Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5391 and Pf6N2E2_5392 are separated by 168 nucleotides Pf6N2E2_5392 and Pf6N2E2_5393 overlap by 4 nucleotides Pf6N2E2_5393 and Pf6N2E2_5394 are separated by 3 nucleotides Pf6N2E2_5394 and Pf6N2E2_5395 are separated by 298 nucleotides
Pf6N2E2_5391 - Predicted ATPase related to phosphate starvation-inducible protein PhoH, at 6,197,912 to 6,199,306
_5391
Pf6N2E2_5392 - Molybdenum cofactor biosynthesis protein MoaC, at 6,199,475 to 6,199,948
_5392
Pf6N2E2_5393 - Molybdenum cofactor biosynthesis protein MoaD, at 6,199,945 to 6,200,187
_5393
Pf6N2E2_5394 - Molybdenum cofactor biosynthesis protein MoaE, at 6,200,191 to 6,200,643
_5394
Pf6N2E2_5395 - Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein, at 6,200,942 to 6,201,676
_5395
Position (kb)
6199
6200 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6198.689 kb on + strand, within Pf6N2E2_5391 at 6198.689 kb on + strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6199.025 kb on + strand, within Pf6N2E2_5391 at 6199.025 kb on + strand, within Pf6N2E2_5391 at 6199.025 kb on + strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.168 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.205 kb on + strand at 6199.205 kb on + strand at 6199.205 kb on + strand at 6199.205 kb on + strand at 6199.206 kb on - strand at 6199.206 kb on - strand at 6199.332 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.353 kb on + strand at 6199.353 kb on + strand at 6199.354 kb on - strand at 6199.354 kb on - strand at 6199.354 kb on - strand at 6199.435 kb on + strand at 6199.435 kb on + strand at 6199.443 kb on + strand at 6199.443 kb on + strand at 6199.444 kb on - strand at 6199.469 kb on - strand at 6199.755 kb on + strand, within Pf6N2E2_5392 at 6199.844 kb on + strand, within Pf6N2E2_5392 at 6199.844 kb on + strand, within Pf6N2E2_5392 at 6199.844 kb on + strand, within Pf6N2E2_5392 at 6199.845 kb on - strand, within Pf6N2E2_5392 at 6199.845 kb on - strand, within Pf6N2E2_5392 at 6200.533 kb on + strand, within Pf6N2E2_5394 at 6200.533 kb on + strand, within Pf6N2E2_5394 at 6200.534 kb on - strand, within Pf6N2E2_5394 at 6200.534 kb on - strand, within Pf6N2E2_5394 at 6200.641 kb on + strand at 6200.641 kb on + strand at 6200.642 kb on - strand at 6200.642 kb on - strand at 6200.719 kb on + strand at 6200.719 kb on + strand at 6200.719 kb on + strand at 6200.719 kb on + strand at 6200.719 kb on + strand at 6200.720 kb on - strand at 6200.720 kb on - strand at 6200.720 kb on - strand at 6200.720 kb on - strand at 6200.720 kb on - strand at 6200.720 kb on - strand at 6200.832 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 6,198,689 + Pf6N2E2_5391 0.56 +1.0 6,198,689 + Pf6N2E2_5391 0.56 +0.4 6,198,690 - Pf6N2E2_5391 0.56 +0.1 6,198,690 - Pf6N2E2_5391 0.56 +0.3 6,198,690 - Pf6N2E2_5391 0.56 -0.4 6,198,690 - Pf6N2E2_5391 0.56 +0.6 6,198,690 - Pf6N2E2_5391 0.56 -0.4 6,198,690 - Pf6N2E2_5391 0.56 +0.7 6,198,983 + Pf6N2E2_5391 0.77 +0.8 6,198,983 + Pf6N2E2_5391 0.77 -1.4 6,198,983 + Pf6N2E2_5391 0.77 -1.9 6,198,983 + Pf6N2E2_5391 0.77 +0.3 6,198,983 + Pf6N2E2_5391 0.77 -0.3 6,198,983 + Pf6N2E2_5391 0.77 +0.7 6,198,984 - Pf6N2E2_5391 0.77 +0.0 6,198,984 - Pf6N2E2_5391 0.77 +0.9 6,198,984 - Pf6N2E2_5391 0.77 -0.1 6,198,984 - Pf6N2E2_5391 0.77 +0.8 6,198,984 - Pf6N2E2_5391 0.77 -0.0 6,198,984 - Pf6N2E2_5391 0.77 +0.6 6,199,025 + Pf6N2E2_5391 0.80 +0.5 6,199,025 + Pf6N2E2_5391 0.80 -0.8 6,199,025 + Pf6N2E2_5391 0.80 +1.0 6,199,026 - Pf6N2E2_5391 0.80 -1.3 6,199,026 - Pf6N2E2_5391 0.80 -2.1 6,199,026 - Pf6N2E2_5391 0.80 -2.4 6,199,026 - Pf6N2E2_5391 0.80 -0.1 6,199,026 - Pf6N2E2_5391 0.80 -0.0 6,199,026 - Pf6N2E2_5391 0.80 -1.0 6,199,168 + +0.3 6,199,175 + -0.0 6,199,175 + +0.1 6,199,175 + -0.6 6,199,175 + +0.0 6,199,175 + +0.8 6,199,175 + -3.1 6,199,175 + +1.3 6,199,176 - -1.7 6,199,176 - +0.5 6,199,176 - +0.1 6,199,176 - +1.6 6,199,176 - +0.0 6,199,205 + +1.3 6,199,205 + +0.1 6,199,205 + -1.7 6,199,205 + -0.1 6,199,206 - +0.5 6,199,206 - -0.2 6,199,332 - -0.1 6,199,347 - +0.0 6,199,347 - -1.3 6,199,347 - +0.7 6,199,347 - -0.1 6,199,347 - -0.1 6,199,353 + -0.5 6,199,353 + -0.2 6,199,354 - +0.7 6,199,354 - -2.4 6,199,354 - -1.1 6,199,435 + -1.3 6,199,435 + +0.6 6,199,443 + -0.3 6,199,443 + +0.3 6,199,444 - -0.8 6,199,469 - -2.1 6,199,755 + Pf6N2E2_5392 0.59 -0.6 6,199,844 + Pf6N2E2_5392 0.78 -0.8 6,199,844 + Pf6N2E2_5392 0.78 -1.7 6,199,844 + Pf6N2E2_5392 0.78 -1.6 6,199,845 - Pf6N2E2_5392 0.78 -1.7 6,199,845 - Pf6N2E2_5392 0.78 -1.6 6,200,533 + Pf6N2E2_5394 0.75 +0.5 6,200,533 + Pf6N2E2_5394 0.75 -1.0 6,200,534 - Pf6N2E2_5394 0.76 -0.6 6,200,534 - Pf6N2E2_5394 0.76 +0.6 6,200,641 + +1.1 6,200,641 + +0.8 6,200,642 - +0.7 6,200,642 - +0.4 6,200,719 + +0.3 6,200,719 + +2.1 6,200,719 + -0.9 6,200,719 + -0.3 6,200,719 + +0.3 6,200,720 - +0.5 6,200,720 - -0.3 6,200,720 - +0.1 6,200,720 - -0.2 6,200,720 - +0.1 6,200,720 - +1.3 6,200,832 + -1.4
Or see this region's nucleotide sequence