Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0849 and Shewana3_0850 overlap by 4 nucleotides Shewana3_0850 and Shewana3_0851 are separated by 153 nucleotides Shewana3_0851 and Shewana3_0852 are separated by 86 nucleotides Shewana3_0852 and pntB are separated by 154 nucleotides
Shewana3_0849: Shewana3_0849 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (RefSeq), at 986,407 to 987,837
_0849
Shewana3_0850: Shewana3_0850 - hypothetical protein (RefSeq), at 987,834 to 988,661
_0850
Shewana3_0851: Shewana3_0851 - TetR family transcriptional regulator (RefSeq), at 988,815 to 989,294
_0851
Shewana3_0852: Shewana3_0852 - hypothetical protein (RefSeq), at 989,381 to 989,770
_0852
Shewana3_0853: pntB - pyridine nucleotide transhydrogenase (RefSeq), at 989,925 to 991,409
pntB
Position (kb) 988
989
990 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 987.859 kb on + strand at 988.012 kb on + strand, within Shewana3_0850 at 988.019 kb on + strand, within Shewana3_0850 at 988.027 kb on - strand, within Shewana3_0850 at 988.768 kb on + strand at 988.786 kb on - strand at 988.832 kb on + strand at 988.905 kb on + strand, within Shewana3_0851 at 989.106 kb on + strand, within Shewana3_0851 at 989.159 kb on + strand, within Shewana3_0851 at 989.167 kb on - strand, within Shewana3_0851 at 989.557 kb on + strand, within Shewana3_0852 at 989.576 kb on + strand, within Shewana3_0852 at 989.720 kb on - strand, within Shewana3_0852 at 989.767 kb on + strand at 989.856 kb on + strand at 989.864 kb on - strand at 989.864 kb on - strand at 989.923 kb on - strand at 990.014 kb on - strand at 990.105 kb on + strand, within pntB at 990.240 kb on + strand, within pntB
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 987,859 + +0.0 988,012 + Shewana3_0850 0.21 +0.6 988,019 + Shewana3_0850 0.22 -0.3 988,027 - Shewana3_0850 0.23 -0.6 988,768 + -1.0 988,786 - -1.5 988,832 + -2.6 988,905 + Shewana3_0851 0.19 -1.9 989,106 + Shewana3_0851 0.61 -3.0 989,159 + Shewana3_0851 0.72 -0.8 989,167 - Shewana3_0851 0.73 +2.3 989,557 + Shewana3_0852 0.45 +0.2 989,576 + Shewana3_0852 0.50 -0.2 989,720 - Shewana3_0852 0.87 -0.4 989,767 + -0.5 989,856 + -1.4 989,864 - -0.4 989,864 - -0.7 989,923 - -0.2 990,014 - -0.3 990,105 + pntB Shewana3_0853 0.12 +0.7 990,240 + pntB Shewana3_0853 0.21 +0.8
Or see this region's nucleotide sequence