Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0871 and Shewana3_0872 are separated by 41 nucleotides Shewana3_0872 and Shewana3_0873 are separated by 264 nucleotides Shewana3_0873 and Shewana3_0874 are separated by 123 nucleotides
Shewana3_0871: Shewana3_0871 - protoporphyrinogen oxidase (RefSeq), at 1,017,066 to 1,017,587
_0871
Shewana3_0872: Shewana3_0872 - hypothetical protein (RefSeq), at 1,017,629 to 1,018,156
_0872
Shewana3_0873: Shewana3_0873 - putative lipoprotein (RefSeq), at 1,018,421 to 1,019,428
_0873
Shewana3_0874: Shewana3_0874 - DSBA oxidoreductase (RefSeq), at 1,019,552 to 1,020,304
_0874
Position (kb)
1018
1019
1020 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1017.714 kb on + strand, within Shewana3_0872 at 1017.746 kb on - strand, within Shewana3_0872 at 1017.746 kb on - strand, within Shewana3_0872 at 1017.799 kb on - strand, within Shewana3_0872 at 1017.916 kb on + strand, within Shewana3_0872 at 1017.916 kb on + strand, within Shewana3_0872 at 1017.955 kb on + strand, within Shewana3_0872 at 1017.968 kb on - strand, within Shewana3_0872 at 1018.001 kb on + strand, within Shewana3_0872 at 1018.290 kb on + strand at 1018.298 kb on - strand at 1018.335 kb on + strand at 1018.490 kb on - strand at 1018.702 kb on + strand, within Shewana3_0873 at 1019.004 kb on + strand, within Shewana3_0873 at 1019.012 kb on - strand, within Shewana3_0873 at 1019.396 kb on + strand at 1019.429 kb on + strand at 1019.872 kb on + strand, within Shewana3_0874 at 1020.039 kb on + strand, within Shewana3_0874 at 1020.093 kb on + strand, within Shewana3_0874 at 1020.100 kb on + strand, within Shewana3_0874 at 1020.186 kb on - strand, within Shewana3_0874 at 1020.295 kb on - strand at 1020.295 kb on - strand at 1020.310 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 1,017,714 + Shewana3_0872 0.16 +3.0 1,017,746 - Shewana3_0872 0.22 -0.0 1,017,746 - Shewana3_0872 0.22 -1.0 1,017,799 - Shewana3_0872 0.32 -0.4 1,017,916 + Shewana3_0872 0.54 -0.2 1,017,916 + Shewana3_0872 0.54 +2.5 1,017,955 + Shewana3_0872 0.62 +0.4 1,017,968 - Shewana3_0872 0.64 -0.6 1,018,001 + Shewana3_0872 0.70 +1.2 1,018,290 + -0.4 1,018,298 - -0.0 1,018,335 + +0.2 1,018,490 - -1.3 1,018,702 + Shewana3_0873 0.28 -1.0 1,019,004 + Shewana3_0873 0.58 +0.4 1,019,012 - Shewana3_0873 0.59 -0.8 1,019,396 + -0.6 1,019,429 + -0.1 1,019,872 + Shewana3_0874 0.42 -0.2 1,020,039 + Shewana3_0874 0.65 +0.1 1,020,093 + Shewana3_0874 0.72 -1.7 1,020,100 + Shewana3_0874 0.73 -0.4 1,020,186 - Shewana3_0874 0.84 +1.1 1,020,295 - -0.0 1,020,295 - +0.2 1,020,310 + -0.8
Or see this region's nucleotide sequence