Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_583 and Pf6N2E2_584 are separated by 4 nucleotides Pf6N2E2_584 and Pf6N2E2_585 overlap by 4 nucleotides
Pf6N2E2_583 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 658,464 to 659,537
_583
Pf6N2E2_584 - ABC transporter, periplasmic substrate-binding protein, at 659,542 to 661,395
_584
Pf6N2E2_585 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 661,392 to 663,206
_585
Position (kb) 659
660
661
662 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 658.741 kb on + strand, within Pf6N2E2_583 at 658.741 kb on + strand, within Pf6N2E2_583 at 658.742 kb on - strand, within Pf6N2E2_583 at 658.789 kb on + strand, within Pf6N2E2_583 at 658.790 kb on - strand, within Pf6N2E2_583 at 658.790 kb on - strand, within Pf6N2E2_583 at 658.790 kb on - strand, within Pf6N2E2_583 at 658.790 kb on - strand, within Pf6N2E2_583 at 658.894 kb on + strand, within Pf6N2E2_583 at 658.894 kb on + strand, within Pf6N2E2_583 at 658.894 kb on + strand, within Pf6N2E2_583 at 658.972 kb on + strand, within Pf6N2E2_583 at 658.972 kb on + strand, within Pf6N2E2_583 at 658.973 kb on - strand, within Pf6N2E2_583 at 659.122 kb on + strand, within Pf6N2E2_583 at 659.122 kb on + strand, within Pf6N2E2_583 at 659.122 kb on + strand, within Pf6N2E2_583 at 659.122 kb on + strand, within Pf6N2E2_583 at 659.123 kb on - strand, within Pf6N2E2_583 at 659.226 kb on + strand, within Pf6N2E2_583 at 659.227 kb on - strand, within Pf6N2E2_583 at 659.227 kb on - strand, within Pf6N2E2_583 at 659.236 kb on + strand, within Pf6N2E2_583 at 659.236 kb on + strand, within Pf6N2E2_583 at 659.287 kb on + strand, within Pf6N2E2_583 at 659.287 kb on + strand, within Pf6N2E2_583 at 659.288 kb on - strand, within Pf6N2E2_583 at 659.288 kb on - strand, within Pf6N2E2_583 at 659.291 kb on + strand, within Pf6N2E2_583 at 659.291 kb on + strand, within Pf6N2E2_583 at 659.291 kb on + strand, within Pf6N2E2_583 at 659.291 kb on + strand, within Pf6N2E2_583 at 659.291 kb on + strand, within Pf6N2E2_583 at 659.292 kb on - strand, within Pf6N2E2_583 at 659.292 kb on - strand, within Pf6N2E2_583 at 659.292 kb on - strand, within Pf6N2E2_583 at 659.525 kb on + strand at 659.525 kb on + strand at 659.525 kb on + strand at 659.526 kb on - strand at 659.527 kb on + strand at 659.528 kb on - strand at 659.593 kb on + strand at 659.593 kb on + strand at 659.593 kb on + strand at 659.593 kb on + strand at 659.594 kb on - strand at 659.594 kb on - strand at 659.651 kb on + strand at 659.652 kb on - strand at 659.732 kb on + strand, within Pf6N2E2_584 at 659.732 kb on + strand, within Pf6N2E2_584 at 659.733 kb on - strand, within Pf6N2E2_584 at 659.733 kb on - strand, within Pf6N2E2_584 at 659.733 kb on - strand at 659.733 kb on - strand, within Pf6N2E2_584 at 659.733 kb on - strand, within Pf6N2E2_584 at 659.733 kb on - strand, within Pf6N2E2_584 at 659.736 kb on - strand, within Pf6N2E2_584 at 659.776 kb on + strand, within Pf6N2E2_584 at 660.364 kb on + strand, within Pf6N2E2_584 at 660.364 kb on + strand, within Pf6N2E2_584 at 660.365 kb on - strand, within Pf6N2E2_584 at 660.365 kb on - strand, within Pf6N2E2_584 at 660.365 kb on - strand, within Pf6N2E2_584 at 660.421 kb on + strand, within Pf6N2E2_584 at 660.452 kb on + strand, within Pf6N2E2_584 at 660.453 kb on - strand, within Pf6N2E2_584 at 660.536 kb on + strand, within Pf6N2E2_584 at 660.536 kb on + strand, within Pf6N2E2_584 at 660.629 kb on + strand, within Pf6N2E2_584 at 660.629 kb on + strand, within Pf6N2E2_584 at 660.629 kb on + strand, within Pf6N2E2_584 at 660.630 kb on - strand, within Pf6N2E2_584 at 660.630 kb on - strand, within Pf6N2E2_584 at 660.630 kb on - strand, within Pf6N2E2_584 at 660.630 kb on - strand, within Pf6N2E2_584 at 660.644 kb on - strand, within Pf6N2E2_584 at 660.654 kb on + strand, within Pf6N2E2_584 at 660.692 kb on + strand, within Pf6N2E2_584 at 660.692 kb on + strand, within Pf6N2E2_584 at 660.692 kb on + strand, within Pf6N2E2_584 at 660.692 kb on + strand, within Pf6N2E2_584 at 660.693 kb on - strand, within Pf6N2E2_584 at 660.693 kb on - strand, within Pf6N2E2_584 at 660.700 kb on + strand, within Pf6N2E2_584 at 660.861 kb on + strand, within Pf6N2E2_584 at 660.861 kb on + strand, within Pf6N2E2_584 at 660.929 kb on + strand, within Pf6N2E2_584 at 660.933 kb on - strand, within Pf6N2E2_584 at 661.041 kb on - strand, within Pf6N2E2_584 at 661.041 kb on - strand, within Pf6N2E2_584 at 661.097 kb on + strand, within Pf6N2E2_584 at 661.142 kb on + strand, within Pf6N2E2_584 at 661.304 kb on + strand at 661.304 kb on + strand at 661.305 kb on - strand at 661.444 kb on + strand at 661.444 kb on + strand at 661.444 kb on + strand at 661.445 kb on - strand at 661.481 kb on - strand at 661.495 kb on + strand at 661.495 kb on + strand at 661.496 kb on - strand at 661.496 kb on - strand at 661.504 kb on + strand at 661.522 kb on + strand at 661.523 kb on - strand at 661.643 kb on - strand, within Pf6N2E2_585 at 661.643 kb on - strand, within Pf6N2E2_585 at 661.685 kb on - strand, within