Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4780 and Pf6N2E2_4781 are separated by 301 nucleotides Pf6N2E2_4781 and Pf6N2E2_4782 are separated by 429 nucleotides Pf6N2E2_4782 and Pf6N2E2_4783 are separated by 412 nucleotides
Pf6N2E2_4780 - FIG00953270: hypothetical protein, at 5,522,329 to 5,524,692
_4780
Pf6N2E2_4781 - hypothetical protein, at 5,524,994 to 5,525,107
_4781
Pf6N2E2_4782 - hypothetical protein, at 5,525,537 to 5,527,108
_4782
Pf6N2E2_4783 - DNA-methyltransferase, at 5,527,521 to 5,528,036
_4783
Position (kb)
5525
5526
5527
5528 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5524.670 kb on - strand at 5524.802 kb on - strand at 5524.802 kb on - strand at 5524.814 kb on + strand at 5524.814 kb on + strand at 5524.815 kb on - strand at 5524.815 kb on - strand at 5524.817 kb on + strand at 5524.817 kb on + strand at 5524.817 kb on + strand at 5524.817 kb on + strand at 5524.817 kb on + strand at 5524.818 kb on - strand at 5524.818 kb on - strand at 5524.818 kb on - strand at 5524.818 kb on - strand at 5524.898 kb on + strand at 5524.898 kb on + strand at 5524.899 kb on - strand at 5524.899 kb on - strand at 5524.899 kb on - strand at 5524.950 kb on + strand at 5524.950 kb on + strand at 5524.951 kb on - strand at 5525.165 kb on - strand at 5525.165 kb on - strand at 5525.165 kb on - strand at 5525.193 kb on + strand at 5525.193 kb on + strand at 5525.194 kb on - strand at 5525.194 kb on - strand at 5525.194 kb on - strand at 5525.194 kb on - strand at 5525.194 kb on - strand at 5525.194 kb on - strand at 5525.209 kb on + strand at 5525.209 kb on + strand at 5525.210 kb on - strand at 5525.221 kb on + strand at 5525.237 kb on + strand at 5525.238 kb on - strand at 5525.238 kb on - strand at 5525.266 kb on - strand at 5525.302 kb on - strand at 5525.302 kb on - strand at 5525.302 kb on - strand at 5525.304 kb on + strand at 5525.305 kb on - strand at 5525.305 kb on - strand at 5525.305 kb on - strand at 5525.305 kb on - strand at 5525.306 kb on + strand at 5525.307 kb on - strand at 5525.307 kb on - strand at 5525.307 kb on - strand at 5525.307 kb on - strand at 5525.307 kb on - strand at 5525.307 kb on - strand at 5525.310 kb on + strand at 5525.310 kb on + strand at 5525.311 kb on - strand at 5525.311 kb on - strand at 5525.317 kb on + strand at 5525.317 kb on + strand at 5525.317 kb on + strand at 5525.317 kb on + strand at 5525.317 kb on + strand at 5525.317 kb on + strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.318 kb on - strand at 5525.337 kb on + strand at 5525.338 kb on - strand at 5525.432 kb on + strand at 5525.433 kb on - strand at 5525.433 kb on - strand at 5525.433 kb on - strand at 5525.436 kb on + strand at 5525.436 kb on + strand at 5525.436 kb on + strand at 5525.436 kb on + strand at 5525.437 kb on - strand at 5525.462 kb on + strand at 5525.463 kb on - strand at 5525.463 kb on - strand at 5525.463 kb on - strand at 5525.481 kb on - strand at 5525.495 kb on + strand at 5525.496 kb on - strand at 5525.496 kb on - strand at 5525.496 kb on - strand at 5525.496 kb on - strand