Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5870 and Pf6N2E2_5871 are separated by 11 nucleotides Pf6N2E2_5871 and Pf6N2E2_5872 are separated by 248 nucleotides Pf6N2E2_5872 and Pf6N2E2_5873 overlap by 4 nucleotides Pf6N2E2_5873 and Pf6N2E2_5874 are separated by 0 nucleotides
Pf6N2E2_5870 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASI (EC 2.3.1.41), at 6,689,489 to 6,690,709
_5870
Pf6N2E2_5871 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, FabA form (EC 4.2.1.59), at 6,690,721 to 6,691,236
_5871
Pf6N2E2_5872 - Sensor histidine kinase/response regulator, at 6,691,485 to 6,693,386
_5872
Pf6N2E2_5873 - FIG00953467: hypothetical protein, at 6,693,383 to 6,694,315
_5873
Pf6N2E2_5874 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 6,694,316 to 6,696,400
_5874
Position (kb)
6691
6692
6693
6694 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 6691.374 kb on - strand at 6691.374 kb on - strand at 6691.374 kb on - strand at 6691.430 kb on - strand at 6691.486 kb on + strand at 6691.486 kb on + strand at 6691.486 kb on + strand at 6691.487 kb on - strand at 6691.487 kb on - strand at 6691.487 kb on - strand at 6691.547 kb on - strand at 6691.547 kb on - strand at 6691.734 kb on + strand, within Pf6N2E2_5872 at 6691.734 kb on + strand, within Pf6N2E2_5872 at 6691.734 kb on + strand, within Pf6N2E2_5872 at 6691.734 kb on + strand, within Pf6N2E2_5872 at 6691.735 kb on - strand, within Pf6N2E2_5872 at 6691.836 kb on + strand, within Pf6N2E2_5872 at 6691.836 kb on + strand, within Pf6N2E2_5872 at 6691.836 kb on + strand, within Pf6N2E2_5872 at 6691.837 kb on - strand, within Pf6N2E2_5872 at 6691.837 kb on - strand, within Pf6N2E2_5872 at 6691.837 kb on - strand, within Pf6N2E2_5872 at 6691.837 kb on - strand, within Pf6N2E2_5872 at 6691.837 kb on - strand, within Pf6N2E2_5872 at 6691.837 kb on - strand, within Pf6N2E2_5872 at 6691.837 kb on - strand, within Pf6N2E2_5872 at 6691.837 kb on - strand, within Pf6N2E2_5872 at 6691.837 kb on - strand, within Pf6N2E2_5872 at 6692.482 kb on + strand, within Pf6N2E2_5872 at 6692.482 kb on + strand, within Pf6N2E2_5872 at 6692.482 kb on + strand, within Pf6N2E2_5872 at 6692.482 kb on + strand, within Pf6N2E2_5872 at 6692.483 kb on - strand, within Pf6N2E2_5872 at 6692.755 kb on + strand, within Pf6N2E2_5872 at 6692.755 kb on + strand, within Pf6N2E2_5872 at 6692.756 kb on - strand, within Pf6N2E2_5872 at 6692.756 kb on - strand, within Pf6N2E2_5872 at 6692.950 kb on + strand, within Pf6N2E2_5872 at 6692.950 kb on + strand, within Pf6N2E2_5872 at 6692.950 kb on + strand, within Pf6N2E2_5872 at 6692.951 kb on - strand, within Pf6N2E2_5872 at 6692.951 kb on - strand, within Pf6N2E2_5872 at 6693.091 kb on + strand, within Pf6N2E2_5872 at 6693.092 kb on - strand, within Pf6N2E2_5872 at 6693.092 kb on - strand, within Pf6N2E2_5872 at 6693.574 kb on + strand, within Pf6N2E2_5873 at 6693.576 kb on + strand, within Pf6N2E2_5873 at 6693.576 kb on + strand, within Pf6N2E2_5873 at 6693.576 kb on + strand, within Pf6N2E2_5873 at 6693.576 kb on + strand, within Pf6N2E2_5873 at 6693.576 kb on + strand, within Pf6N2E2_5873 at 6693.576 kb on + strand, within Pf6N2E2_5873 at 6693.576 kb on + strand, within Pf6N2E2_5873 at 6693.577 kb on - strand, within Pf6N2E2_5873 at 6693.577 kb on - strand, within Pf6N2E2_5873 at 6693.577 kb on - strand, within Pf6N2E2_5873 at 6693.577 kb on - strand, within Pf6N2E2_5873 at 6693.577 kb on - strand, within Pf6N2E2_5873 at 6693.646 kb on - strand, within Pf6N2E2_5873 at 6693.646 kb on - strand, within Pf6N2E2_5873 at 6693.684 kb on + strand, within Pf6N2E2_5873 at 6693.684 kb on + strand, within Pf6N2E2_5873 at 6693.684 