Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0310 and Shew_0311 are separated by 31 nucleotides Shew_0311 and Shew_0312 overlap by 8 nucleotides
Shew_0310: Shew_0310 - TolC family type I secretion outer membrane protein (RefSeq), at 335,861 to 337,273
_0310
Shew_0311: Shew_0311 - HlyD family type I secretion membrane fusion protein (RefSeq), at 337,305 to 338,687
_0311
Shew_0312: Shew_0312 - ABC transporter-related protein (RefSeq), at 338,680 to 340,860
_0312
Position (kb) 337
338
339 Strain fitness (log2 ratio) -1
0
1 at 336.414 kb on + strand, within Shew_0310 at 336.651 kb on - strand, within Shew_0310 at 336.711 kb on - strand, within Shew_0310 at 336.804 kb on + strand, within Shew_0310 at 337.000 kb on - strand, within Shew_0310 at 337.000 kb on - strand, within Shew_0310 at 337.017 kb on + strand, within Shew_0310 at 337.182 kb on - strand at 337.462 kb on + strand, within Shew_0311 at 337.470 kb on - strand, within Shew_0311 at 337.524 kb on - strand, within Shew_0311 at 337.891 kb on + strand, within Shew_0311 at 337.901 kb on + strand, within Shew_0311 at 338.093 kb on - strand, within Shew_0311 at 338.192 kb on + strand, within Shew_0311 at 338.251 kb on + strand, within Shew_0311 at 338.251 kb on + strand, within Shew_0311 at 338.331 kb on + strand, within Shew_0311 at 338.497 kb on + strand, within Shew_0311 at 338.595 kb on - strand at 338.679 kb on - strand at 338.695 kb on - strand at 338.707 kb on - strand at 338.724 kb on + strand at 338.945 kb on + strand, within Shew_0312 at 339.066 kb on - strand, within Shew_0312 at 339.201 kb on - strand, within Shew_0312 at 339.270 kb on + strand, within Shew_0312 at 339.278 kb on - strand, within Shew_0312 at 339.358 kb on + strand, within Shew_0312 at 339.488 kb on - strand, within Shew_0312
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 336,414 + Shew_0310 0.39 +0.1 336,651 - Shew_0310 0.56 +0.3 336,711 - Shew_0310 0.60 +0.1 336,804 + Shew_0310 0.67 +0.1 337,000 - Shew_0310 0.81 -0.1 337,000 - Shew_0310 0.81 +0.2 337,017 + Shew_0310 0.82 -0.1 337,182 - +0.6 337,462 + Shew_0311 0.11 -0.8 337,470 - Shew_0311 0.12 -0.7 337,524 - Shew_0311 0.16 -0.1 337,891 + Shew_0311 0.42 -0.7 337,901 + Shew_0311 0.43 -0.3 338,093 - Shew_0311 0.57 -0.2 338,192 + Shew_0311 0.64 -0.2 338,251 + Shew_0311 0.68 -0.3 338,251 + Shew_0311 0.68 -0.6 338,331 + Shew_0311 0.74 +0.3 338,497 + Shew_0311 0.86 +0.1 338,595 - +0.5 338,679 - -0.6 338,695 - -0.1 338,707 - +0.2 338,724 + +0.8 338,945 + Shew_0312 0.12 +1.0 339,066 - Shew_0312 0.18 +0.1 339,201 - Shew_0312 0.24 +0.6 339,270 + Shew_0312 0.27 +0.7 339,278 - Shew_0312 0.27 +0.4 339,358 + Shew_0312 0.31 +0.2 339,488 - Shew_0312 0.37 +0.3
Or see this region's nucleotide sequence