Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0118 and Shewana3_0119 are separated by 25 nucleotides Shewana3_0119 and Shewana3_0120 are separated by 129 nucleotides Shewana3_0120 and Shewana3_0121 are separated by 4 nucleotides Shewana3_0121 and Shewana3_0122 are separated by 63 nucleotides
Shewana3_0118: Shewana3_0118 - lysine exporter protein LysE/YggA (RefSeq), at 134,067 to 134,690
_0118
Shewana3_0119: Shewana3_0119 - phospholipid/glycerol acyltransferase (RefSeq), at 134,716 to 135,276
_0119
Shewana3_0120: Shewana3_0120 - hypothetical protein (RefSeq), at 135,406 to 135,855
_0120
Shewana3_0121: Shewana3_0121 - hypothetical protein (RefSeq), at 135,860 to 136,660
_0121
Shewana3_0122: Shewana3_0122 - hypothetical protein (RefSeq), at 136,724 to 138,367
_0122
Position (kb) 135
136 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 134.564 kb on - strand, within Shewana3_0118 at 134.564 kb on - strand, within Shewana3_0118 at 134.564 kb on - strand, within Shewana3_0118 at 134.569 kb on - strand, within Shewana3_0118 at 134.766 kb on + strand at 134.774 kb on - strand, within Shewana3_0119 at 134.774 kb on - strand, within Shewana3_0119 at 135.591 kb on + strand, within Shewana3_0120 at 135.591 kb on + strand, within Shewana3_0120 at 135.599 kb on - strand, within Shewana3_0120 at 135.693 kb on - strand, within Shewana3_0120 at 135.936 kb on - strand at 136.301 kb on - strand, within Shewana3_0121 at 136.457 kb on - strand, within Shewana3_0121 at 136.510 kb on - strand, within Shewana3_0121 at 136.663 kb on - strand at 136.721 kb on + strand at 136.729 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 134,564 - Shewana3_0118 0.80 +0.1 134,564 - Shewana3_0118 0.80 -0.1 134,564 - Shewana3_0118 0.80 -3.5 134,569 - Shewana3_0118 0.80 -0.5 134,766 + -0.5 134,774 - Shewana3_0119 0.10 +0.8 134,774 - Shewana3_0119 0.10 +0.1 135,591 + Shewana3_0120 0.41 -0.2 135,591 + Shewana3_0120 0.41 -0.4 135,599 - Shewana3_0120 0.43 -1.2 135,693 - Shewana3_0120 0.64 -0.5 135,936 - +0.0 136,301 - Shewana3_0121 0.55 -0.0 136,457 - Shewana3_0121 0.75 -0.4 136,510 - Shewana3_0121 0.81 -0.6 136,663 - -0.3 136,721 + -0.4 136,729 - -0.2
Or see this region's nucleotide sequence