Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3178 and Shew_3179 are separated by 155 nucleotides Shew_3179 and Shew_3180 are separated by 8 nucleotides Shew_3180 and Shew_3181 are separated by 552 nucleotides
Shew_3178: Shew_3178 - Na+/H+ antiporter NhaC (RefSeq), at 3,783,257 to 3,784,588
_3178
Shew_3179: Shew_3179 - hypothetical protein (RefSeq), at 3,784,744 to 3,785,520
_3179
Shew_3180: Shew_3180 - pseudouridine synthase (RefSeq), at 3,785,529 to 3,786,245
_3180
Shew_3181: Shew_3181 - fumarate reductase/succinate dehydrogenase flavoprotein domain-containing protein (RefSeq), at 3,786,798 to 3,788,465
_3181
Position (kb)
3785
3786
3787 Strain fitness (log2 ratio)
-1
0
1
2 at 3784.573 kb on + strand at 3784.610 kb on - strand at 3784.650 kb on - strand at 3784.652 kb on + strand at 3784.660 kb on + strand at 3784.835 kb on - strand, within Shew_3179 at 3784.900 kb on - strand, within Shew_3179 at 3785.057 kb on + strand, within Shew_3179 at 3785.173 kb on - strand, within Shew_3179 at 3785.262 kb on + strand, within Shew_3179 at 3785.586 kb on + strand at 3785.589 kb on - strand at 3785.858 kb on + strand, within Shew_3180 at 3786.127 kb on + strand, within Shew_3180 at 3786.137 kb on - strand, within Shew_3180 at 3786.160 kb on + strand, within Shew_3180 at 3786.207 kb on - strand at 3786.214 kb on + strand at 3786.232 kb on - strand at 3786.345 kb on + strand at 3786.611 kb on - strand at 3786.957 kb on - strand at 3787.042 kb on + strand, within Shew_3181 at 3787.073 kb on - strand, within Shew_3181 at 3787.123 kb on + strand, within Shew_3181
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 3,784,573 + +0.1 3,784,610 - +0.2 3,784,650 - +1.8 3,784,652 + +0.1 3,784,660 + -0.6 3,784,835 - Shew_3179 0.12 -0.9 3,784,900 - Shew_3179 0.20 -0.3 3,785,057 + Shew_3179 0.40 -0.1 3,785,173 - Shew_3179 0.55 -0.2 3,785,262 + Shew_3179 0.67 -0.2 3,785,586 + +0.4 3,785,589 - +0.5 3,785,858 + Shew_3180 0.46 -0.3 3,786,127 + Shew_3180 0.83 -0.3 3,786,137 - Shew_3180 0.85 +0.2 3,786,160 + Shew_3180 0.88 +0.0 3,786,207 - -0.1 3,786,214 + -0.4 3,786,232 - +0.4 3,786,345 + -0.0 3,786,611 - -0.3 3,786,957 - +0.4 3,787,042 + Shew_3181 0.15 +0.0 3,787,073 - Shew_3181 0.16 +0.5 3,787,123 + Shew_3181 0.19 +0.2
Or see this region's nucleotide sequence