Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_815 and Pf6N2E2_816 overlap by 1 nucleotides Pf6N2E2_816 and Pf6N2E2_817 overlap by 4 nucleotides Pf6N2E2_817 and Pf6N2E2_818 are separated by 5 nucleotides
Pf6N2E2_815 - Signal transduction histidine kinase, at 946,888 to 950,370
_815
Pf6N2E2_816 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 950,370 to 951,185
_816
Pf6N2E2_817 - Protein-glutamate methylesterase (EC 3.1.1.61), at 951,182 to 951,769
_817
Pf6N2E2_818 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 951,775 to 953,055
_818
Position (kb)
950
951
952 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 949.793 kb on - strand, within Pf6N2E2_815 at 949.840 kb on + strand, within Pf6N2E2_815 at 949.841 kb on - strand, within Pf6N2E2_815 at 949.841 kb on - strand, within Pf6N2E2_815 at 950.150 kb on + strand at 950.150 kb on + strand at 950.150 kb on + strand at 950.150 kb on + strand at 950.150 kb on + strand at 950.151 kb on - strand at 950.151 kb on - strand at 950.151 kb on - strand at 950.151 kb on - strand at 950.151 kb on - strand at 950.227 kb on - strand at 950.427 kb on + strand at 950.427 kb on + strand at 950.428 kb on - strand at 950.428 kb on - strand at 950.433 kb on + strand at 950.658 kb on + strand, within Pf6N2E2_816 at 950.659 kb on - strand, within Pf6N2E2_816 at 950.709 kb on + strand at 950.710 kb on - strand, within Pf6N2E2_816 at 950.764 kb on - strand, within Pf6N2E2_816 at 950.835 kb on - strand, within Pf6N2E2_816 at 950.835 kb on - strand, within Pf6N2E2_816 at 950.835 kb on - strand, within Pf6N2E2_816 at 950.835 kb on - strand, within Pf6N2E2_816 at 950.839 kb on - strand, within Pf6N2E2_816 at 950.839 kb on - strand, within Pf6N2E2_816 at 950.839 kb on - strand, within Pf6N2E2_816 at 950.839 kb on - strand, within Pf6N2E2_816 at 950.839 kb on - strand, within Pf6N2E2_816 at 950.839 kb on - strand, within Pf6N2E2_816 at 950.887 kb on - strand, within Pf6N2E2_816 at 950.887 kb on - strand, within Pf6N2E2_816 at 950.887 kb on - strand, within Pf6N2E2_816 at 950.890 kb on - strand, within Pf6N2E2_816 at 950.890 kb on - strand, within Pf6N2E2_816 at 950.890 kb on - strand, within Pf6N2E2_816 at 950.890 kb on - strand, within Pf6N2E2_816 at 950.890 kb on - strand, within Pf6N2E2_816 at 950.890 kb on - strand, within Pf6N2E2_816 at 950.890 kb on - strand, within Pf6N2E2_816 at 950.890 kb on - strand, within Pf6N2E2_816 at 951.019 kb on + strand at 951.022 kb on - strand, within Pf6N2E2_816 at 951.022 kb on - strand, within Pf6N2E2_816 at 951.022 kb on - strand, within Pf6N2E2_816 at 951.022 kb on - strand, within Pf6N2E2_816 at 951.315 kb on + strand, within Pf6N2E2_817 at 951.316 kb on - strand, within Pf6N2E2_817 at 951.426 kb on + strand, within Pf6N2E2_817 at 951.426 kb on + strand, within Pf6N2E2_817 at 951.426 kb on + strand, within Pf6N2E2_817 at 951.426 kb on + strand, within Pf6N2E2_817 at 951.426 kb on + strand, within Pf6N2E2_817 at 951.427 kb on - strand, within Pf6N2E2_817 at 951.427 kb on - strand, within Pf6N2E2_817 at 951.427 kb on - strand, within Pf6N2E2_817 at 951.427 kb on - strand, within Pf6N2E2_817 at 951.427 kb on - strand, within Pf6N2E2_817 at 951.427 kb on - strand, within Pf6N2E2_817 at 951.427 kb on - strand, within Pf6N2E2_817 at 951.428 kb on + strand, within Pf6N2E2_817 at 951.428 kb on + strand, within Pf6N2E2_817 at 951.428 kb on + strand, within Pf6N2E2_817 at 951.428 kb on + strand, within Pf6N2E2_817 at 951.428 kb on + strand, within Pf6N2E2_817 at 951.429 kb on - strand, within Pf6N2E2_817 at 951.429 kb on - strand, within Pf6N2E2_817 at 951.429 kb on - strand, within Pf6N2E2_817 at 951.429 kb on - strand, within Pf6N2E2_817 at 951.429 kb on - strand, within