Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5942 and Pf6N2E2_5943 overlap by 21 nucleotides Pf6N2E2_5943 and Pf6N2E2_5944 are separated by 312 nucleotides Pf6N2E2_5944 and Pf6N2E2_5945 overlap by 11 nucleotides Pf6N2E2_5945 and Pf6N2E2_5946 are separated by 20 nucleotides
Pf6N2E2_5942 - Ribonuclease E (EC 3.1.26.12), at 6,761,732 to 6,764,929
_5942
Pf6N2E2_5943 - hypothetical protein, at 6,764,909 to 6,765,313
_5943
Pf6N2E2_5944 - Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70), at 6,765,626 to 6,766,594
_5944
Pf6N2E2_5945 - Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C, at 6,766,584 to 6,767,246
_5945
Pf6N2E2_5946 - Periplasmic serine proteases (ClpP class), at 6,767,267 to 6,768,256
_5946
Position (kb)
6765
6766
6767 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6765.269 kb on - strand, within Pf6N2E2_5943 at 6765.269 kb on - strand, within Pf6N2E2_5943 at 6765.269 kb on - strand, within Pf6N2E2_5943 at 6765.307 kb on - strand at 6765.307 kb on - strand at 6765.307 kb on - strand at 6765.307 kb on - strand at 6765.421 kb on - strand at 6765.598 kb on - strand at 6765.625 kb on + strand at 6765.646 kb on + strand at 6765.646 kb on + strand at 6765.646 kb on + strand at 6765.646 kb on + strand at 6765.647 kb on - strand at 6765.716 kb on - strand at 6765.818 kb on + strand, within Pf6N2E2_5944 at 6765.928 kb on + strand, within Pf6N2E2_5944 at 6765.928 kb on + strand, within Pf6N2E2_5944 at 6765.928 kb on + strand, within Pf6N2E2_5944 at 6765.932 kb on + strand, within Pf6N2E2_5944 at 6765.932 kb on + strand, within Pf6N2E2_5944 at 6765.932 kb on + strand, within Pf6N2E2_5944 at 6765.973 kb on + strand, within Pf6N2E2_5944 at 6766.170 kb on - strand, within Pf6N2E2_5944 at 6766.387 kb on + strand, within Pf6N2E2_5944 at 6766.387 kb on + strand, within Pf6N2E2_5944 at 6766.388 kb on - strand, within Pf6N2E2_5944 at 6766.428 kb on - strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.494 kb on - strand, within Pf6N2E2_5944 at 6766.596 kb on + strand at 6766.689 kb on + strand, within Pf6N2E2_5945 at 6766.708 kb on + strand, within Pf6N2E2_5945 at 6766.748 kb on + strand, within Pf6N2E2_5945 at 6766.818 kb on + strand, within Pf6N2E2_5945 at 6766.819 kb on - strand, within Pf6N2E2_5945 at 6766.896 kb on + strand, within Pf6N2E2_5945 at 6766.971 kb on + strand, within Pf6N2E2_5945 at 6766.971 kb on + strand, within Pf6N2E2_5945 at 6766.972 kb on - strand, within Pf6N2E2_5945 at 6767.145 kb on + strand, within Pf6N2E2_5945 at 6767.393 kb on - strand, within Pf6N2E2_5946 at 6767.393 kb on - strand, within Pf6N2E2_5946 at 6767.416 kb on + strand, within Pf6N2E2_5946 at 6767.416 kb on + strand, within Pf6N2E2_5946 at 6767.416 kb on + strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.420 kb on + strand, within Pf6N2E2_5946 at 6767.421 kb on - strand, within Pf6N2E2_5946 at 6767.421 kb on - strand, within Pf6N2E2_5946 at 6767.493 kb on - strand, within Pf6N2E2_5946 at 6767.493 kb on - strand, within Pf6N2E2_5946
