Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0412 and Shewana3_0413 are separated by 147 nucleotides Shewana3_0413 and Shewana3_0414 are separated by 34 nucleotides Shewana3_0414 and Shewana3_0415 are separated by 107 nucleotides Shewana3_0415 and coaE overlap by 8 nucleotides
Shewana3_0412: Shewana3_0412 - hypothetical protein (RefSeq), at 471,568 to 472,104
_0412
Shewana3_0413: Shewana3_0413 - mutator MutT protein (RefSeq), at 472,252 to 472,650
_0413
Shewana3_0414: Shewana3_0414 - hypothetical protein (RefSeq), at 472,685 to 472,894
_0414
Shewana3_0415: Shewana3_0415 - hypothetical protein (RefSeq), at 473,002 to 473,736
_0415
Shewana3_0416: coaE - dephospho-CoA kinase (RefSeq), at 473,729 to 474,346
coaE
Position (kb) 472
473 Strain fitness (log2 ratio) -2
-1
0
1 at 471.725 kb on + strand, within Shewana3_0412 at 471.969 kb on + strand, within Shewana3_0412 at 472.172 kb on - strand at 472.445 kb on - strand, within Shewana3_0413 at 472.503 kb on - strand, within Shewana3_0413 at 472.622 kb on - strand at 472.710 kb on + strand, within Shewana3_0414 at 472.744 kb on + strand, within Shewana3_0414 at 472.984 kb on - strand at 472.991 kb on - strand at 473.042 kb on + strand at 473.072 kb on - strand at 473.219 kb on - strand, within Shewana3_0415 at 473.651 kb on + strand, within Shewana3_0415 at 473.705 kb on + strand at 473.715 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 471,725 + Shewana3_0412 0.29 +1.3 471,969 + Shewana3_0412 0.75 -0.8 472,172 - -0.1 472,445 - Shewana3_0413 0.48 -0.1 472,503 - Shewana3_0413 0.63 -0.5 472,622 - +0.5 472,710 + Shewana3_0414 0.12 -0.5 472,744 + Shewana3_0414 0.28 -2.5 472,984 - -0.1 472,991 - -1.5 473,042 + -0.0 473,072 - -0.6 473,219 - Shewana3_0415 0.30 -1.3 473,651 + Shewana3_0415 0.88 -0.3 473,705 + -1.9 473,715 + +0.6
Or see this region's nucleotide sequence