Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5877 and Pf6N2E2_5878 are separated by 52 nucleotides Pf6N2E2_5878 and Pf6N2E2_5879 are separated by 296 nucleotides Pf6N2E2_5879 and Pf6N2E2_5880 overlap by 4 nucleotides Pf6N2E2_5880 and Pf6N2E2_5881 are separated by 129 nucleotides
Pf6N2E2_5877 - Phosphohistidine phosphatase SixA, at 6,698,039 to 6,698,491
_5877
Pf6N2E2_5878 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 6,698,544 to 6,698,987
_5878
Pf6N2E2_5879 - FIG00955363: hypothetical protein, at 6,699,284 to 6,700,078
_5879
Pf6N2E2_5880 - hydrolase, alpha/beta fold family, at 6,700,075 to 6,700,926
_5880
Pf6N2E2_5881 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 6,701,056 to 6,701,874
_5881
Position (kb)
6699
6700
6701 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6698.600 kb on + strand, within Pf6N2E2_5878 at 6698.600 kb on + strand, within Pf6N2E2_5878 at 6698.601 kb on - strand, within Pf6N2E2_5878 at 6698.757 kb on + strand, within Pf6N2E2_5878 at 6698.757 kb on + strand, within Pf6N2E2_5878 at 6698.758 kb on - strand, within Pf6N2E2_5878 at 6698.758 kb on - strand, within Pf6N2E2_5878 at 6698.758 kb on - strand, within Pf6N2E2_5878 at 6698.758 kb on - strand, within Pf6N2E2_5878 at 6698.758 kb on - strand, within Pf6N2E2_5878 at 6698.784 kb on + strand, within Pf6N2E2_5878 at 6698.785 kb on - strand, within Pf6N2E2_5878 at 6698.785 kb on - strand, within Pf6N2E2_5878 at 6698.785 kb on - strand, within Pf6N2E2_5878 at 6698.785 kb on - strand, within Pf6N2E2_5878 at 6698.785 kb on - strand, within Pf6N2E2_5878 at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.997 kb on - strand at 6698.997 kb on - strand at 6698.997 kb on - strand at 6698.997 kb on - strand at 6698.997 kb on - strand at 6699.069 kb on - strand at 6699.069 kb on - strand at 6699.173 kb on + strand at 6699.173 kb on + strand at 6699.217 kb on + strand at 6699.217 kb on + strand at 6699.335 kb on + strand at 6699.369 kb on - strand, within Pf6N2E2_5879 at 6699.551 kb on + strand, within Pf6N2E2_5879 at 6699.765 kb on - strand, within Pf6N2E2_5879 at 6699.840 kb on - strand, within Pf6N2E2_5879 at 6699.958 kb on + strand, within Pf6N2E2_5879 at 6699.958 kb on + strand, within Pf6N2E2_5879 at 6699.958 kb on + strand, within Pf6N2E2_5879 at 6699.958 kb on + strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.997 kb on + strand, within Pf6N2E2_5879 at 6699.997 kb on + strand, within Pf6N2E2_5879 at 6699.998 kb on - strand, within Pf6N2E2_5879 at 6699.998 kb on - strand, within Pf6N2E2_5879 at 6700.160 kb on + strand at 6700.310 kb on - strand, within Pf6N2E2_5880 at 6700.310 kb on - strand, within Pf6N2E2_5880 at 6700.549 kb on + strand, within Pf6N2E2_5880 at 6700.581 kb on + strand, within Pf6N2E2_5880 at 6700.623 kb on - strand, within Pf6N2E2_5880 at 6700.660 kb on + strand, within Pf6N2E2_5880 at 6700.661 kb on - strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.856 kb on + strand at 6700.856 kb on + strand at 6700.856 kb on + strand at 6700.856 kb on + strand at 6700.856 kb on + strand at 6700.857 kb on - strand at 6700.857 kb on - strand at 6700.857 kb on - strand at 6700.857 kb on - strand at 6700.890 kb on - strand at 6700.