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 6,765,269 - Pf6N2E2_5943 0.89 +0.8 6,765,269 - Pf6N2E2_5943 0.89 +0.2 6,765,269 - Pf6N2E2_5943 0.89 -2.0 6,765,307 - +1.9 6,765,307 - -1.6 6,765,307 - -1.0 6,765,307 - +1.2 6,765,421 - +0.2 6,765,598 - +2.9 6,765,625 + -1.1 6,765,646 + -0.8 6,765,646 + -1.4 6,765,646 + -3.0 6,765,646 + -1.3 6,765,647 - +0.0 6,765,716 - -0.4 6,765,818 + Pf6N2E2_5944 0.20 -1.4 6,765,928 + Pf6N2E2_5944 0.31 +0.7 6,765,928 + Pf6N2E2_5944 0.31 -0.8 6,765,928 + Pf6N2E2_5944 0.31 -1.2 6,765,932 + Pf6N2E2_5944 0.32 +0.6 6,765,932 + Pf6N2E2_5944 0.32 -2.9 6,765,932 + Pf6N2E2_5944 0.32 -1.1 6,765,973 + Pf6N2E2_5944 0.36 -1.5 6,766,170 - Pf6N2E2_5944 0.56 +0.2 6,766,387 + Pf6N2E2_5944 0.79 -0.9 6,766,387 + Pf6N2E2_5944 0.79 -1.5 6,766,388 - Pf6N2E2_5944 0.79 -1.0 6,766,428 - Pf6N2E2_5944 0.83 -0.8 6,766,493 + Pf6N2E2_5944 0.89 -0.9 6,766,493 + Pf6N2E2_5944 0.89 -1.1 6,766,493 + Pf6N2E2_5944 0.89 -2.1 6,766,493 + Pf6N2E2_5944 0.89 -1.5 6,766,493 + Pf6N2E2_5944 0.89 -1.3 6,766,493 + Pf6N2E2_5944 0.89 -0.9 6,766,493 + Pf6N2E2_5944 0.89 -0.6 6,766,493 + Pf6N2E2_5944 0.89 -1.1 6,766,493 + Pf6N2E2_5944 0.89 -1.5 6,766,493 + Pf6N2E2_5944 0.89 -1.5 6,766,493 + Pf6N2E2_5944 0.89 -1.8 6,766,494 - Pf6N2E2_5944 0.90 -2.3 6,766,596 + -1.7 6,766,689 + Pf6N2E2_5945 0.16 -1.8 6,766,708 + Pf6N2E2_5945 0.19 -0.1 6,766,748 + Pf6N2E2_5945 0.25 -0.4 6,766,818 + Pf6N2E2_5945 0.35 -0.0 6,766,819 - Pf6N2E2_5945 0.35 -1.2 6,766,896 + Pf6N2E2_5945 0.47 -0.6 6,766,971 + Pf6N2E2_5945 0.58 -0.7 6,766,971 + Pf6N2E2_5945 0.58 -1.8 6,766,972 - Pf6N2E2_5945 0.59 -0.4 6,767,145 + Pf6N2E2_5945 0.85 -1.8 6,767,393 - Pf6N2E2_5946 0.13 -0.0 6,767,393 - Pf6N2E2_5946 0.13 +1.2 6,767,416 + Pf6N2E2_5946 0.15 -0.5 6,767,416 + Pf6N2E2_5946 0.15 +1.1 6,767,416 + Pf6N2E2_5946 0.15 -0.9 6,767,417 - Pf6N2E2_5946 0.15 +0.7 6,767,417 - Pf6N2E2_5946 0.15 +0.5 6,767,417 - Pf6N2E2_5946 0.15 +0.7 6,767,417 - Pf6N2E2_5946 0.15 -0.4 6,767,417 - Pf6N2E2_5946 0.15 -0.5 6,767,417 - Pf6N2E2_5946 0.15 -0.2 6,767,417 - Pf6N2E2_5946 0.15 +0.2 6,767,417 - Pf6N2E2_5946 0.15 -1.3 6,767,417 - Pf6N2E2_5946 0.15 +0.3 6,767,417 - Pf6N2E2_5946 0.15 +0.7 6,767,420 + Pf6N2E2_5946 0.15 +0.4 6,767,421 - Pf6N2E2_5946 0.16 +1.5 6,767,421 - Pf6N2E2_5946 0.16 +0.9 6,767,493 - Pf6N2E2_5946 0.23 -0.0 6,767,493 - Pf6N2E2_5946 0.23 +1.8
Or see this region's nucleotide sequence