Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3070 and Shew_3071 are separated by 357 nucleotides Shew_3071 and Shew_3072 are separated by 15 nucleotides Shew_3072 and Shew_3073 are separated by 182 nucleotides Shew_3073 and Shew_3074 are separated by 133 nucleotides
Shew_3070: Shew_3070 - extracellular solute-binding protein (RefSeq), at 3,645,607 to 3,646,467
_3070
Shew_3071: Shew_3071 - lipoprotein (RefSeq), at 3,646,825 to 3,647,334
_3071
Shew_3072: Shew_3072 - cold-shock protein, DNA-binding (RefSeq), at 3,647,350 to 3,647,943
_3072
Shew_3073: Shew_3073 - uracil-DNA glycosylase (RefSeq), at 3,648,126 to 3,648,794
_3073
Shew_3074: Shew_3074 - pilin, putative (RefSeq), at 3,648,928 to 3,649,464
_3074
Position (kb)
3647
3648 Strain fitness (log2 ratio)
-2
-1
0
1 at 3646.461 kb on - strand at 3646.531 kb on + strand at 3646.531 kb on + strand at 3646.714 kb on - strand at 3646.770 kb on + strand at 3647.017 kb on + strand, within Shew_3071 at 3647.030 kb on - strand, within Shew_3071 at 3647.214 kb on - strand, within Shew_3071 at 3647.228 kb on - strand, within Shew_3071 at 3647.286 kb on + strand at 3647.286 kb on + strand at 3647.883 kb on + strand, within Shew_3072 at 3647.891 kb on - strand at 3647.948 kb on - strand at 3648.004 kb on - strand at 3648.282 kb on - strand, within Shew_3073 at 3648.446 kb on + strand, within Shew_3073 at 3648.622 kb on + strand, within Shew_3073 at 3648.630 kb on - strand, within Shew_3073 at 3648.630 kb on - strand, within Shew_3073 at 3648.631 kb on - strand, within Shew_3073 at 3648.636 kb on - strand, within Shew_3073 at 3648.663 kb on - strand, within Shew_3073 at 3648.912 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 3,646,461 - +0.4 3,646,531 + -0.5 3,646,531 + -0.6 3,646,714 - -0.0 3,646,770 + +0.7 3,647,017 + Shew_3071 0.38 +1.2 3,647,030 - Shew_3071 0.40 +1.7 3,647,214 - Shew_3071 0.76 +0.1 3,647,228 - Shew_3071 0.79 +0.0 3,647,286 + +0.2 3,647,286 + +0.2 3,647,883 + Shew_3072 0.90 -1.0 3,647,891 - +0.2 3,647,948 - -1.8 3,648,004 - +0.4 3,648,282 - Shew_3073 0.23 -0.1 3,648,446 + Shew_3073 0.48 -0.2 3,648,622 + Shew_3073 0.74 +0.4 3,648,630 - Shew_3073 0.75 -0.1 3,648,630 - Shew_3073 0.75 +0.1 3,648,631 - Shew_3073 0.75 -0.1 3,648,636 - Shew_3073 0.76 +0.6 3,648,663 - Shew_3073 0.80 +0.7 3,648,912 + +0.2
Or see this region's nucleotide sequence