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 6,698,600 + Pf6N2E2_5878 0.13 -0.1 6,698,600 + Pf6N2E2_5878 0.13 +0.3 6,698,601 - Pf6N2E2_5878 0.13 +0.1 6,698,757 + Pf6N2E2_5878 0.48 -1.9 6,698,757 + Pf6N2E2_5878 0.48 +1.8 6,698,758 - Pf6N2E2_5878 0.48 -1.7 6,698,758 - Pf6N2E2_5878 0.48 -0.3 6,698,758 - Pf6N2E2_5878 0.48 +1.1 6,698,758 - Pf6N2E2_5878 0.48 +0.5 6,698,758 - Pf6N2E2_5878 0.48 -0.6 6,698,784 + Pf6N2E2_5878 0.54 -0.2 6,698,785 - Pf6N2E2_5878 0.54 -0.9 6,698,785 - Pf6N2E2_5878 0.54 -0.9 6,698,785 - Pf6N2E2_5878 0.54 -0.2 6,698,785 - Pf6N2E2_5878 0.54 -2.5 6,698,785 - Pf6N2E2_5878 0.54 +0.6 6,698,996 + -0.5 6,698,996 + +0.5 6,698,996 + +1.1 6,698,996 + +0.1 6,698,996 + -1.7 6,698,996 + +0.6 6,698,996 + -1.4 6,698,996 + -0.7 6,698,996 + -0.6 6,698,996 + +0.5 6,698,996 + +0.3 6,698,996 + -0.4 6,698,997 - +0.4 6,698,997 - -1.7 6,698,997 - +0.7 6,698,997 - +0.1 6,698,997 - -2.3 6,699,069 - -0.2 6,699,069 - -0.6 6,699,173 + +0.2 6,699,173 + -0.7 6,699,217 + +0.3 6,699,217 + +1.4 6,699,335 + +1.2 6,699,369 - Pf6N2E2_5879 0.11 -0.5 6,699,551 + Pf6N2E2_5879 0.34 +0.0 6,699,765 - Pf6N2E2_5879 0.61 +1.5 6,699,840 - Pf6N2E2_5879 0.70 +0.1 6,699,958 + Pf6N2E2_5879 0.85 -1.5 6,699,958 + Pf6N2E2_5879 0.85 -1.8 6,699,958 + Pf6N2E2_5879 0.85 -1.2 6,699,958 + Pf6N2E2_5879 0.85 +1.1 6,699,959 - Pf6N2E2_5879 0.85 +0.3 6,699,959 - Pf6N2E2_5879 0.85 +0.2 6,699,959 - Pf6N2E2_5879 0.85 -1.7 6,699,959 - Pf6N2E2_5879 0.85 -2.7 6,699,959 - Pf6N2E2_5879 0.85 +0.4 6,699,959 - Pf6N2E2_5879 0.85 -1.3 6,699,959 - Pf6N2E2_5879 0.85 +0.7 6,699,959 - Pf6N2E2_5879 0.85 +0.1 6,699,959 - Pf6N2E2_5879 0.85 -1.0 6,699,959 - Pf6N2E2_5879 0.85 -1.8 6,699,959 - Pf6N2E2_5879 0.85 -0.4 6,699,959 - Pf6N2E2_5879 0.85 -1.2 6,699,959 - Pf6N2E2_5879 0.85 -0.4 6,699,959 - Pf6N2E2_5879 0.85 -0.5 6,699,959 - Pf6N2E2_5879 0.85 +0.2 6,699,997 + Pf6N2E2_5879 0.90 +1.0 6,699,997 + Pf6N2E2_5879 0.90 +0.3 6,699,998 - Pf6N2E2_5879 0.90 -1.3 6,699,998 - Pf6N2E2_5879 0.90 -0.9 6,700,160 + -1.3 6,700,310 - Pf6N2E2_5880 0.28 -0.7 6,700,310 - Pf6N2E2_5880 0.28 -0.4 6,700,549 + Pf6N2E2_5880 0.56 +0.5 6,700,581 + Pf6N2E2_5880 0.59 -0.5 6,700,623 - Pf6N2E2_5880 0.64 -0.3 6,700,660 + Pf6N2E2_5880 0.69 +0.9 6,700,661 - Pf6N2E2_5880 0.69 +0.1 6,700,662 + Pf6N2E2_5880 0.69 +0.4 6,700,662 + Pf6N2E2_5880 0.69 +0.1 6,700,662 + Pf6N2E2_5880 0.69 +1.6 6,700,662 + Pf6N2E2_5880 0.69 -1.9 6,700,662 + Pf6N2E2_5880 0.69 -3.5 6,700,662 + Pf6N2E2_5880 0.69 -0.4 6,700,662 + Pf6N2E2_5880 0.69 +0.9 6,700,662 + Pf6N2E2_5880 0.69 -1.0 6,700,662 + Pf6N2E2_5880 0.69 -0.1 6,700,662 + Pf6N2E2_5880 0.69 -0.8 6,700,662 + Pf6N2E2_5880 0.69 -0.8 6,700,663 - Pf6N2E2_5880 0.69 +3.5 6,700,663 - Pf6N2E2_5880 0.69 -1.4 6,700,663 - Pf6N2E2_5880 0.69 -0.9 6,700,663 - Pf6N2E2_5880 0.69 +0.2 6,700,663 - Pf6N2E2_5880 0.69 +0.2 6,700,663 - Pf6N2E2_5880 0.69 +2.2 6,700,663 - Pf6N2E2_5880 0.69 -0.5 6,700,663 - Pf6N2E2_5880 0.69 -0.8 6,700,663 - Pf6N2E2_5880 0.69 +0.6 6,700,663 - Pf6N2E2_5880 0.69 -1.8 6,700,663 - Pf6N2E2_5880 0.69 -0.3 6,700,663 - Pf6N2E2_5880 0.69 -1.8 6,700,663 - Pf6N2E2_5880 0.69 -0.5 6,700,663 - Pf6N2E2_5880 0.69 +1.5 6,700,663 - Pf6N2E2_5880 0.69 +1.3 6,700,663 - Pf6N2E2_5880 0.69 +0.2 6,700,663 - Pf6N2E2_5880 0.69 -1.0 6,700,663 - Pf6N2E2_5880 0.69 -0.8 6,700,856 + -1.2 6,700,856 + +1.2 6,700,856 + -0.6 6,700,856 + +2.4 6,700,856 + +0.1 6,700,857 - +0.0 6,700,857 - +0.2 6,700,857 - +0.4 6,700,857 - -1.0 6,700,890 - +1.7 6,700,993 - +1.3
Or see this region's nucleotide sequence