Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1891 and Shew_1892 are separated by 174 nucleotides Shew_1892 and Shew_1893 are separated by 58 nucleotides Shew_1893 and Shew_1894 are separated by 226 nucleotides
Shew_1891: Shew_1891 - Fmu (Sun) domain-containing protein (RefSeq), at 2,182,111 to 2,183,367
_1891
Shew_1892: Shew_1892 - SNF2-related protein (RefSeq), at 2,183,542 to 2,187,027
_1892
Shew_1893: Shew_1893 - hypothetical protein (RefSeq), at 2,187,086 to 2,187,505
_1893
Shew_1894: Shew_1894 - hypothetical protein (RefSeq), at 2,187,732 to 2,189,663
_1894
Position (kb)
2183
2184
2185
2186
2187
2188 Strain fitness (log2 ratio)
-2
-1
0
1 at 2182.855 kb on + strand, within Shew_1891 at 2182.948 kb on - strand, within Shew_1891 at 2183.932 kb on + strand, within Shew_1892 at 2184.079 kb on - strand, within Shew_1892 at 2184.232 kb on + strand, within Shew_1892 at 2185.522 kb on + strand, within Shew_1892 at 2185.614 kb on + strand, within Shew_1892 at 2185.622 kb on - strand, within Shew_1892 at 2185.900 kb on + strand, within Shew_1892 at 2187.083 kb on + strand at 2187.148 kb on + strand, within Shew_1893 at 2187.270 kb on + strand, within Shew_1893 at 2187.292 kb on + strand, within Shew_1893 at 2187.331 kb on + strand, within Shew_1893 at 2187.462 kb on - strand, within Shew_1893 at 2187.805 kb on + strand at 2187.927 kb on + strand, within Shew_1894
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 2,182,855 + Shew_1891 0.59 -1.6 2,182,948 - Shew_1891 0.67 +0.6 2,183,932 + Shew_1892 0.11 -0.3 2,184,079 - Shew_1892 0.15 -0.1 2,184,232 + Shew_1892 0.20 -1.2 2,185,522 + Shew_1892 0.57 -0.1 2,185,614 + Shew_1892 0.59 -0.8 2,185,622 - Shew_1892 0.60 -0.3 2,185,900 + Shew_1892 0.68 -1.7 2,187,083 + +0.2 2,187,148 + Shew_1893 0.15 -1.9 2,187,270 + Shew_1893 0.44 +0.5 2,187,292 + Shew_1893 0.49 -0.0 2,187,331 + Shew_1893 0.58 -1.3 2,187,462 - Shew_1893 0.90 +0.0 2,187,805 + +1.0 2,187,927 + Shew_1894 0.10 -0.2
Or see this region's nucleotide sequence