Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_54 and Pf6N2E2_55 are separated by 28 nucleotides Pf6N2E2_55 and Pf6N2E2_56 are separated by 45 nucleotides Pf6N2E2_56 and Pf6N2E2_57 overlap by 43 nucleotides Pf6N2E2_57 and Pf6N2E2_58 overlap by 6 nucleotides
Pf6N2E2_54 - Transcriptional regulator, TetR family, at 94,876 to 95,415
_54
Pf6N2E2_55 - Probable hydrolase, at 95,444 to 96,277
_55
Pf6N2E2_56 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 96,323 to 96,772
_56
Pf6N2E2_57 - hypothetical protein, at 96,730 to 96,891
_57
Pf6N2E2_58 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 96,886 to 97,845
_58
Position (kb) 96
97 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 95.418 kb on + strand at 95.419 kb on - strand at 95.419 kb on - strand at 95.468 kb on - strand at 95.626 kb on - strand, within Pf6N2E2_55 at 95.626 kb on - strand, within Pf6N2E2_55 at 95.724 kb on - strand, within Pf6N2E2_55 at 95.724 kb on - strand, within Pf6N2E2_55 at 96.352 kb on + strand at 96.367 kb on - strand at 96.411 kb on + strand, within Pf6N2E2_56 at 96.412 kb on - strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.543 kb on - strand, within Pf6N2E2_56 at 96.543 kb on - strand, within Pf6N2E2_56 at 96.706 kb on + strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 97.377 kb on + strand, within Pf6N2E2_58 at 97.411 kb on + strand, within Pf6N2E2_58 at 97.411 kb on + strand, within Pf6N2E2_58
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 95,418 + +0.6 95,419 - -0.1 95,419 - +1.5 95,468 - +0.7 95,626 - Pf6N2E2_55 0.22 +0.0 95,626 - Pf6N2E2_55 0.22 -0.4 95,724 - Pf6N2E2_55 0.34 +0.6 95,724 - Pf6N2E2_55 0.34 -0.4 96,352 + -0.1 96,367 - +0.1 96,411 + Pf6N2E2_56 0.20 -0.4 96,412 - Pf6N2E2_56 0.20 +1.3 96,413 + Pf6N2E2_56 0.20 -2.9 96,413 + Pf6N2E2_56 0.20 +0.5 96,413 + Pf6N2E2_56 0.20 -0.3 96,413 + Pf6N2E2_56 0.20 -2.3 96,413 + Pf6N2E2_56 0.20 -0.8 96,413 + Pf6N2E2_56 0.20 +0.0 96,413 + Pf6N2E2_56 0.20 +0.2 96,413 + Pf6N2E2_56 0.20 +1.0 96,414 - Pf6N2E2_56 0.20 +2.0 96,414 - +2.5 96,414 - Pf6N2E2_56 0.20 +0.3 96,414 - Pf6N2E2_56 0.20 -1.8 96,414 - Pf6N2E2_56 0.20 +0.8 96,543 - Pf6N2E2_56 0.49 -0.6 96,543 - Pf6N2E2_56 0.49 +2.1 96,706 + Pf6N2E2_56 0.85 +0.7 96,707 - Pf6N2E2_56 0.85 -2.5 96,707 - Pf6N2E2_56 0.85 -0.7 96,707 - Pf6N2E2_56 0.85 +0.6 97,377 + Pf6N2E2_58 0.51 -0.9 97,411 + Pf6N2E2_58 0.55 -2.7 97,411 + Pf6N2E2_58 0.55 -1.0
Or see this region's nucleotide sequence