Pf6N2E2_817 at 951.429 kb on - strand, within Pf6N2E2_817 at 951.429 kb on - strand, within Pf6N2E2_817 at 951.721 kb on + strand at 951.727 kb on + strand at 951.727 kb on + strand at 951.727 kb on + strand at 951.728 kb on - strand at 951.786 kb on + strand at 951.786 kb on + strand at 951.787 kb on - strand at 951.787 kb on - strand at 951.787 kb on - strand at 952.021 kb on - strand, within Pf6N2E2_818 at 952.057 kb on + strand, within Pf6N2E2_818 at 952.057 kb on + strand, within Pf6N2E2_818 at 952.057 kb on + strand, within Pf6N2E2_818 at 952.057 kb on + strand, within Pf6N2E2_818 at 952.058 kb on - strand, within Pf6N2E2_818 at 952.058 kb on - strand, within Pf6N2E2_818 at 952.058 kb on - strand, within Pf6N2E2_818 at 952.156 kb on + strand, within Pf6N2E2_818 at 952.157 kb on - strand, within Pf6N2E2_818 at 952.157 kb on - strand, within Pf6N2E2_818 at 952.157 kb on - strand, within Pf6N2E2_818
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 949,793 - Pf6N2E2_815 0.83 +1.8 949,840 + Pf6N2E2_815 0.85 -1.3 949,841 - Pf6N2E2_815 0.85 +3.3 949,841 - Pf6N2E2_815 0.85 +4.0 950,150 + -3.0 950,150 + -2.7 950,150 + -0.4 950,150 + +1.2 950,150 + +0.0 950,151 - +1.9 950,151 - +2.0 950,151 - +3.0 950,151 - +2.1 950,151 - +3.8 950,227 - +1.3 950,427 + -0.9 950,427 + -2.7 950,428 - +0.0 950,428 - +1.9 950,433 + +0.4 950,658 + Pf6N2E2_816 0.35 -3.6 950,659 - Pf6N2E2_816 0.35 -0.2 950,709 + +0.4 950,710 - Pf6N2E2_816 0.42 +1.3 950,764 - Pf6N2E2_816 0.48 -1.6 950,835 - Pf6N2E2_816 0.57 +0.7 950,835 - Pf6N2E2_816 0.57 +0.3 950,835 - Pf6N2E2_816 0.57 +2.0 950,835 - Pf6N2E2_816 0.57 +1.9 950,839 - Pf6N2E2_816 0.57 +1.2 950,839 - Pf6N2E2_816 0.57 +3.8 950,839 - Pf6N2E2_816 0.57 +2.1 950,839 - Pf6N2E2_816 0.57 +1.6 950,839 - Pf6N2E2_816 0.57 +0.5 950,839 - Pf6N2E2_816 0.57 +1.5 950,887 - Pf6N2E2_816 0.63 +1.9 950,887 - Pf6N2E2_816 0.63 +2.5 950,887 - Pf6N2E2_816 0.63 +1.0 950,890 - Pf6N2E2_816 0.64 +3.0 950,890 - Pf6N2E2_816 0.64 -0.8 950,890 - Pf6N2E2_816 0.64 +1.5 950,890 - Pf6N2E2_816 0.64 +1.8 950,890 - Pf6N2E2_816 0.64 +3.2 950,890 - Pf6N2E2_816 0.64 +2.2 950,890 - Pf6N2E2_816 0.64 +2.3 950,890 - Pf6N2E2_816 0.64 -2.0 951,019 + +0.7 951,022 - Pf6N2E2_816 0.80 +2.7 951,022 - Pf6N2E2_816 0.80 -2.3 951,022 - Pf6N2E2_816 0.80 +2.3 951,022 - Pf6N2E2_816 0.80 +0.9 951,315 + Pf6N2E2_817 0.23 +0.7 951,316 - Pf6N2E2_817 0.23 +0.1 951,426 + Pf6N2E2_817 0.41 -1.0 951,426 + Pf6N2E2_817 0.41 -1.4 951,426 + Pf6N2E2_817 0.41 -0.9 951,426 + Pf6N2E2_817 0.41 -1.7 951,426 + Pf6N2E2_817 0.41 -2.1 951,427 - Pf6N2E2_817 0.42 +1.4 951,427 - Pf6N2E2_817 0.42 +2.0 951,427 - Pf6N2E2_817 0.42 +3.0 951,427 - Pf6N2E2_817 0.42 +1.9 951,427 - Pf6N2E2_817 0.42 +1.8 951,427 - Pf6N2E2_817 0.42 +3.2 951,427 - Pf6N2E2_817 0.42 +3.8 951,428 + Pf6N2E2_817 0.42 -1.4 951,428 + Pf6N2E2_817 0.42 +1.0 951,428 + Pf6N2E2_817 0.42 -0.3 951,428 + Pf6N2E2_817 0.42 -1.9 951,428 + Pf6N2E2_817 0.42 -1.7 951,429 - Pf6N2E2_817 0.42 +1.9 951,429 - Pf6N2E2_817 0.42 +2.6 951,429 - Pf6N2E2_817 0.42 +1.0 951,429 - Pf6N2E2_817 0.42 +0.8 951,429 - Pf6N2E2_817 0.42 -2.2 951,429 - Pf6N2E2_817 0.42 +3.7 951,429 - Pf6N2E2_817 0.42 +1.8 951,721 + +1.4 951,727 + -1.2 951,727 + -1.9 951,727 + -2.7 951,728 - +0.7 951,786 + +2.0 951,786 + +2.2 951,787 - +2.2 951,787 - +3.2 951,787 - +2.5 952,021 - Pf6N2E2_818 0.19 +2.0 952,057 + Pf6N2E2_818 0.22 +2.5 952,057 + Pf6N2E2_818 0.22 +3.6 952,057 + Pf6N2E2_818 0.22 +1.5 952,057 + Pf6N2E2_818 0.22 +2.0 952,058 - Pf6N2E2_818 0.22 +1.8 952,058 - Pf6N2E2_818 0.22 -3.0 952,058 - Pf6N2E2_818 0.22 +3.4 952,156 + Pf6N2E2_818 0.30 +2.9 952,157 - Pf6N2E2_818 0.30 +2.3 952,157 - Pf6N2E2_818 0.30 +1.2 952,157 - Pf6N2E2_818 0.30 +3.6
Or see this region's nucleotide sequence