Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3255 and Shew_3256 are separated by 32 nucleotides Shew_3256 and Shew_3257 overlap by 4 nucleotides Shew_3257 and Shew_3258 are separated by 251 nucleotides Shew_3258 and Shew_3259 are separated by 1 nucleotides
Shew_3255: Shew_3255 - hypothetical protein (RefSeq), at 3,892,287 to 3,892,760
_3255
Shew_3256: Shew_3256 - copper resistance B precursor (RefSeq), at 3,892,793 to 3,893,749
_3256
Shew_3257: Shew_3257 - CopA family copper resistance protein (RefSeq), at 3,893,746 to 3,895,491
_3257
Shew_3258: Shew_3258 - two component heavy metal response transcriptional regulator (RefSeq), at 3,895,743 to 3,896,459
_3258
Shew_3259: Shew_3259 - heavy metal sensor signal transduction histidine kinase (RefSeq), at 3,896,461 to 3,897,849
_3259
Position (kb)
3893
3894
3895
3896 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3892.909 kb on - strand, within Shew_3256 at 3892.961 kb on + strand, within Shew_3256 at 3893.027 kb on - strand, within Shew_3256 at 3893.069 kb on - strand, within Shew_3256 at 3893.182 kb on - strand, within Shew_3256 at 3893.801 kb on - strand at 3893.834 kb on - strand at 3894.020 kb on - strand, within Shew_3257 at 3894.058 kb on - strand, within Shew_3257 at 3894.156 kb on - strand, within Shew_3257 at 3894.198 kb on + strand, within Shew_3257 at 3894.264 kb on + strand, within Shew_3257 at 3894.304 kb on - strand, within Shew_3257 at 3894.367 kb on + strand, within Shew_3257 at 3894.437 kb on - strand, within Shew_3257 at 3894.613 kb on - strand, within Shew_3257 at 3894.779 kb on + strand, within Shew_3257 at 3894.827 kb on + strand, within Shew_3257 at 3895.257 kb on + strand, within Shew_3257 at 3895.265 kb on - strand, within Shew_3257 at 3895.265 kb on - strand, within Shew_3257 at 3895.265 kb on - strand, within Shew_3257 at 3895.265 kb on - strand, within Shew_3257 at 3895.324 kb on - strand at 3895.339 kb on + strand at 3895.382 kb on + strand at 3895.390 kb on - strand at 3895.664 kb on + strand at 3895.668 kb on + strand at 3895.762 kb on + strand at 3895.770 kb on - strand at 3895.777 kb on - strand at 3896.019 kb on + strand, within Shew_3258 at 3896.029 kb on + strand, within Shew_3258 at 3896.075 kb on - strand, within Shew_3258 at 3896.155 kb on - strand, within Shew_3258 at 3896.161 kb on + strand, within Shew_3258 at 3896.161 kb on + strand, within Shew_3258 at 3896.161 kb on + strand, within Shew_3258 at 3896.169 kb on - strand, within Shew_3258 at 3896.169 kb on - strand, within Shew_3258 at 3896.217 kb on - strand, within Shew_3258 at 3896.279 kb on + strand, within Shew_3258
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 3,892,909 - Shew_3256 0.12 +0.0 3,892,961 + Shew_3256 0.18 -0.6 3,893,027 - Shew_3256 0.24 -3.6 3,893,069 - Shew_3256 0.29 +0.2 3,893,182 - Shew_3256 0.41 +0.1 3,893,801 - -0.3 3,893,834 - -0.1 3,894,020 - Shew_3257 0.16 -0.0 3,894,058 - Shew_3257 0.18 -0.2 3,894,156 - Shew_3257 0.23 -0.1 3,894,198 + Shew_3257 0.26 -1.1 3,894,264 + Shew_3257 0.30 -0.8 3,894,304 - Shew_3257 0.32 -0.9 3,894,367 + Shew_3257 0.36 -0.0 3,894,437 - Shew_3257 0.40 +0.1 3,894,613 - Shew_3257 0.50 -0.2 3,894,779 + Shew_3257 0.59 -0.2 3,894,827 + Shew_3257 0.62 -0.0 3,895,257 + Shew_3257 0.87 +0.2 3,895,265 - Shew_3257 0.87 -0.0 3,895,265 - Shew_3257 0.87 +0.3 3,895,265 - Shew_3257 0.87 +1.1 3,895,265 - Shew_3257 0.87 -0.2 3,895,324 - -0.1 3,895,339 + -0.9 3,895,382 + -0.6 3,895,390 - +0.6 3,895,664 + +0.0 3,895,668 + -0.4 3,895,762 + +0.0 3,895,770 - +0.2 3,895,777 - +0.1 3,896,019 + Shew_3258 0.38 +0.1 3,896,029 + Shew_3258 0.40 +0.5 3,896,075 - Shew_3258 0.46 +0.2 3,896,155 - Shew_3258 0.57 +0.1 3,896,161 + Shew_3258 0.58 -0.0 3,896,161 + Shew_3258 0.58 +0.1 3,896,161 + Shew_3258 0.58 -0.3 3,896,169 - Shew_3258 0.59 +0.2 3,896,169 - Shew_3258 0.59 -0.2 3,896,217 - Shew_3258 0.66 -0.0 3,896,279 + Shew_3258 0.75 +0.2
Or see this region's nucleotide sequence