kb on + strand, within Pf6N2E2_5873 at 6693.684 kb on + strand, within Pf6N2E2_5873 at 6693.684 kb on + strand, within Pf6N2E2_5873 at 6693.684 kb on + strand, within Pf6N2E2_5873 at 6693.685 kb on - strand, within Pf6N2E2_5873 at 6693.685 kb on - strand, within Pf6N2E2_5873 at 6693.849 kb on + strand, within Pf6N2E2_5873 at 6693.849 kb on + strand, within Pf6N2E2_5873 at 6693.850 kb on - strand, within Pf6N2E2_5873 at 6693.850 kb on - strand, within Pf6N2E2_5873 at 6693.869 kb on + strand, within Pf6N2E2_5873 at 6693.869 kb on + strand, within Pf6N2E2_5873 at 6694.259 kb on + strand at 6694.259 kb on + strand at 6694.259 kb on + strand at 6694.260 kb on - strand at 6694.260 kb on - strand at 6694.260 kb on - strand at 6694.260 kb on - strand at 6694.260 kb on - strand at 6694.317 kb on + strand at 6694.317 kb on + strand at 6694.318 kb on - strand at 6694.318 kb on - strand at 6694.318 kb on - strand at 6694.341 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 6,691,374 - -0.1 6,691,374 - +0.3 6,691,374 - -1.7 6,691,430 - +2.0 6,691,486 + -3.9 6,691,486 + -0.9 6,691,486 + -1.5 6,691,487 - +1.2 6,691,487 - +1.2 6,691,487 - +0.4 6,691,547 - +3.9 6,691,547 - +2.7 6,691,734 + Pf6N2E2_5872 0.13 +1.9 6,691,734 + Pf6N2E2_5872 0.13 +2.4 6,691,734 + Pf6N2E2_5872 0.13 +4.2 6,691,734 + Pf6N2E2_5872 0.13 +2.6 6,691,735 - Pf6N2E2_5872 0.13 +3.8 6,691,836 + Pf6N2E2_5872 0.18 +1.7 6,691,836 + Pf6N2E2_5872 0.18 +2.4 6,691,836 + Pf6N2E2_5872 0.18 +1.2 6,691,837 - Pf6N2E2_5872 0.19 +1.5 6,691,837 - Pf6N2E2_5872 0.19 +0.9 6,691,837 - Pf6N2E2_5872 0.19 +0.2 6,691,837 - Pf6N2E2_5872 0.19 +0.0 6,691,837 - Pf6N2E2_5872 0.19 -0.4 6,691,837 - Pf6N2E2_5872 0.19 +1.5 6,691,837 - Pf6N2E2_5872 0.19 -0.9 6,691,837 - Pf6N2E2_5872 0.19 +0.8 6,691,837 - Pf6N2E2_5872 0.19 +0.4 6,692,482 + Pf6N2E2_5872 0.52 +4.4 6,692,482 + Pf6N2E2_5872 0.52 +4.5 6,692,482 + Pf6N2E2_5872 0.52 +5.0 6,692,482 + Pf6N2E2_5872 0.52 +4.5 6,692,483 - Pf6N2E2_5872 0.52 +1.8 6,692,755 + Pf6N2E2_5872 0.67 +4.9 6,692,755 + Pf6N2E2_5872 0.67 +4.5 6,692,756 - Pf6N2E2_5872 0.67 +4.4 6,692,756 - Pf6N2E2_5872 0.67 +4.6 6,692,950 + Pf6N2E2_5872 0.77 +4.2 6,692,950 + Pf6N2E2_5872 0.77 -0.2 6,692,950 + Pf6N2E2_5872 0.77 +4.4 6,692,951 - Pf6N2E2_5872 0.77 +4.4 6,692,951 - Pf6N2E2_5872 0.77 +3.4 6,693,091 + Pf6N2E2_5872 0.84 +3.0 6,693,092 - Pf6N2E2_5872 0.84 +4.9 6,693,092 - Pf6N2E2_5872 0.84 +2.7 6,693,574 + Pf6N2E2_5873 0.20 +2.7 6,693,576 + Pf6N2E2_5873 0.21 +3.5 6,693,576 + Pf6N2E2_5873 0.21 +3.4 6,693,576 + Pf6N2E2_5873 0.21 +3.0 6,693,576 + Pf6N2E2_5873 0.21 +2.9 6,693,576 + Pf6N2E2_5873 0.21 +2.4 6,693,576 + Pf6N2E2_5873 0.21 +3.0 6,693,576 + Pf6N2E2_5873 0.21 +3.1 6,693,577 - Pf6N2E2_5873 0.21 -0.7 6,693,577 - Pf6N2E2_5873 0.21 -1.8 6,693,577 - Pf6N2E2_5873 0.21 -1.8 6,693,577 - Pf6N2E2_5873 0.21 -0.1 6,693,577 - Pf6N2E2_5873 0.21 -1.4 6,693,646 - Pf6N2E2_5873 0.28 -0.8 6,693,646 - Pf6N2E2_5873 0.28 -1.9 6,693,684 + Pf6N2E2_5873 0.32 +3.4 6,693,684 + Pf6N2E2_5873 0.32 +2.4 6,693,684 + Pf6N2E2_5873 0.32 +2.4 6,693,684 + Pf6N2E2_5873 0.32 +3.2 6,693,684 + Pf6N2E2_5873 0.32 +3.0 6,693,684 + Pf6N2E2_5873 0.32 +3.2 6,693,685 - Pf6N2E2_5873 0.32 +0.9 6,693,685 - Pf6N2E2_5873 0.32 -1.3 6,693,849 + Pf6N2E2_5873 0.50 +4.7 6,693,849 + Pf6N2E2_5873 0.50 +3.0 6,693,850 - Pf6N2E2_5873 0.50 +0.4 6,693,850 - Pf6N2E2_5873 0.50 -1.8 6,693,869 + Pf6N2E2_5873 0.52 +1.6 6,693,869 + Pf6N2E2_5873 0.52 +3.7 6,694,259 + +3.3 6,694,259 + -0.6 6,694,259 + +2.6 6,694,260 - -1.0 6,694,260 - -0.5 6,694,260 - -1.5 6,694,260 - +0.6 6,694,260 - +0.3 6,694,317 + +4.7 6,694,317 + +5.1 6,694,318 - -0.4 6,694,318 - +1.6 6,694,318 - -1.4 6,694,341 + +5.3
Or see this region's nucleotide sequence