Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_784

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_783 and Pf6N2E2_784 are separated by 61 nucleotidesPf6N2E2_784 and Pf6N2E2_785 are separated by 37 nucleotidesPf6N2E2_785 and Pf6N2E2_786 are separated by 109 nucleotides Pf6N2E2_783 - hypothetical protein, at 896,431 to 897,528 _783 Pf6N2E2_784 - Outer membrane usher protein FIMD, at 897,590 to 900,085 _784 Pf6N2E2_785 - P pilus assembly protein, chaperone PapD, at 900,123 to 900,884 _785 Pf6N2E2_786 - Type-1 fimbrial protein, A chain precursor, at 900,994 to 901,542 _786 Position (kb) 897 898 899 900 901Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 896.614 kb on + strand, within Pf6N2E2_783at 896.614 kb on + strand, within Pf6N2E2_783at 896.615 kb on - strand, within Pf6N2E2_783at 896.763 kb on + strand, within Pf6N2E2_783at 896.763 kb on + strand, within Pf6N2E2_783at 896.763 kb on + strand, within Pf6N2E2_783at 896.763 kb on + strand, within Pf6N2E2_783at 896.764 kb on - strand, within Pf6N2E2_783at 896.825 kb on - strand, within Pf6N2E2_783at 897.005 kb on + strand, within Pf6N2E2_783at 897.005 kb on + strand, within Pf6N2E2_783at 897.005 kb on + strand, within Pf6N2E2_783at 897.006 kb on - strand, within Pf6N2E2_783at 897.006 kb on - strand, within Pf6N2E2_783at 897.006 kb on - strand, within Pf6N2E2_783at 897.027 kb on + strand, within Pf6N2E2_783at 897.027 kb on + strand, within Pf6N2E2_783at 897.028 kb on - strand, within Pf6N2E2_783at 897.028 kb on - strand, within Pf6N2E2_783at 897.028 kb on - strand, within Pf6N2E2_783at 897.034 kb on + strand, within Pf6N2E2_783at 897.034 kb on + strand, within Pf6N2E2_783at 897.034 kb on + strand, within Pf6N2E2_783at 897.035 kb on - strand, within Pf6N2E2_783at 897.035 kb on - strand, within Pf6N2E2_783at 897.086 kb on + strand, within Pf6N2E2_783at 897.174 kb on + strandat 897.174 kb on + strand, within Pf6N2E2_783at 897.175 kb on - strand, within Pf6N2E2_783at 897.175 kb on - strand, within Pf6N2E2_783at 897.175 kb on - strand, within Pf6N2E2_783at 897.285 kb on + strand, within Pf6N2E2_783at 897.361 kb on + strand, within Pf6N2E2_783at 897.383 kb on + strand, within Pf6N2E2_783at 897.383 kb on + strand, within Pf6N2E2_783at 897.383 kb on + strand, within Pf6N2E2_783at 897.383 kb on + strand, within Pf6N2E2_783at 897.383 kb on + strand, within Pf6N2E2_783at 897.383 kb on + strand, within Pf6N2E2_783at 897.383 kb on + strand, within Pf6N2E2_783at 897.383 kb on + strand, within Pf6N2E2_783at 897.383 kb on + strand, within Pf6N2E2_783at 897.384 kb on - strand, within Pf6N2E2_783at 897.558 kb on + strandat 897.558 kb on + strandat 