at 5525.499 kb on - strand at 5525.563 kb on + strand at 5525.565 kb on + strand at 5525.565 kb on + strand at 5525.565 kb on + strand at 5525.565 kb on + strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.566 kb on - strand at 5525.572 kb on + strand at 5525.572 kb on + strand at 5525.573 kb on - strand at 5525.573 kb on - strand at 5525.590 kb on + strand at 5525.590 kb on + strand at 5525.710 kb on + strand, within Pf6N2E2_4782 at 5525.711 kb on - strand, within Pf6N2E2_4782 at 5525.711 kb on - strand, within Pf6N2E2_4782 at 5525.768 kb on - strand, within Pf6N2E2_4782 at 5525.792 kb on - strand, within Pf6N2E2_4782 at 5525.855 kb on + strand, within Pf6N2E2_4782 at 5525.855 kb on + strand, within Pf6N2E2_4782 at 5525.856 kb on - strand, within Pf6N2E2_4782 at 5525.868 kb on + strand, within Pf6N2E2_4782 at 5525.868 kb on + strand, within Pf6N2E2_4782 at 5525.868 kb on + strand, within Pf6N2E2_4782 at 5525.868 kb on + strand, within Pf6N2E2_4782 at 5525.869 kb on - strand, within Pf6N2E2_4782 at 5525.869 kb on - strand, within Pf6N2E2_4782 at 5525.869 kb on - strand, within Pf6N2E2_4782 at 5525.869 kb on - strand, within Pf6N2E2_4782 at 5525.961 kb on + strand, within Pf6N2E2_4782 at 5525.961 kb on + strand, within Pf6N2E2_4782 at 5525.962 kb on - strand, within Pf6N2E2_4782 at 5526.013 kb on + strand, within Pf6N2E2_4782 at 5526.014 kb on - strand, within Pf6N2E2_4782 at 5526.014 kb on - strand, within Pf6N2E2_4782 at 5526.014 kb on - strand, within Pf6N2E2_4782 at 5526.015 kb on + strand, within Pf6N2E2_4782 at 5526.015 kb on + strand, within Pf6N2E2_4782 at 5526.015 kb on + strand, within Pf6N2E2_4782 at 5526.016 kb on - strand, within Pf6N2E2_4782 at 5526.016 kb on - strand, within Pf6N2E2_4782 at 5526.016 kb on - strand, within Pf6N2E2_4782 at 5526.016 kb on - strand, within Pf6N2E2_4782 at 5526.016 kb on - strand, within Pf6N2E2_4782 at 5526.016 kb on - strand, within Pf6N2E2_4782 at 5526.047 kb on - strand, within Pf6N2E2_4782 at 5526.078 kb on + strand, within Pf6N2E2_4782 at 5526.078 kb on + strand, within Pf6N2E2_4782 at 5526.079 kb on - strand, within Pf6N2E2_4782 at 5526.079 kb on - strand, within Pf6N2E2_4782 at 5526.079 kb on - strand, within Pf6N2E2_4782 at 5526.079 kb on - strand, within Pf6N2E2_4782 at 5526.104 kb on - strand, within Pf6N2E2_4782 at 5526.141 kb on + strand, within Pf6N2E2_4782 at 5526.196 kb on + strand, within Pf6N2E2_4782 at 5526.197 kb on - strand, within Pf6N2E2_4782 at 5526.197 kb on - strand, within Pf6N2E2_4782 at 5526.197 kb on - strand, within Pf6N2E2_4782 at 5526.197 kb on - strand, within Pf6N2E2_4782 at 5526.197 kb on - strand, within Pf6N2E2_4782 at 5526.208 kb on + strand, within Pf6N2E2_4782 at 5526.255 kb on + strand, within Pf6N2E2_4782 at 5526.255 kb on + strand, within Pf6N2E2_4782 at 5526.256 kb on - strand, within Pf6N2E2_4782 at 5526.256 kb on - strand, within Pf6N2E2_4782 at 5526.256 kb on - strand, within Pf6N2E2_4782 at 5526.272 kb on - strand, within Pf6N2E2_4782 at 5526.290 kb on + strand, within Pf6N2E2_4782 at 5526.290 kb on + strand, within Pf6N2E2_4782 