Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aceE and Shewana3_0427 are separated by 12 nucleotides Shewana3_0427 and Shewana3_0428 are separated by 149 nucleotides
Shewana3_0426: aceE - pyruvate dehydrogenase subunit E1 (RefSeq), at 485,256 to 487,922
aceE
Shewana3_0427: Shewana3_0427 - pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase (RefSeq), at 487,935 to 489,941
_0427
Shewana3_0428: Shewana3_0428 - dihydrolipoamide dehydrogenase (RefSeq), at 490,091 to 491,518
_0428
Position (kb) 487
488
489
490 Strain fitness (log2 ratio) -1
0
1
2
3 at 487.017 kb on + strand, within aceE at 487.088 kb on + strand, within aceE at 487.096 kb on - strand, within aceE at 487.192 kb on - strand, within aceE at 487.210 kb on - strand, within aceE at 487.230 kb on - strand, within aceE at 487.493 kb on - strand, within aceE at 487.679 kb on + strand at 487.717 kb on + strand at 487.725 kb on + strand at 487.733 kb on - strand at 487.780 kb on + strand at 487.800 kb on - strand at 487.851 kb on + strand at 487.991 kb on - strand at 488.097 kb on + strand at 488.193 kb on + strand, within Shewana3_0427 at 488.340 kb on + strand, within Shewana3_0427 at 488.506 kb on + strand, within Shewana3_0427 at 488.514 kb on - strand, within Shewana3_0427 at 488.725 kb on + strand, within Shewana3_0427 at 488.733 kb on - strand, within Shewana3_0427 at 488.835 kb on + strand, within Shewana3_0427 at 488.914 kb on - strand, within Shewana3_0427 at 489.196 kb on - strand, within Shewana3_0427 at 489.299 kb on - strand, within Shewana3_0427 at 489.307 kb on + strand, within Shewana3_0427 at 489.363 kb on - strand, within Shewana3_0427 at 489.472 kb on - strand at 489.486 kb on + strand, within Shewana3_0427 at 489.546 kb on - strand, within Shewana3_0427 at 489.562 kb on + strand, within Shewana3_0427 at 489.562 kb on + strand, within Shewana3_0427 at 489.562 kb on + strand, within Shewana3_0427 at 489.570 kb on - strand, within Shewana3_0427 at 489.659 kb on + strand, within Shewana3_0427 at 489.675 kb on + strand, within Shewana3_0427
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 487,017 + aceE Shewana3_0426 0.66 +0.5 487,088 + aceE Shewana3_0426 0.69 +0.9 487,096 - aceE Shewana3_0426 0.69 -0.5 487,192 - aceE Shewana3_0426 0.73 +0.8 487,210 - aceE Shewana3_0426 0.73 +0.0 487,230 - aceE Shewana3_0426 0.74 -0.3 487,493 - aceE Shewana3_0426 0.84 +2.3 487,679 + +0.5 487,717 + +1.0 487,725 + +0.5 487,733 - +0.8 487,780 + +0.5 487,800 - +1.3 487,851 + +2.8 487,991 - +2.3 488,097 + +0.7 488,193 + Shewana3_0427 0.13 +0.4 488,340 + Shewana3_0427 0.20 +0.2 488,506 + Shewana3_0427 0.28 +0.7 488,514 - Shewana3_0427 0.29 +0.3 488,725 + Shewana3_0427 0.39 +0.8 488,733 - Shewana3_0427 0.40 -0.1 488,835 + Shewana3_0427 0.45 +1.1 488,914 - Shewana3_0427 0.49 +0.2 489,196 - Shewana3_0427 0.63 +1.3 489,299 - Shewana3_0427 0.68 +0.8 489,307 + Shewana3_0427 0.68 +0.6 489,363 - Shewana3_0427 0.71 +0.9 489,472 - +2.3 489,486 + Shewana3_0427 0.77 +1.0 489,546 - Shewana3_0427 0.80 +0.5 489,562 + Shewana3_0427 0.81 +0.9 489,562 + Shewana3_0427 0.81 +1.0 489,562 + Shewana3_0427 0.81 +1.1 489,570 - Shewana3_0427 0.81 +0.9 489,659 + Shewana3_0427 0.86 +0.3 489,675 + Shewana3_0427 0.87 +0.4
Or see this region's nucleotide sequence