897.558 kb on + strandat 897.559 kb on - strandat 897.559 kb on - strandat 897.697 kb on + strandat 897.697 kb on + strandat 897.731 kb on + strandat 897.731 kb on + strandat 897.731 kb on + strandat 897.731 kb on + strandat 897.732 kb on - strandat 898.062 kb on - strand, within Pf6N2E2_784at 898.062 kb on - strand, within Pf6N2E2_784at 898.062 kb on - strand, within Pf6N2E2_784at 898.062 kb on - strand, within Pf6N2E2_784at 898.062 kb on - strand, within Pf6N2E2_784at 898.161 kb on + strand, within Pf6N2E2_784at 898.217 kb on + strand, within Pf6N2E2_784at 898.217 kb on + strand, within Pf6N2E2_784at 898.218 kb on - strand, within Pf6N2E2_784at 898.218 kb on - strand, within Pf6N2E2_784at 898.272 kb on + strand, within Pf6N2E2_784at 898.297 kb on + strand, within Pf6N2E2_784at 898.343 kb on + strand, within Pf6N2E2_784at 898.344 kb on - strand, within Pf6N2E2_784at 898.391 kb on + strand, within Pf6N2E2_784at 898.391 kb on + strand, within Pf6N2E2_784at 898.392 kb on - strand, within Pf6N2E2_784at 898.392 kb on - strand, within Pf6N2E2_784at 898.438 kb on + strand, within Pf6N2E2_784at 898.461 kb on + strand, within Pf6N2E2_784at 898.462 kb on - strand, within Pf6N2E2_784at 898.462 kb on - strand, within Pf6N2E2_784at 898.468 kb on + strand, within Pf6N2E2_784at 898.470 kb on + strand, within Pf6N2E2_784at 898.470 kb on + strand, within Pf6N2E2_784at 898.471 kb on - strand, within Pf6N2E2_784at 898.758 kb on + strand, within Pf6N2E2_784at 898.897 kb on - strand, within Pf6N2E2_784at 898.897 kb on - strand, within Pf6N2E2_784at 898.897 kb on - strand, within Pf6N2E2_784at 898.897 kb on - strand, within Pf6N2E2_784at 898.897 kb on - strand, within Pf6N2E2_784at 898.897 kb on - strand, within Pf6N2E2_784at 898.898 kb on + strand, within Pf6N2E2_784at 898.898 kb on + strand, within Pf6N2E2_784at 898.898 kb on + strand, within Pf6N2E2_784at 898.898 kb on + strand, within Pf6N2E2_784at 898.898 kb on + strand, within Pf6N2E2_784at 898.898 kb on + strand, within Pf6N2E2_784at 898.898 kb on + strand, within Pf6N2E2_784at 898.898 kb on + strand, within Pf6N2E2_784at 898.898 kb on + strand, within Pf6N2E2_784at 898.899 kb on - strand, within Pf6N2E2_784at 898.899 kb on - strand, within Pf6N2E2_784at 898.933 kb on + strand, within Pf6N2E2_784at 898.933 kb on + strand, within Pf6N2E2_784at 898.934 kb on - strand, within Pf6N2E2_784at 898.934 kb on - strand, within Pf6N2E2_784at 898.934 kb on - strand, within Pf6N2E2_784at 898.934 kb on - strand, within Pf6N2E2_784at 898.977 kb on + strand, within Pf6N2E2_784at 898.977 kb on + strand, within Pf6N2E2_784at 898.977 kb on + strand, within Pf6N2E2_784at 898.977 kb on + strand, within Pf6N2E2_784at 898.978 kb on - strand, within Pf6N2E2_784at 898.978 kb on - strand, within Pf6N2E2_784at 898.978 kb on - strand, within Pf6N2E2_784at 898.978 kb on - strand, within Pf6N2E2_784at 899.041 kb on - strand, within Pf6N2E2_784at 899.084 kb on + strand, within Pf6N2E2_784at 899.085 kb on - strand, within Pf6N2E2_784at 899.136 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.155 kb on - strand, within Pf6N2E2_784at 899.155 kb on - strand, within Pf6N2E2_784at 899.155 kb on - strand, within Pf6N2E2_784at 899.155 kb on - strand, within Pf6N2E2_784at 899.164 kb on - strandat 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.380 kb on - strand, within Pf6N2E2_784at 899.383 kb on + strand, within Pf6N2E2_784at 899.383 kb on + strand, within Pf6N2E2_784at 899.383 kb on + strand, within Pf6N2E2_784at 899.383 kb on + strand, within Pf6N2E2_784at 899.383 kb on + strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.385 kb on + strand, within Pf6N2E2_784at 899.385 kb on + strand, within Pf6N2E2_784at 899.481 kb on + strand, within Pf6N2E2_784at 899.544 kb on + strand, within Pf6N2E2_784at 899.544 kb on + strand, within Pf6N2E2_784at 899.544 kb on + strand, within Pf6N2E2_784at 899.544 kb on - strand, within Pf6N2E2_784at 899.671 kb on + strand, within Pf6N2E2_784at 899.688 kb on - strand, within Pf6N2E2_784at 899.750 kb on + strand, within Pf6N2E2_784at 899.750 kb on + strand, within Pf6N2E2_784at 899.750 kb on + strand, within Pf6N2E2_784at 899.751 kb on - strand, within Pf6N2E2_784at 899.751 kb on - strand, within Pf6N2E2_784at 899.751 kb on - strand, within Pf6N2E2_784at 900.098 kb on + strandat 900.278 kb on + strand, within Pf6N2E2_785at 900.279 kb on - strand, within Pf6N2E2_785at 900.352 kb on + strand, within Pf6N2E2_785at 900.352 kb on + strand, within Pf6N2E2_785at 900.352 kb on + strand, within Pf6N2E2_785at 900.479 kb on + strand, within Pf6N2E2_785at 900.535 kb on + strand, within Pf6N2E2_785at 900.535 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.700 kb on - strand, within Pf6N2E2_785at 900.700 kb on - strand, within Pf6N2E2_785at 900.701 kb on + strand, within Pf6N2E2_785at 900.892 kb on + strandat 900.958 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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896,614 + Pf6N2E2_783 0.17 -0.7
896,614 + Pf6N2E2_783 0.17 +1.2
896,615 - Pf6N2E2_783 0.17 +0.6
896,763 + Pf6N2E2_783 0.30 -0.4
896,763 + Pf6N2E2_783 0.30 +0.0
896,763 + Pf6N2E2_783 0.30 +0.7
896,763 + Pf6N2E2_783 0.30 +1.8
896,764 - Pf6N2E2_783 0.30 +0.4
896,825 - Pf6N2E2_783 0.36 -1.4
897,005 + Pf6N2E2_783 0.52 +1.3
897,005 + Pf6N2E2_783 0.52 -1.8
