Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_940 and Pf6N2E2_941 are separated by 81 nucleotides Pf6N2E2_941 and Pf6N2E2_942 are separated by 197 nucleotides Pf6N2E2_942 and Pf6N2E2_943 are separated by 213 nucleotides
Pf6N2E2_940 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 1,107,183 to 1,108,211
_940
Pf6N2E2_941 - Probable transmembrane protein, at 1,108,293 to 1,108,925
_941
Pf6N2E2_942 - Mll6465 protein, at 1,109,123 to 1,109,614
_942
Pf6N2E2_943 - Transcriptional regulator, AraC family, at 1,109,828 to 1,110,874
_943
Position (kb)
1109
1110 Strain fitness (log2 ratio)
-2
-1
0
1 at 1108.173 kb on + strand at 1108.173 kb on + strand at 1108.209 kb on + strand at 1108.209 kb on + strand at 1108.210 kb on - strand at 1108.247 kb on + strand at 1108.289 kb on + strand at 1108.290 kb on - strand at 1108.418 kb on + strand, within Pf6N2E2_941 at 1108.419 kb on - strand, within Pf6N2E2_941 at 1108.419 kb on - strand, within Pf6N2E2_941 at 1108.419 kb on - strand, within Pf6N2E2_941 at 1108.419 kb on - strand, within Pf6N2E2_941 at 1108.435 kb on - strand, within Pf6N2E2_941 at 1108.479 kb on - strand, within Pf6N2E2_941 at 1108.479 kb on - strand, within Pf6N2E2_941 at 1108.631 kb on + strand, within Pf6N2E2_941 at 1108.714 kb on + strand, within Pf6N2E2_941 at 1108.766 kb on - strand, within Pf6N2E2_941 at 1108.766 kb on - strand, within Pf6N2E2_941 at 1108.812 kb on - strand, within Pf6N2E2_941 at 1108.812 kb on - strand, within Pf6N2E2_941 at 1108.888 kb on + strand at 1108.888 kb on + strand at 1108.888 kb on + strand at 1108.888 kb on + strand at 1108.888 kb on + strand at 1108.888 kb on + strand at 1108.889 kb on - strand at 1108.889 kb on - strand at 1108.941 kb on - strand at 1108.963 kb on - strand at 1109.046 kb on + strand at 1109.046 kb on + strand at 1109.046 kb on + strand at 1109.047 kb on - strand at 1109.059 kb on - strand at 1109.158 kb on + strand at 1109.159 kb on - strand at 1109.159 kb on - strand at 1109.164 kb on + strand at 1109.164 kb on + strand at 1109.538 kb on - strand, within Pf6N2E2_942 at 1109.538 kb on - strand, within Pf6N2E2_942 at 1109.628 kb on + strand at 1109.777 kb on + strand at 1109.777 kb on + strand at 1109.777 kb on + strand at 1109.778 kb on - strand at 1109.848 kb on + strand at 1109.848 kb on + strand at 1109.849 kb on - strand at 1109.943 kb on + strand, within Pf6N2E2_943 at 1109.943 kb on + strand, within Pf6N2E2_943 at 1109.944 kb on - strand, within Pf6N2E2_943 at 1109.977 kb on - strand, within Pf6N2E2_943 at 1110.011 kb on + strand, within Pf6N2E2_943 at 1110.119 kb on - strand, within Pf6N2E2_943 at 1110.126 kb on + strand, within Pf6N2E2_943 at 1110.127 kb on - strand, within Pf6N2E2_943 at 1110.473 kb on + strand, within Pf6N2E2_943 at 1110.474 kb on - strand, within Pf6N2E2_943
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,108,173 + -1.3 1,108,173 + +0.1 1,108,209 + -0.6 1,108,209 + -0.4 1,108,210 - -1.1 1,108,247 + +0.6 1,108,289 + +0.1 1,108,290 - -0.6 1,108,418 + Pf6N2E2_941 0.20 -0.5 1,108,419 - Pf6N2E2_941 0.20 -1.1 1,108,419 - Pf6N2E2_941 0.20 +1.6 1,108,419 - Pf6N2E2_941 0.20 -0.3 1,108,419 - Pf6N2E2_941 0.20 +0.8 1,108,435 - Pf6N2E2_941 0.22 -0.2 1,108,479 - Pf6N2E2_941 0.29 -0.2 1,108,479 - Pf6N2E2_941 0.29 -0.3 1,108,631 + Pf6N2E2_941 0.53 +0.3 1,108,714 + Pf6N2E2_941 0.67 -1.2 1,108,766 - Pf6N2E2_941 0.75 -0.4 1,108,766 - Pf6N2E2_941 0.75 -0.5 1,108,812 - Pf6N2E2_941 0.82 -0.7 1,108,812 - Pf6N2E2_941 0.82 +0.4 1,108,888 + +0.7 1,108,888 + -1.9 1,108,888 + -1.8 1,108,888 + -0.3 1,108,888 + -1.2 1,108,888 + -1.5 1,108,889 - -0.6 1,108,889 - -1.1 1,108,941 - -0.5 1,108,963 - +1.0 1,109,046 + -0.2 1,109,046 + +1.1 1,109,046 + -0.2 1,109,047 - -1.2 1,109,059 - -0.5 1,109,158 + +0.0 1,109,159 - +0.3 1,109,159 - -1.2 1,109,164 + -0.0 1,109,164 + +0.8 1,109,538 - Pf6N2E2_942 0.84 -1.3 1,109,538 - Pf6N2E2_942 0.84 -1.1 1,109,628 + +0.2 1,109,777 + -0.5 1,109,777 + -0.8 1,109,777 + +0.8 1,109,778 - +0.9 1,109,848 + -1.3 1,109,848 + -0.7 1,109,849 - -2.1 1,109,943 + Pf6N2E2_943 0.11 +0.2 1,109,943 + Pf6N2E2_943 0.11 +0.6 1,109,944 - Pf6N2E2_943 0.11 -1.7 1,109,977 - Pf6N2E2_943 0.14 -0.2 1,110,011 + Pf6N2E2_943 0.17 -0.5 1,110,119 - Pf6N2E2_943 0.28 +1.0 1,110,126 + Pf6N2E2_943 0.28 -0.5 1,110,127 - Pf6N2E2_943 0.29 +0.8 1,110,473 + Pf6N2E2_943 0.62 -1.7 1,110,474 - Pf6N2E2_943 0.62 +0.4
Or see this region's nucleotide sequence