Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0829 and Shew_0830 are separated by 13 nucleotides Shew_0830 and Shew_0831 are separated by 40 nucleotides Shew_0831 and Shew_0832 are separated by 358 nucleotides
Shew_0829: Shew_0829 - LrgA family protein (RefSeq), at 951,706 to 952,125
_0829
Shew_0830: Shew_0830 - LrgB family protein (RefSeq), at 952,139 to 952,828
_0830
Shew_0831: Shew_0831 - CBS domain-containing protein (RefSeq), at 952,869 to 953,288
_0831
Shew_0832: Shew_0832 - alkaline phosphatase (RefSeq), at 953,647 to 955,419
_0832
Position (kb) 952
953
954 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 951.987 kb on + strand, within Shew_0829 at 952.010 kb on + strand, within Shew_0829 at 952.010 kb on - strand, within Shew_0829 at 952.018 kb on - strand, within Shew_0829 at 952.196 kb on - strand at 952.196 kb on - strand at 952.435 kb on - strand, within Shew_0830 at 952.501 kb on + strand, within Shew_0830 at 952.763 kb on + strand at 952.903 kb on - strand at 952.903 kb on - strand at 953.524 kb on + strand at 953.605 kb on - strand at 953.942 kb on - strand, within Shew_0832 at 954.027 kb on + strand, within Shew_0832
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 951,987 + Shew_0829 0.67 -0.4 952,010 + Shew_0829 0.72 -0.1 952,010 - Shew_0829 0.72 -0.3 952,018 - Shew_0829 0.74 -0.1 952,196 - +1.1 952,196 - +0.4 952,435 - Shew_0830 0.43 -0.1 952,501 + Shew_0830 0.52 +0.2 952,763 + +0.2 952,903 - +0.1 952,903 - +0.0 953,524 + -0.1 953,605 - +3.8 953,942 - Shew_0832 0.17 +0.3 954,027 + Shew_0832 0.21 -0.0
Or see this region's nucleotide sequence