Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5345 and Pf6N2E2_5346 are separated by 341 nucleotides Pf6N2E2_5346 and Pf6N2E2_5347 are separated by 868 nucleotides
Pf6N2E2_5345 - LppC putative lipoprotein, at 6,138,063 to 6,139,874
_5345
Pf6N2E2_5346 - rRNA small subunit methyltransferase I, at 6,140,216 to 6,141,088
_5346
Pf6N2E2_5347 - Cell division protein MraZ, at 6,141,957 to 6,142,280
_5347
Position (kb)
6140
6141
6142 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6139.876 kb on + strand at 6139.876 kb on + strand at 6139.876 kb on + strand at 6139.876 kb on + strand at 6139.876 kb on + strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.877 kb on - strand at 6139.902 kb on + strand at 6139.916 kb on - strand at 6139.916 kb on - strand at 6139.944 kb on - strand at 6139.965 kb on + strand at 6139.966 kb on - strand at 6139.966 kb on - strand at 6140.069 kb on + strand at 6140.069 kb on + strand at 6140.069 kb on + strand at 6140.070 kb on - strand at 6140.098 kb on + strand at 6140.179 kb on + strand at 6140.179 kb on + strand at 6140.179 kb on + strand at 6140.194 kb on + strand at 6140.208 kb on + strand at 6140.208 kb on + strand at 6140.209 kb on - strand at 6140.258 kb on + strand at 6140.258 kb on + strand at 6140.258 kb on + strand at 6140.259 kb on - strand at 6140.259 kb on - strand at 6140.294 kb on - strand at 6140.689 kb on + strand, within Pf6N2E2_5346 at 6140.689 kb on + strand, within Pf6N2E2_5346 at 6140.689 kb on + strand, within Pf6N2E2_5346 at 6140.690 kb on - strand, within Pf6N2E2_5346 at 6140.721 kb on - strand, within Pf6N2E2_5346 at 6140.979 kb on + strand, within Pf6N2E2_5346 at 6140.980 kb on - strand, within Pf6N2E2_5346 at 6141.120 kb on - strand at 6141.558 kb on - strand at 6141.560 kb on + strand at 6141.561 kb on - strand at 6141.593 kb on + strand at 6141.693 kb on + strand at 6141.693 kb on + strand at 6141.744 kb on + strand at 6141.744 kb on + strand at 6141.744 kb on + strand at 6141.744 kb on + strand at 6141.744 kb on + strand at 6141.746 kb on + strand at 6141.925 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 6,139,876 + +0.8 6,139,876 + -0.1 6,139,876 + -1.0 6,139,876 + -0.2 6,139,876 + +1.5 6,139,877 - -0.5 6,139,877 - -0.7 6,139,877 - -0.0 6,139,877 - +0.2 6,139,877 - -0.4 6,139,877 - -1.0 6,139,877 - -1.2 6,139,877 - -0.3 6,139,877 - +1.5 6,139,877 - -2.8 6,139,877 - +1.6 6,139,877 - +0.3 6,139,877 - +0.4 6,139,902 + -2.0 6,139,916 - +1.5 6,139,916 - +1.2 6,139,944 - +0.9 6,139,965 + -0.5 6,139,966 - -0.7 6,139,966 - +1.2 6,140,069 + -0.5 6,140,069 + -1.0 6,140,069 + +0.2 6,140,070 - +1.9 6,140,098 + -1.4 6,140,179 + +1.2 6,140,179 + -0.8 6,140,179 + +2.9 6,140,194 + -0.9 6,140,208 + -0.0 6,140,208 + -1.1 6,140,209 - +0.8 6,140,258 + -0.8 6,140,258 + -1.0 6,140,258 + -2.7 6,140,259 - +0.0 6,140,259 - +1.2 6,140,294 - -2.6 6,140,689 + Pf6N2E2_5346 0.54 -2.2 6,140,689 + Pf6N2E2_5346 0.54 -1.4 6,140,689 + Pf6N2E2_5346 0.54 -1.4 6,140,690 - Pf6N2E2_5346 0.54 +1.0 6,140,721 - Pf6N2E2_5346 0.58 -1.4 6,140,979 + Pf6N2E2_5346 0.87 -0.4 6,140,980 - Pf6N2E2_5346 0.88 -2.3 6,141,120 - +1.9 6,141,558 - -0.2 6,141,560 + -1.3 6,141,561 - -1.6 6,141,593 + +0.8 6,141,693 + -0.6 6,141,693 + -2.7 6,141,744 + -1.1 6,141,744 + -0.2 6,141,744 + +1.9 6,141,744 + -2.2 6,141,744 + -2.1 6,141,746 + -1.2 6,141,925 + +0.1
Or see this region's nucleotide sequence