Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0454 and Shew_0455 are separated by 165 nucleotides Shew_0455 and Shew_0456 are separated by 47 nucleotides Shew_0456 and Shew_0457 are separated by 135 nucleotides
Shew_0454: Shew_0454 - enoyl-CoA hydratase (RefSeq), at 504,816 to 505,625
_0454
Shew_0455: Shew_0455 - hypothetical protein (RefSeq), at 505,791 to 506,294
_0455
Shew_0456: Shew_0456 - hypothetical protein (RefSeq), at 506,342 to 507,226
_0456
Shew_0457: Shew_0457 - ATP-dependent helicase HepA (RefSeq), at 507,362 to 510,268
_0457
Position (kb) 506
507
508 Strain fitness (log2 ratio) -1
0
1 at 506.038 kb on + strand, within Shew_0455 at 506.209 kb on - strand, within Shew_0455 at 506.383 kb on - strand at 506.406 kb on + strand at 506.542 kb on - strand, within Shew_0456 at 506.561 kb on - strand, within Shew_0456 at 506.617 kb on - strand, within Shew_0456 at 506.621 kb on + strand, within Shew_0456 at 506.634 kb on - strand, within Shew_0456 at 506.746 kb on + strand, within Shew_0456 at 506.861 kb on + strand, within Shew_0456 at 506.919 kb on - strand, within Shew_0456 at 506.933 kb on + strand, within Shew_0456 at 507.040 kb on + strand, within Shew_0456 at 507.079 kb on + strand, within Shew_0456 at 507.137 kb on - strand, within Shew_0456 at 507.142 kb on - strand at 507.192 kb on + strand at 507.277 kb on - strand at 507.517 kb on + strand at 507.633 kb on + strand at 507.910 kb on - strand, within Shew_0457 at 508.009 kb on + strand, within Shew_0457 at 508.059 kb on - strand, within Shew_0457 at 508.106 kb on + strand, within Shew_0457 at 508.207 kb on + strand, within Shew_0457
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 506,038 + Shew_0455 0.49 +0.1 506,209 - Shew_0455 0.83 +0.4 506,383 - -0.4 506,406 + -0.1 506,542 - Shew_0456 0.23 -0.6 506,561 - Shew_0456 0.25 +0.0 506,617 - Shew_0456 0.31 -0.4 506,621 + Shew_0456 0.32 -0.1 506,634 - Shew_0456 0.33 -0.4 506,746 + Shew_0456 0.46 -0.4 506,861 + Shew_0456 0.59 -0.9 506,919 - Shew_0456 0.65 +0.1 506,933 + Shew_0456 0.67 -0.4 507,040 + Shew_0456 0.79 -0.0 507,079 + Shew_0456 0.83 +0.1 507,137 - Shew_0456 0.90 +0.0 507,142 - -0.0 507,192 + -0.1 507,277 - -0.4 507,517 + -0.1 507,633 + +0.0 507,910 - Shew_0457 0.19 -0.1 508,009 + Shew_0457 0.22 -0.2 508,059 - Shew_0457 0.24 -0.3 508,106 + Shew_0457 0.26 -0.1 508,207 + Shew_0457 0.29 -0.5
Or see this region's nucleotide sequence