strand at 1190.346 kb on + strand at 1190.375 kb on - strand at 1190.424 kb on - strand, within secD at 1190.482 kb on + strand, within secD at 1190.496 kb on - strand, within secD at 1190.522 kb on - strand, within secD at 1190.582 kb on + strand, within secD at 1190.582 kb on + strand, within secD at 1190.582 kb on + strand, within secD at 1190.590 kb on - strand, within secD at 1190.590 kb on - strand, within secD at 1190.617 kb on + strand, within secD at 1191.251 kb on - strand, within secD at 1191.403 kb on - strand, within secD at 1191.579 kb on - strand, within secD at 1191.747 kb on + strand, within secD at 1191.755 kb on - strand, within secD at 1191.879 kb on + strand, within secD at 1191.879 kb on + strand, within secD at 1192.049 kb on - strand at 1192.056 kb on + strand at 1192.056 kb on + strand at 1192.087 kb on + strand at 1192.095 kb on - strand at 1192.095 kb on - strand at 1192.389 kb on + strand, within secF at 1192.397 kb on - strand, within secF at 1192.447 kb on + strand, within secF at 1192.481 kb on - strand, within secF at 1192.491 kb on - strand, within secF at 1192.498 kb on + strand, within secF at 1192.797 kb on + strand, within secF at 1192.830 kb on + strand, within secF at 1192.945 kb on + strand at 1192.945 kb on + strand at 1192.953 kb on - strand at 1192.955 kb on + strand at 1193.046 kb on + strand at 1193.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 1,189,501 - Shewana3_1018 0.35 +0.1 1,189,622 - Shewana3_1018 0.58 -0.6 1,190,095 - +0.4 1,190,129 + -0.5 1,190,309 - -0.1 1,190,311 - -1.0 1,190,346 + +0.4 1,190,375 - -0.9 1,190,424 - secD Shewana3_1019 0.11 +0.3 1,190,482 + secD Shewana3_1019 0.14 -0.3 1,190,496 - secD Shewana3_1019 0.15 -1.2 1,190,522 - secD Shewana3_1019 0.16 +2.3 1,190,582 + secD Shewana3_1019 0.19 +0.3 1,190,582 + secD Shewana3_1019 0.19 -0.7 1,190,582 + secD Shewana3_1019 0.19 -1.4 1,190,590 - secD Shewana3_1019 0.20 -1.3 1,190,590 - secD Shewana3_1019 0.20 -0.1 1,190,617 + secD Shewana3_1019 0.21 +0.7 1,191,251 - secD Shewana3_1019 0.55 -0.6 1,191,403 - secD Shewana3_1019 0.63 -2.5 1,191,579 - secD Shewana3_1019 0.72 +0.2 1,191,747 + secD Shewana3_1019 0.81 -0.9 1,191,755 - secD Shewana3_1019 0.82 -0.0 1,191,879 + secD Shewana3_1019 0.88 -0.2 1,191,879 + secD Shewana3_1019 0.88 +0.3 1,192,049 - -1.3 1,192,056 + +0.1 1,192,056 + -0.5 1,192,087 + -0.3 1,192,095 - +1.5 1,192,095 - -0.4 1,192,389 + secF Shewana3_1020 0.33 +0.1 1,192,397 - secF Shewana3_1020 0.34 +0.3 1,192,447 + secF Shewana3_1020 0.40 -0.1 1,192,481 - secF Shewana3_1020 0.43 +0.1 1,192,491 - secF Shewana3_1020 0.45 -0.1 1,192,498 + secF Shewana3_1020 0.45 -0.7 1,192,797 + secF Shewana3_1020 0.79 -0.3 1,192,830 + secF Shewana3_1020 0.83 -0.5 1,192,945 + -0.7 1,192,945 + +0.2 1,192,953 - +2.3 1,192,955 + -0.2 1,193,046 + -0.6 1,193,054 - +0.5
Or see this region's nucleotide sequence