Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2615 and Shew_2616 are separated by 20 nucleotides Shew_2616 and Shew_2617 are separated by 70 nucleotides Shew_2617 and Shew_2618 are separated by 299 nucleotides
Shew_2615: Shew_2615 - patatin (RefSeq), at 3,085,347 to 3,086,471
_2615
Shew_2616: Shew_2616 - GDSL family lipase (RefSeq), at 3,086,492 to 3,087,169
_2616
Shew_2617: Shew_2617 - potassium efflux system protein (RefSeq), at 3,087,240 to 3,089,189
_2617
Shew_2618: Shew_2618 - diguanylate cyclase (RefSeq), at 3,089,489 to 3,090,376
_2618
Position (kb)
3087
3088
3089
3090 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3086.282 kb on + strand, within Shew_2615 at 3086.832 kb on - strand, within Shew_2616 at 3087.133 kb on + strand at 3087.141 kb on - strand at 3087.229 kb on + strand at 3087.378 kb on - strand at 3087.735 kb on - strand, within Shew_2617 at 3088.433 kb on + strand, within Shew_2617 at 3088.720 kb on + strand, within Shew_2617 at 3088.839 kb on - strand, within Shew_2617 at 3089.636 kb on + strand, within Shew_2618 at 3089.663 kb on + strand, within Shew_2618 at 3089.943 kb on + strand, within Shew_2618 at 3089.951 kb on - strand, within Shew_2618 at 3089.955 kb on + strand, within Shew_2618
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 3,086,282 + Shew_2615 0.83 -0.3 3,086,832 - Shew_2616 0.50 +0.3 3,087,133 + +0.8 3,087,141 - +0.0 3,087,229 + +0.2 3,087,378 - +0.1 3,087,735 - Shew_2617 0.25 -0.3 3,088,433 + Shew_2617 0.61 -0.7 3,088,720 + Shew_2617 0.76 -2.1 3,088,839 - Shew_2617 0.82 -0.0 3,089,636 + Shew_2618 0.17 +0.1 3,089,663 + Shew_2618 0.20 +0.1 3,089,943 + Shew_2618 0.51 -0.4 3,089,951 - Shew_2618 0.52 +0.6 3,089,955 + Shew_2618 0.52 +1.8
Or see this region's nucleotide sequence