Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3341 and Shew_3342 are separated by 200 nucleotides Shew_3342 and Shew_3343 overlap by 4 nucleotides Shew_3343 and Shew_3344 are separated by 9 nucleotides Shew_3344 and Shew_3345 overlap by 1 nucleotides
Shew_3341: Shew_3341 - diguanylate cyclase with GAF sensor (RefSeq), at 3,985,748 to 3,986,728
_3341
Shew_3342: Shew_3342 - two component Fis family transcriptional regulator (RefSeq), at 3,986,929 to 3,987,465
_3342
Shew_3343: Shew_3343 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 3,987,462 to 3,988,709
_3343
Shew_3344: Shew_3344 - dTDP-4-dehydrorhamnose reductase (RefSeq), at 3,988,719 to 3,989,642
_3344
Shew_3345: Shew_3345 - lipid kinase (RefSeq), at 3,989,642 to 3,990,565
_3345
Position (kb)
3987
3988
3989 Strain fitness (log2 ratio)
-1
0
1 at 3986.541 kb on + strand, within Shew_3341 at 3986.588 kb on + strand, within Shew_3341 at 3986.711 kb on - strand at 3986.836 kb on - strand at 3986.836 kb on - strand at 3986.949 kb on - strand at 3987.043 kb on + strand, within Shew_3342 at 3987.352 kb on - strand, within Shew_3342 at 3987.523 kb on - strand at 3988.158 kb on - strand, within Shew_3343 at 3988.209 kb on - strand, within Shew_3343 at 3988.293 kb on + strand, within Shew_3343 at 3988.488 kb on - strand, within Shew_3343 at 3988.514 kb on - strand, within Shew_3343 at 3988.603 kb on - strand at 3988.894 kb on + strand, within Shew_3344 at 3988.933 kb on + strand, within Shew_3344 at 3988.999 kb on + strand, within Shew_3344 at 3989.273 kb on - strand, within Shew_3344 at 3989.456 kb on - strand, within Shew_3344 at 3989.456 kb on - strand, within Shew_3344 at 3989.536 kb on - strand, within Shew_3344 at 3989.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 3,986,541 + Shew_3341 0.81 +0.4 3,986,588 + Shew_3341 0.86 +0.0 3,986,711 - -0.1 3,986,836 - -0.5 3,986,836 - +0.4 3,986,949 - -0.1 3,987,043 + Shew_3342 0.21 -0.1 3,987,352 - Shew_3342 0.79 -0.8 3,987,523 - -0.2 3,988,158 - Shew_3343 0.56 +0.7 3,988,209 - Shew_3343 0.60 -0.5 3,988,293 + Shew_3343 0.67 +0.0 3,988,488 - Shew_3343 0.82 -0.4 3,988,514 - Shew_3343 0.84 -1.3 3,988,603 - +0.4 3,988,894 + Shew_3344 0.19 -0.3 3,988,933 + Shew_3344 0.23 +0.4 3,988,999 + Shew_3344 0.30 +0.1 3,989,273 - Shew_3344 0.60 +0.1 3,989,456 - Shew_3344 0.80 +0.0 3,989,456 - Shew_3344 0.80 -0.1 3,989,536 - Shew_3344 0.88 -0.1 3,989,561 - -0.8
Or see this region's nucleotide sequence