Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0531 and Shewana3_0532 are separated by 500 nucleotides Shewana3_0532 and Shewana3_0533 are separated by 40 nucleotides Shewana3_0533 and Shewana3_0534 are separated by 65 nucleotides
Shewana3_0531: Shewana3_0531 - lysine exporter protein LysE/YggA (RefSeq), at 625,299 to 625,910
_0531
Shewana3_0532: Shewana3_0532 - ArsR family transcriptional regulator (RefSeq), at 626,411 to 626,755
_0532
Shewana3_0533: Shewana3_0533 - bile acid:sodium symporter (RefSeq), at 626,796 to 627,815
_0533
Shewana3_0534: Shewana3_0534 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 627,881 to 628,894
_0534
Position (kb)
626
627
628 Strain fitness (log2 ratio)
-1
0
1
2 at 625.796 kb on - strand, within Shewana3_0531 at 625.808 kb on + strand, within Shewana3_0531 at 625.854 kb on - strand at 625.856 kb on + strand at 625.856 kb on + strand at 625.856 kb on + strand at 625.856 kb on + strand at 626.033 kb on + strand at 626.052 kb on + strand at 626.053 kb on - strand at 626.107 kb on - strand at 626.217 kb on - strand at 626.455 kb on + strand, within Shewana3_0532 at 626.463 kb on - strand, within Shewana3_0532 at 626.535 kb on - strand, within Shewana3_0532 at 626.672 kb on - strand, within Shewana3_0532 at 626.736 kb on - strand at 626.827 kb on + strand at 626.848 kb on + strand at 626.964 kb on - strand, within Shewana3_0533 at 627.226 kb on + strand, within Shewana3_0533 at 627.779 kb on - strand at 627.932 kb on - strand at 628.165 kb on - strand, within Shewana3_0534 at 628.172 kb on - strand, within Shewana3_0534 at 628.299 kb on - strand, within Shewana3_0534 at 628.407 kb on + strand, within Shewana3_0534 at 628.546 kb on + strand, within Shewana3_0534 at 628.552 kb on + strand, within Shewana3_0534 at 628.654 kb on - strand, within Shewana3_0534 at 628.724 kb on - strand, within Shewana3_0534 at 628.798 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 625,796 - Shewana3_0531 0.81 +1.8 625,808 + Shewana3_0531 0.83 -0.4 625,854 - -0.8 625,856 + -0.2 625,856 + -1.4 625,856 + -0.2 625,856 + +1.7 626,033 + +0.6 626,052 + -0.6 626,053 - -0.5 626,107 - -1.4 626,217 - -0.5 626,455 + Shewana3_0532 0.13 -0.1 626,463 - Shewana3_0532 0.15 +0.4 626,535 - Shewana3_0532 0.36 -0.2 626,672 - Shewana3_0532 0.76 +0.1 626,736 - -0.4 626,827 + -0.3 626,848 + +0.2 626,964 - Shewana3_0533 0.16 +0.0 627,226 + Shewana3_0533 0.42 -1.6 627,779 - +0.0 627,932 - +0.4 628,165 - Shewana3_0534 0.28 -0.6 628,172 - Shewana3_0534 0.29 +0.0 628,299 - Shewana3_0534 0.41 -0.8 628,407 + Shewana3_0534 0.52 -0.1 628,546 + Shewana3_0534 0.66 +0.2 628,552 + Shewana3_0534 0.66 -0.5 628,654 - Shewana3_0534 0.76 -0.5 628,724 - Shewana3_0534 0.83 -0.5 628,798 + -0.1
Or see this region's nucleotide sequence