897,005 + Pf6N2E2_783 0.52 -0.5
897,006 - Pf6N2E2_783 0.52 +1.0
897,006 - Pf6N2E2_783 0.52 -2.0
897,006 - Pf6N2E2_783 0.52 +0.6
897,027 + Pf6N2E2_783 0.54 -0.3
897,027 + Pf6N2E2_783 0.54 -2.1
897,028 - Pf6N2E2_783 0.54 -0.4
897,028 - Pf6N2E2_783 0.54 -0.6
897,028 - Pf6N2E2_783 0.54 +1.8
897,034 + Pf6N2E2_783 0.55 -0.1
897,034 + Pf6N2E2_783 0.55 +0.4
897,034 + Pf6N2E2_783 0.55 -2.2
897,035 - Pf6N2E2_783 0.55 -0.9
897,035 - Pf6N2E2_783 0.55 -0.4
897,086 + Pf6N2E2_783 0.60 -1.2
897,174 + -0.1
897,174 + Pf6N2E2_783 0.68 +1.4
897,175 - Pf6N2E2_783 0.68 +0.3
897,175 - Pf6N2E2_783 0.68 -0.7
897,175 - Pf6N2E2_783 0.68 -0.7
897,285 + Pf6N2E2_783 0.78 +0.4
897,361 + Pf6N2E2_783 0.85 +0.9
897,383 + Pf6N2E2_783 0.87 +0.1
897,383 + Pf6N2E2_783 0.87 -1.2
897,383 + Pf6N2E2_783 0.87 -0.3
897,383 + Pf6N2E2_783 0.87 +0.1
897,383 + Pf6N2E2_783 0.87 +1.4
897,383 + Pf6N2E2_783 0.87 -1.1
897,383 + Pf6N2E2_783 0.87 +0.7
897,383 + Pf6N2E2_783 0.87 +1.2
897,383 + Pf6N2E2_783 0.87 -0.4
897,384 - Pf6N2E2_783 0.87 -0.5
897,558 + -0.0
897,558 + +0.0
897,558 + -0.4
897,559 - -0.3
897,559 - -0.9
897,697 + +0.6
897,697 + +1.0
897,731 + -0.5
897,731 + -0.1
897,731 + -0.2
897,731 + +0.1
897,732 - -0.5
898,062 - Pf6N2E2_784 0.19 +0.8
898,062 - Pf6N2E2_784 0.19 -1.5
898,062 - Pf6N2E2_784 0.19 -0.3
898,062 - Pf6N2E2_784 0.19 -1.7
898,062 - Pf6N2E2_784 0.19 -0.5
898,161 + Pf6N2E2_784 0.23 +0.7
898,217 + Pf6N2E2_784 0.25 -0.8
898,217 + Pf6N2E2_784 0.25 -0.0
898,218 - Pf6N2E2_784 0.25 +0.3
898,218 - Pf6N2E2_784 0.25 +0.3
898,272 + Pf6N2E2_784 0.27 +0.1
898,297 + Pf6N2E2_784 0.28 +1.0
898,343 + Pf6N2E2_784 0.30 -0.5
898,344 - Pf6N2E2_784 0.30 -0.0
898,391 + Pf6N2E2_784 0.32 -0.3
898,391 + Pf6N2E2_784 0.32 +0.6
898,392 - Pf6N2E2_784 0.32 -0.2
898,392 - Pf6N2E2_784 0.32 -0.1
898,438 + Pf6N2E2_784 0.34 +0.2
898,461 + Pf6N2E2_784 0.35 +0.4
898,462 - Pf6N2E2_784 0.35 -1.3
898,462 - Pf6N2E2_784 0.35 -2.8
898,468 + Pf6N2E2_784 0.35 -0.5
898,470 + Pf6N2E2_784 0.35 -0.7
898,470 + Pf6N2E2_784 0.35 +0.7
898,471 - Pf6N2E2_784 0.35 -0.9
898,758 + Pf6N2E2_784 0.47 +0.2
898,897 - Pf6N2E2_784 0.52 +0.2
898,897 - Pf6N2E2_784 0.52 +0.2
898,897 - Pf6N2E2_784 0.52 +0.7
898,897 - Pf6N2E2_784 0.52 +0.0
898,897 - Pf6N2E2_784 0.52 -0.4
898,897 - Pf6N2E2_784 0.52 -1.9
898,898 + Pf6N2E2_784 0.52 -1.6
898,898 + Pf6N2E2_784 0.52 +0.0
898,898 + Pf6N2E2_784 0.52 +0.2
898,898 + Pf6N2E2_784 0.52 -0.9
898,898 + Pf6N2E2_784 0.52 +1.0
898,898 + Pf6N2E2_784 0.52 +0.5
898,898 + Pf6N2E2_784 0.52 +1.1
898,898 + Pf6N2E2_784 0.52 -0.3
898,898 + Pf6N2E2_784 0.52 -0.0
898,899 - Pf6N2E2_784 0.52 -0.0
898,899 - Pf6N2E2_784 0.52 +1.0
898,933 + Pf6N2E2_784 0.54 -0.5
