Experiment: Spectinomycin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0020 and Shew_0021 are separated by 14 nucleotides Shew_0021 and Shew_0022 are separated by 152 nucleotides
Shew_0020: Shew_0020 - proline dipeptidase (RefSeq), at 23,495 to 24,817
_0020
Shew_0021: Shew_0021 - hypothetical protein (RefSeq), at 24,832 to 25,446
_0021
Shew_0022: Shew_0022 - TrkH family potassium uptake protein (RefSeq), at 25,599 to 27,056
_0022
Position (kb)
24
25
26 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 23.937 kb on - strand, within Shew_0020 at 23.986 kb on - strand, within Shew_0020 at 24.110 kb on + strand, within Shew_0020 at 24.172 kb on - strand, within Shew_0020 at 24.177 kb on - strand, within Shew_0020 at 24.321 kb on + strand, within Shew_0020 at 24.329 kb on - strand, within Shew_0020 at 24.374 kb on - strand, within Shew_0020 at 24.428 kb on + strand, within Shew_0020 at 24.449 kb on - strand, within Shew_0020 at 24.475 kb on - strand, within Shew_0020 at 24.566 kb on - strand, within Shew_0020 at 24.811 kb on - strand at 25.148 kb on + strand, within Shew_0021 at 25.223 kb on - strand, within Shew_0021 at 25.227 kb on + strand, within Shew_0021 at 25.283 kb on - strand, within Shew_0021 at 25.307 kb on + strand, within Shew_0021 at 25.365 kb on + strand, within Shew_0021 at 25.375 kb on + strand, within Shew_0021 at 25.375 kb on + strand, within Shew_0021 at 25.418 kb on - strand at 25.462 kb on - strand at 25.795 kb on + strand, within Shew_0022 at 25.823 kb on + strand, within Shew_0022 at 25.823 kb on + strand, within Shew_0022 at 25.933 kb on - strand, within Shew_0022 at 25.967 kb on + strand, within Shew_0022 at 26.182 kb on - strand, within Shew_0022
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 10 mg/ml remove 23,937 - Shew_0020 0.33 +0.3 23,986 - Shew_0020 0.37 -0.0 24,110 + Shew_0020 0.46 +0.0 24,172 - Shew_0020 0.51 +0.7 24,177 - Shew_0020 0.52 +2.1 24,321 + Shew_0020 0.62 +0.1 24,329 - Shew_0020 0.63 -0.1 24,374 - Shew_0020 0.66 +0.1 24,428 + Shew_0020 0.71 -0.2 24,449 - Shew_0020 0.72 +0.1 24,475 - Shew_0020 0.74 +0.1 24,566 - Shew_0020 0.81 -0.6 24,811 - +0.0 25,148 + Shew_0021 0.51 -0.5 25,223 - Shew_0021 0.64 +0.1 25,227 + Shew_0021 0.64 -1.4 25,283 - Shew_0021 0.73 -0.6 25,307 + Shew_0021 0.77 +0.1 25,365 + Shew_0021 0.87 -2.0 25,375 + Shew_0021 0.88 +0.5 25,375 + Shew_0021 0.88 -0.7 25,418 - -1.2 25,462 - -0.6 25,795 + Shew_0022 0.13 +0.6 25,823 + Shew_0022 0.15 +0.4 25,823 + Shew_0022 0.15 +0.6 25,933 - Shew_0022 0.23 +0.8 25,967 + Shew_0022 0.25 +0.5 26,182 - Shew_0022 0.40 +0.7
Or see this region's nucleotide sequence