Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5058 and Pf6N2E2_5059 are separated by 103 nucleotides Pf6N2E2_5059 and Pf6N2E2_5060 are separated by 57 nucleotides
Pf6N2E2_5058 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 5,832,424 to 5,834,325
_5058
Pf6N2E2_5059 - Transcriptional regulator, TetR family, at 5,834,429 to 5,835,106
_5059
Pf6N2E2_5060 - hypothetical protein, at 5,835,164 to 5,836,111
_5060
Position (kb)
5834
5835
5836 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 5833.468 kb on + strand, within Pf6N2E2_5058 at 5833.469 kb on - strand, within Pf6N2E2_5058 at 5833.469 kb on - strand, within Pf6N2E2_5058 at 5833.571 kb on - strand, within Pf6N2E2_5058 at 5833.598 kb on + strand, within Pf6N2E2_5058 at 5833.654 kb on + strand, within Pf6N2E2_5058 at 5833.700 kb on + strand, within Pf6N2E2_5058 at 5833.701 kb on - strand, within Pf6N2E2_5058 at 5833.701 kb on - strand, within Pf6N2E2_5058 at 5833.701 kb on - strand, within Pf6N2E2_5058 at 5833.796 kb on - strand, within Pf6N2E2_5058 at 5833.796 kb on - strand, within Pf6N2E2_5058 at 5833.859 kb on + strand, within Pf6N2E2_5058 at 5833.859 kb on + strand, within Pf6N2E2_5058 at 5833.890 kb on + strand, within Pf6N2E2_5058 at 5833.890 kb on + strand, within Pf6N2E2_5058 at 5833.891 kb on - strand, within Pf6N2E2_5058 at 5833.891 kb on - strand, within Pf6N2E2_5058 at 5833.892 kb on - strand, within Pf6N2E2_5058 at 5834.066 kb on - strand, within Pf6N2E2_5058 at 5834.066 kb on - strand, within Pf6N2E2_5058 at 5834.068 kb on + strand, within Pf6N2E2_5058 at 5834.140 kb on + strand at 5834.140 kb on + strand at 5834.141 kb on - strand at 5834.141 kb on - strand at 5834.141 kb on - strand at 5834.141 kb on - strand at 5834.141 kb on - strand at 5834.179 kb on + strand at 5834.180 kb on - strand at 5834.180 kb on - strand at 5834.180 kb on - strand at 5834.236 kb on + strand at 5834.335 kb on - strand at 5834.434 kb on + strand at 5834.434 kb on + strand at 5834.435 kb on - strand at 5834.624 kb on + strand, within Pf6N2E2_5059 at 5834.624 kb on + strand, within Pf6N2E2_5059 at 5834.624 kb on + strand, within Pf6N2E2_5059 at 5834.624 kb on + strand, within Pf6N2E2_5059 at 5834.624 kb on + strand, within Pf6N2E2_5059 at 5834.625 kb on - strand, within Pf6N2E2_5059 at 5834.625 kb on - strand, within Pf6N2E2_5059 at 5834.625 kb on - strand, within Pf6N2E2_5059 at 5834.625 kb on - strand, within Pf6N2E2_5059 at 5834.627 kb on + strand, within Pf6N2E2_5059 at 5834.627 kb on + strand, within Pf6N2E2_5059 at 5834.627 kb on + strand, within Pf6N2E2_5059 at 5834.627 kb on + strand, within Pf6N2E2_5059 at 5834.628 kb on - strand, within Pf6N2E2_5059 at 5834.630 kb on + strand, within Pf6N2E2_5059 at 5834.630 kb on + strand, within Pf6N2E2_5059 at 5834.630 kb on + strand, within Pf6N2E2_5059 at 5834.630 kb on + strand, within Pf6N2E2_5059 at 5834.631 kb on - strand, within Pf6N2E2_5059 at 5834.631 kb on - strand, within Pf6N2E2_5059 at 5834.631 kb on - strand, within Pf6N2E2_5059 at 5834.631 kb on - strand, within Pf6N2E2_5059 at 5834.654 kb on + strand, within Pf6N2E2_5059 at 5834.655 kb on - strand, within Pf6N2E2_5059 at 