898,933 + Pf6N2E2_784 0.54 +2.3
898,934 - Pf6N2E2_784 0.54 -1.3
898,934 - Pf6N2E2_784 0.54 +1.0
898,934 - Pf6N2E2_784 0.54 -0.0
898,934 - Pf6N2E2_784 0.54 +0.8
898,977 + Pf6N2E2_784 0.56 -0.5
898,977 + Pf6N2E2_784 0.56 -1.4
898,977 + Pf6N2E2_784 0.56 -0.0
898,977 + Pf6N2E2_784 0.56 -0.7
898,978 - Pf6N2E2_784 0.56 -2.9
898,978 - Pf6N2E2_784 0.56 -1.7
898,978 - Pf6N2E2_784 0.56 +2.0
898,978 - Pf6N2E2_784 0.56 -0.5
899,041 - Pf6N2E2_784 0.58 -0.1
899,084 + Pf6N2E2_784 0.60 +0.5
899,085 - Pf6N2E2_784 0.60 -1.2
899,136 + Pf6N2E2_784 0.62 -0.2
899,154 + Pf6N2E2_784 0.63 +1.0
899,154 + Pf6N2E2_784 0.63 -0.5
899,154 + Pf6N2E2_784 0.63 -0.1
899,154 + Pf6N2E2_784 0.63 -2.7
899,154 + Pf6N2E2_784 0.63 +0.9
899,154 + Pf6N2E2_784 0.63 +0.3
899,154 + Pf6N2E2_784 0.63 -0.3
899,155 - Pf6N2E2_784 0.63 +0.3
899,155 - Pf6N2E2_784 0.63 +1.4
899,155 - Pf6N2E2_784 0.63 -0.6
899,155 - Pf6N2E2_784 0.63 -0.9
899,164 - +3.0
899,379 + Pf6N2E2_784 0.72 -0.7
899,379 + Pf6N2E2_784 0.72 -2.1
899,379 + Pf6N2E2_784 0.72 -0.8
899,379 + Pf6N2E2_784 0.72 +0.3
899,379 + Pf6N2E2_784 0.72 +0.4
899,379 + Pf6N2E2_784 0.72 -1.2
899,379 + Pf6N2E2_784 0.72 -1.2
899,380 - Pf6N2E2_784 0.72 +1.2
899,383 + Pf6N2E2_784 0.72 -0.1
899,383 + Pf6N2E2_784 0.72 -0.1
899,383 + Pf6N2E2_784 0.72 +1.2
899,383 + Pf6N2E2_784 0.72 -2.0
899,383 + Pf6N2E2_784 0.72 -0.9
899,384 - Pf6N2E2_784 0.72 -2.0
899,384 - Pf6N2E2_784 0.72 -1.1
899,384 - Pf6N2E2_784 0.72 -0.2
899,384 - Pf6N2E2_784 0.72 -0.8
899,384 - Pf6N2E2_784 0.72 -1.0
899,384 - Pf6N2E2_784 0.72 +0.0
899,384 - Pf6N2E2_784 0.72 -0.7
899,384 - Pf6N2E2_784 0.72 +0.0
899,385 + Pf6N2E2_784 0.72 +0.0
899,385 + Pf6N2E2_784 0.72 +0.5
899,481 + Pf6N2E2_784 0.76 +0.1
899,544 + Pf6N2E2_784 0.78 +0.0
899,544 + Pf6N2E2_784 0.78 -0.1
899,544 + Pf6N2E2_784 0.78 +0.7
899,544 - Pf6N2E2_784 0.78 -1.1
899,671 + Pf6N2E2_784 0.83 -0.6
899,688 - Pf6N2E2_784 0.84 +0.3
899,750 + Pf6N2E2_784 0.87 +0.8
899,750 + Pf6N2E2_784 0.87 -2.6
899,750 + Pf6N2E2_784 0.87 +0.2
899,751 - Pf6N2E2_784 0.87 +0.7
899,751 - Pf6N2E2_784 0.87 -0.1
899,751 - Pf6N2E2_784 0.87 +0.1
900,098 + -1.9
900,278 + Pf6N2E2_785 0.20 +0.9
900,279 - Pf6N2E2_785 0.20 +1.7
900,352 + Pf6N2E2_785 0.30 -0.1
900,352 + Pf6N2E2_785 0.30 -0.0
900,352 + Pf6N2E2_785 0.30 +0.6
900,479 + Pf6N2E2_785 0.47 +1.3
900,535 + Pf6N2E2_785 0.54 -1.0
900,535 + Pf6N2E2_785 0.54 -1.2
900,699 + Pf6N2E2_785 0.76 -1.2
900,699 + Pf6N2E2_785 0.76 +0.6
900,699 + Pf6N2E2_785 0.76 +0.7
900,699 + Pf6N2E2_785 0.76 +0.5
900,699 + Pf6N2E2_785 0.76 -1.5
900,700 - Pf6N2E2_785 0.76 +0.0
900,700 - Pf6N2E2_785 0.76 -2.4
900,701 + Pf6N2E2_785 0.76 -0.6
900,892 + -1.0
900,958 + -1.5

Or see this region's nucleotide sequence