Pf6N2E2_585 at 661.771 kb on + strand, within Pf6N2E2_585 at 661.772 kb on - strand, within Pf6N2E2_585 at 661.772 kb on - strand, within Pf6N2E2_585 at 661.834 kb on + strand, within Pf6N2E2_585 at 661.834 kb on + strand, within Pf6N2E2_585 at 661.835 kb on - strand, within Pf6N2E2_585 at 661.835 kb on - strand, within Pf6N2E2_585 at 661.835 kb on - strand, within Pf6N2E2_585 at 661.835 kb on - strand, within Pf6N2E2_585 at 662.122 kb on + strand, within Pf6N2E2_585 at 662.123 kb on - strand, within Pf6N2E2_585 at 662.123 kb on - strand, within Pf6N2E2_585 at 662.140 kb on + strand, within Pf6N2E2_585 at 662.141 kb on - strand, within Pf6N2E2_585 at 662.141 kb on - strand, within Pf6N2E2_585 at 662.205 kb on + strand, within Pf6N2E2_585 at 662.206 kb on - strand, within Pf6N2E2_585 at 662.295 kb on + strand, within Pf6N2E2_585 at 662.295 kb on + strand, within Pf6N2E2_585 at 662.296 kb on - strand, within Pf6N2E2_585 at 662.296 kb on - strand, within Pf6N2E2_585 at 662.362 kb on + strand, within Pf6N2E2_585 at 662.362 kb on + strand, within Pf6N2E2_585 at 662.362 kb on + strand, within Pf6N2E2_585
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 658,741 + Pf6N2E2_583 0.26 -0.6 658,741 + Pf6N2E2_583 0.26 -1.2 658,742 - Pf6N2E2_583 0.26 -0.8 658,789 + Pf6N2E2_583 0.30 -2.0 658,790 - Pf6N2E2_583 0.30 -0.0 658,790 - Pf6N2E2_583 0.30 -3.5 658,790 - Pf6N2E2_583 0.30 -2.5 658,790 - Pf6N2E2_583 0.30 -0.8 658,894 + Pf6N2E2_583 0.40 -1.7 658,894 + Pf6N2E2_583 0.40 -0.3 658,894 + Pf6N2E2_583 0.40 -1.7 658,972 + Pf6N2E2_583 0.47 -0.8 658,972 + Pf6N2E2_583 0.47 -1.9 658,973 - Pf6N2E2_583 0.47 -1.5 659,122 + Pf6N2E2_583 0.61 -0.6 659,122 + Pf6N2E2_583 0.61 -1.1 659,122 + Pf6N2E2_583 0.61 -1.1 659,122 + Pf6N2E2_583 0.61 -2.2 659,123 - Pf6N2E2_583 0.61 -2.1 659,226 + Pf6N2E2_583 0.71 -2.2 659,227 - Pf6N2E2_583 0.71 -3.1 659,227 - Pf6N2E2_583 0.71 -1.4 659,236 + Pf6N2E2_583 0.72 -1.3 659,236 + Pf6N2E2_583 0.72 +0.2 659,287 + Pf6N2E2_583 0.77 -1.8 659,287 + Pf6N2E2_583 0.77 -1.0 659,288 - Pf6N2E2_583 0.77 -0.4 659,288 - Pf6N2E2_583 0.77 -0.2 659,291 + Pf6N2E2_583 0.77 -1.9 659,291 + Pf6N2E2_583 0.77 -2.6 659,291 + Pf6N2E2_583 0.77 -3.0 659,291 + Pf6N2E2_583 0.77 -2.2 659,291 + Pf6N2E2_583 0.77 -0.6 659,292 - Pf6N2E2_583 0.77 -0.5 659,292 - Pf6N2E2_583 0.77 -0.4 659,292 - Pf6N2E2_583 0.77 -1.7 659,525 + -1.4 659,525 + -2.5 659,525 + -1.2 659,526 - -0.7 659,527 + -0.7 659,528 - -0.3 659,593 + -1.6 659,593 + -1.3 659,593 + -1.2 659,593 + -3.1 659,594 - -1.3 659,594 - -0.9 659,651 + -2.0 659,652 - -0.4 659,732 + Pf6N2E2_584 0.10 -1.1 659,732 + Pf6N2E2_584 0.10 +0.1 659,733 - Pf6N2E2_584 0.10 -3.1 659,733 - Pf6N2E2_584 0.10 +1.1 659,733 - +1.9 659,733 - Pf6N2E2_584 0.10 -2.4 659,733 - Pf6N2E2_584 0.10 -2.1 659,733 - Pf6N2E2_584 0.10 -1.9 659,736 - Pf6N2E2_584 0.10 -1.4 659,776 + Pf6N2E2_584 0.13 -2.0 660,364 + Pf6N2E2_584 0.44 -2.2 660,364 + Pf6N2E2_584 0.44 -0.1 660,365 - Pf6N2E2_584 0.44 -0.4 660,365 - Pf6N2E2_584 0.44 -1.8 660,365 - Pf6N2E2_584 0.44 -0.6 660,421 + Pf6N2E2_584 0.47 -1.5 660,452 + Pf6N2E2_584 0.49 +1.5 660,453 - Pf6N2E2_584 0.49 -1.8 660,536 + Pf6N2E2_584 0.54 -1.9 660,536 + Pf6N2E2_584 0.54 -1.3 660,629 + Pf6N2E2_584 0.59 -3.2 660,629 + Pf6N2E2_584 0.59 -1.0 660,629 + Pf6N2E2_584 0.59 -1.4 660,630 - Pf6N2E2_584 0.59 -0.5 660,630 - Pf6N2E2_584 0.59 -1.3 660,630 - Pf6N2E2_584 0.59 -0.8 660,630 - Pf6N2E2_584 0.59 -2.1 660,644 - Pf6N2E2_584 0.59 -0.6 660,654 + Pf6N2E2_584 0.60 -2.2 660,692 + Pf6N2E2_584 0.62 -0.9 660,692 + Pf6N2E2_584 0.62 -0.9 660,692 + Pf6N2E2_584 0.62 -2.2 660,692 + Pf6N2E2_584 0.62 -2.1 660,693 - Pf6N2E2_584 0.62 -2.5 660,693 - Pf6N2E2_584 0.62 +0.3 660,700 + Pf6N2E2_584 0.62 -0.7 660,861 + Pf6N2E2_584 0.71 -1.6 660,861 + Pf6N2E2_584 0.71 +0.1 660,929 + Pf6N2E2_584 0.75 -1.9 660,933 - Pf6N2E2_584 0.75 -0.7 661,041 - Pf6N2E2_584 0.81 -0.2 661,041 - Pf6N2E2_584 0.81 -0.7 661,097 + Pf6N2E2_584 0.84 -0.7 661,142 + Pf6N2E2_584 0.86 +0.3 661,304 + +2.4 661,304 + -1.7 661,305 - -3.0 661,444 + -2.0 661,444 + -0.5 661,444 + -0.2 661,445 - -0.4 661,481 - +0.7 661,495 + +0.3 661,495 + -1.9 661,496 - +1.1 661,496 - +0.3 661,504 + -0.3 661,522 + -0.5 661,523 - +1.3 661,643 - Pf6N2E2_585 0.14 +0.7 661,643 - Pf6N2E2_585 0.14 +0.8 661,685 - Pf6N2E2_585 0.16 +1.1 661,771 + Pf6N2E2_585 0.21 +1.4 661,772 - Pf6N2E2_585 0.21 +0.4 661,772 - Pf6N2E2_585 0.21 +0.8 661,834 + Pf6N2E2_585 0.24 -3.0 661,834 + Pf6N2E2_585 0.24 -1.4 661,835 - Pf6N2E2_585 0.24 +1.3 661,835 - Pf6N2E2_585 0.24 +2.7 661,835 - Pf6N2E2_585 0.24 +0.9 661,835 - Pf6N2E2_585 0.24 +1.9 662,122 + Pf6N2E2_585 0.40 -0.2 662,123 - Pf6N2E2_585 0.40 +1.3 662,123 - Pf6N2E2_585 0.40 -1.9 662,140 + Pf6N2E2_585 0.41 +1.0 662,141 - Pf6N2E2_585 0.41 -0.8 662,141 - Pf6N2E2_585 0.41 +0.5 662,205 + Pf6N2E2_585 0.45 -1.8 662,206 - Pf6N2E2_585 0.45 +0.5 662,295 + Pf6N2E2_585 0.50 +0.5 662,295 + Pf6N2E2_585 0.50 -0.3 662,296 - Pf6N2E2_585 0.50 -0.3 662,296 - Pf6N2E2_585 0.50 -1.4 662,362 + Pf6N2E2_585 0.53 -1.7 662,362 + Pf6N2E2_585 0.53 -2.1 662,362 + Pf6N2E2_585 0.53 -0.5
Or see this region's nucleotide sequence