5834.655 kb on - strand, within Pf6N2E2_5059 at 5834.655 kb on - strand, within Pf6N2E2_5059 at 5834.678 kb on + strand, within Pf6N2E2_5059 at 5834.678 kb on + strand, within Pf6N2E2_5059 at 5834.834 kb on + strand, within Pf6N2E2_5059 at 5834.834 kb on + strand, within Pf6N2E2_5059 at 5834.835 kb on - strand, within Pf6N2E2_5059 at 5834.835 kb on - strand, within Pf6N2E2_5059 at 5834.873 kb on - strand, within Pf6N2E2_5059 at 5835.092 kb on + strand at 5835.093 kb on - strand at 5835.096 kb on + strand at 5835.097 kb on - strand at 5835.097 kb on - strand at 5835.097 kb on - strand at 5835.146 kb on - strand at 5835.146 kb on - strand at 5835.146 kb on - strand at 5835.146 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,833,468 + Pf6N2E2_5058 0.55 -2.3 5,833,469 - Pf6N2E2_5058 0.55 +1.1 5,833,469 - Pf6N2E2_5058 0.55 -0.8 5,833,571 - Pf6N2E2_5058 0.60 +1.5 5,833,598 + Pf6N2E2_5058 0.62 +0.3 5,833,654 + Pf6N2E2_5058 0.65 +1.2 5,833,700 + Pf6N2E2_5058 0.67 -0.3 5,833,701 - Pf6N2E2_5058 0.67 -0.4 5,833,701 - Pf6N2E2_5058 0.67 -0.2 5,833,701 - Pf6N2E2_5058 0.67 +0.8 5,833,796 - Pf6N2E2_5058 0.72 +1.8 5,833,796 - Pf6N2E2_5058 0.72 +0.2 5,833,859 + Pf6N2E2_5058 0.75 -2.2 5,833,859 + Pf6N2E2_5058 0.75 +0.6 5,833,890 + Pf6N2E2_5058 0.77 +0.4 5,833,890 + Pf6N2E2_5058 0.77 -2.4 5,833,891 - Pf6N2E2_5058 0.77 -1.1 5,833,891 - Pf6N2E2_5058 0.77 -0.6 5,833,892 - Pf6N2E2_5058 0.77 -0.5 5,834,066 - Pf6N2E2_5058 0.86 -1.4 5,834,066 - Pf6N2E2_5058 0.86 +1.1 5,834,068 + Pf6N2E2_5058 0.86 -1.1 5,834,140 + +0.5 5,834,140 + -0.6 5,834,141 - -0.7 5,834,141 - -1.3 5,834,141 - +0.2 5,834,141 - +0.4 5,834,141 - +1.7 5,834,179 + +0.5 5,834,180 - +0.7 5,834,180 - +0.3 5,834,180 - +0.6 5,834,236 + +0.0 5,834,335 - +1.9 5,834,434 + +1.9 5,834,434 + +1.0 5,834,435 - +0.2 5,834,624 + Pf6N2E2_5059 0.29 +0.4 5,834,624 + Pf6N2E2_5059 0.29 +0.6 5,834,624 + Pf6N2E2_5059 0.29 +1.1 5,834,624 + Pf6N2E2_5059 0.29 +0.8 5,834,624 + Pf6N2E2_5059 0.29 +2.0 5,834,625 - Pf6N2E2_5059 0.29 +2.3 5,834,625 - Pf6N2E2_5059 0.29 -0.0 5,834,625 - Pf6N2E2_5059 0.29 -1.3 5,834,625 - Pf6N2E2_5059 0.29 +2.3 5,834,627 + Pf6N2E2_5059 0.29 +2.0 5,834,627 + Pf6N2E2_5059 0.29 +1.0 5,834,627 + Pf6N2E2_5059 0.29 +2.4 5,834,627 + Pf6N2E2_5059 0.29 -0.8 5,834,628 - Pf6N2E2_5059 0.29 -1.2 5,834,630 + Pf6N2E2_5059 0.30 +1.5 5,834,630 + Pf6N2E2_5059 0.30 +0.2 5,834,630 + Pf6N2E2_5059 0.30 -0.3 5,834,630 + Pf6N2E2_5059 0.30 -1.8 5,834,631 - Pf6N2E2_5059 0.30 -1.1 5,834,631 - Pf6N2E2_5059 0.30 +1.8 5,834,631 - Pf6N2E2_5059 0.30 -0.1 5,834,631 - Pf6N2E2_5059 0.30 +1.3 5,834,654 + Pf6N2E2_5059 0.33 +1.3 5,834,655 - Pf6N2E2_5059 0.33 +2.0 5,834,655 - Pf6N2E2_5059 0.33 +0.5 5,834,655 - Pf6N2E2_5059 0.33 -0.3 5,834,678 + Pf6N2E2_5059 0.37 +1.6 5,834,678 + Pf6N2E2_5059 0.37 +2.2 5,834,834 + Pf6N2E2_5059 0.60 -0.5 5,834,834 + Pf6N2E2_5059 0.60 +1.0 5,834,835 - Pf6N2E2_5059 0.60 +1.9 5,834,835 - Pf6N2E2_5059 0.60 +1.0 5,834,873 - Pf6N2E2_5059 0.65 +3.4 5,835,092 + +0.9 5,835,093 - +1.4 5,835,096 + +1.5 5,835,097 - +2.5 5,835,097 - +4.9 5,835,097 - +1.5 5,835,146 - -0.0 5,835,146 - +0.9 5,835,146 - -0.8 5,835,146 - +0.6
Or see this region's nucleotide sequence