at 5526.290 kb on + strand, within Pf6N2E2_4782 at 5526.291 kb on - strand, within Pf6N2E2_4782 at 5526.306 kb on + strand, within Pf6N2E2_4782 at 5526.306 kb on + strand, within Pf6N2E2_4782 at 5526.307 kb on - strand, within Pf6N2E2_4782 at 5526.314 kb on + strand, within Pf6N2E2_4782 at 5526.315 kb on - strand, within Pf6N2E2_4782 at 5526.315 kb on - strand, within Pf6N2E2_4782 at 5526.315 kb on - strand, within Pf6N2E2_4782 at 5526.315 kb on - strand, within Pf6N2E2_4782 at 5526.327 kb on + strand, within Pf6N2E2_4782 at 5526.328 kb on - strand, within Pf6N2E2_4782 at 5526.328 kb on - strand, within Pf6N2E2_4782 at 5526.328 kb on - strand, within Pf6N2E2_4782 at 5526.332 kb on - strand, within Pf6N2E2_4782 at 5526.391 kb on - strand, within Pf6N2E2_4782 at 5526.391 kb on - strand, within Pf6N2E2_4782 at 5526.490 kb on + strand, within Pf6N2E2_4782 at 5526.531 kb on + strand, within Pf6N2E2_4782 at 5526.532 kb on - strand, within Pf6N2E2_4782 at 5526.560 kb on - strand, within Pf6N2E2_4782 at 5526.653 kb on + strand, within Pf6N2E2_4782 at 5526.692 kb on - strand, within Pf6N2E2_4782 at 5526.704 kb on + strand, within Pf6N2E2_4782 at 5526.705 kb on - strand, within Pf6N2E2_4782 at 5526.705 kb on - strand, within Pf6N2E2_4782 at 5526.705 kb on - strand, within Pf6N2E2_4782 at 5526.705 kb on - strand, within Pf6N2E2_4782 at 5526.706 kb on + strand, within Pf6N2E2_4782 at 5526.936 kb on + strand, within Pf6N2E2_4782 at 5526.980 kb on + strand at 5526.980 kb on + strand at 5526.981 kb on - strand at 5527.044 kb on + strand at 5527.076 kb on + strand at 5527.076 kb on + strand at 5527.077 kb on - strand at 5527.142 kb on + strand at 5527.142 kb on + strand at 5527.142 kb on + strand at 5528.034 kb on + strand at 5528.034 kb on + strand at 5528.034 kb on + strand at 5528.035 kb on - strand at 5528.048 kb on + strand at 5528.048 kb on + strand at 5528.048 kb on + strand at 5528.048 kb on + strand at 5528.048 kb on + strand at 5528.048 kb on + strand at 5528.049 kb on - strand at 5528.050 kb on + strand at 5528.051 kb on - strand at 5528.061 kb on + strand at 5528.062 kb on - strand at 5528.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,524,670 - -0.4 5,524,802 - +1.4 5,524,802 - -0.5 5,524,814 + -0.0 5,524,814 + -0.2 5,524,815 - +0.6 5,524,815 - +1.0 5,524,817 + -1.4 5,524,817 + -0.2 5,524,817 + -0.0 5,524,817 + +1.4 5,524,817 + +0.3 5,524,818 - +0.3 5,524,818 - +0.4 5,524,818 - -0.8 5,524,818 - +1.4 5,524,898 + +1.2 5,524,898 + +1.1 5,524,899 - +0.4 5,524,899 - +0.0 5,524,899 - -0.3 5,524,950 + -1.1 5,524,950 + -1.0 5,524,951 - -0.4 5,525,165 - -1.7 5,525,165 - +0.3 5,525,165 - -1.9 5,525,193 + +0.8 5,525,193 + -0.2 5,525,194 - -0.9 5,525,194 - +0.4 5,525,194 - +0.1 5,525,194 - +1.4 5,525,194 - +1.0 5,525,194 - -1.9 5,525,209 + -2.1 5,525,209 + -0.1 5,525,210 - +0.3 5,525,221 + -1.6 5,525,237 + -0.5 5,525,238 - +0.2 5,525,238 - -0.3 5,525,266 - +0.3 5,525,302 - +0.7 5,525,302 - -0.3 5,525,302 - -0.8 5,525,304 + +0.4 5,525,305 - -0.3 5,525,305 - -0.4 5,525,305 - -0.3 5,525,305 - +0.0 5,525,306 + +0.2 5,525,307 - +0.6 5,525,307 - -1.6 5,525,307 - +0.7 5,525,307 - +0.7 5,525,307 - -0.2 5,525,307 - +0.4 5,525,310 + -0.7 5,525,310 + -1.8 5,525,311 - +1.7 5,525,311 - -0.1 5,525,317 + +0.5 5,525,317 + -0.4 5,525,317 + -0.7 5,525,317 + -2.3 5,525,317 + +0.6 5,525,317 + +3.5 5,525,318 - -0.1 5,525,318 - +0.5 5,525,318 - +2.2 5,525,318 - +0.4 5,525,318 - -0.7 5,525,318 - +0.4 5,525,318 - -1.9 5,525,318 - +0.7 5,525,318 - +0.5 5,525,318 - -0.9 5,525,318 - -1.8 5,525,318 - -1.8 5,525,337 + -0.6 5,525,338 - +0.5 5,525,432 + +1.4 5,525,433 - +1.5 5,525,433 - +2.6 5,525,433 - -2.0 5,525,436 + -1.5 5,525,436 + +0.8 5,525,436 + +0.2 5,525,436 + -0.1 5,525,437 - +1.1 5,525,462 + +0.2 5,525,463 - +0.1 5,525,463 - +1.5 5,525,463 - -0.3 5,525,481 - -1.7 5,525,495 + -0.2 5,525,496 - +0.4 5,525,496 - -0.8 5,525,496 - -0.0 5,525,496 - -1.0 5,525,499 - -0.1 5,525,563 + +2.2 5,525,565 + +0.0 5,525,565 + -0.3 5,525,565 + +0.2 5,525,565 + -0.7 5,525,566 - +0.3 5,525,566 - +0.3 5,525,566 - -0.4 5,525,566 - -0.4 5,525,566 - +0.7 5,525,566 - +1.8 5,525,566 - -1.9 5,525,566 - -0.2 5,525,566 - +0.5 5,525,566 - -0.8 5,525,566 - +0.0 5,525,566 - -0.6 5,525,572 + -0.4 5,525,572 + -0.4 5,525,573 - -0.3 5,525,573 - +1.1 5,525,590 + +0.1 5,525,590 + +1.1 5,525,710 + Pf6N2E2_4782 0.11 +1.3 5,525,711 - Pf6N2E2_4782 0.11 +1.1 5,525,711 - Pf6N2E2_4782 0.11 +1.5 5,525,768 - Pf6N2E2_4782 0.15 -0.7 5,525,792 - Pf6N2E2_4782 0.16 +1.1 5,525,855 + Pf6N2E2_4782 0.20 -0.7 5,525,855 + Pf6N2E2_4782 0.20 -0.4 5,525,856 - Pf6N2E2_4782 0.20 +0.2 5,525,868 + Pf6N2E2_4782 0.21 +1.0 5,525,868 + Pf6N2E2_4782 0.21 -0.1 5,525,868 + Pf6N2E2_4782 0.21 +1.7 5,525,868 + Pf6N2E2_4782 0.21 +2.9 5,525,869 - Pf6N2E2_4782 0.21 +2.4 5,525,869 - Pf6N2E2_4782 0.21 +1.3 5,525,869 - Pf6N2E2_4782 0.21 +0.3 5,525,869 - Pf6N2E2_4782 0.21 +0.3 5,525,961 + Pf6N2E2_4782 0.27 +0.9 5,525,961 + Pf6N2E2_4782 0.27 -2.2 5,525,962 - Pf6N2E2_4782 0.27 +0.6 5,526,013 + Pf6N2E2_4782 0.30 +0.7 5,526,014 - Pf6N2E2_4782 0.30 -2.8 5,526,014 - Pf6N2E2_4782 0.30 +1.1 5,526,014 - Pf6N2E2_4782 0.30 -0.1 5,526,015 + Pf6N2E2_4782 0.30 +1.4 5,526,015 + Pf6N2E2_4782 0.30 +1.5 5,526,015 + Pf6N2E2_4782 0.30 +0.5 5,526,016 - Pf6N2E2_4782 0.30 +1.4 5,526,016 - Pf6N2E2_4782 0.30 +2.1 5,526,016 - Pf6N2E2_4782 0.30 +2.5 5,526,016 - Pf6N2E2_4782 0.30 +2.1 5,526,016 - Pf6N2E2_4782 0.30 +0.1 5,526,016 - Pf6N2E2_4782 0.30 -0.7 5,526,047 - Pf6N2E2_4782 0.32 +1.0 5,526,078 + Pf6N2E2_4782 0.34 +0.9 5,526,078 + Pf6N2E2_4782 0.34 -0.2 5,526,079 - Pf6N2E2_4782 0.34 +1.0 5,526,079 - Pf6N2E2_4782 0.34 -1.4 5,526,079 - Pf6N2E2_4782 0.34 +0.5 5,526,079 - Pf6N2E2_4782 0.34 -1.1 5,526,104 - Pf6N2E2_4782 0.36 +0.6 5,526,141 + Pf6N2E2_4782 0.38 -1.4 5,526,196 + Pf6N2E2_4782 0.42 +0.2 5,526,197 - Pf6N2E2_4782 0.42 +1.6 5,526,197 - Pf6N2E2_4782 0.42 +0.3 5,526,197 - Pf6N2E2_4782 0.42 +0.7 5,526,197 - Pf6N2E2_4782 0.42 +0.9 5,526,197 - Pf6N2E2_4782 0.42 +2.9 5,526,208 + Pf6N2E2_4782 0.43 -0.5 5,526,255 + Pf6N2E2_4782 0.46 +1.0 5,526,255 + Pf6N2E2_4782 0.46 +0.2 5,526,256 - Pf6N2E2_4782 0.46 -0.0 5,526,256 - Pf6N2E2_4782 0.46 +0.5 5,526,256 - Pf6N2E2_4782 0.46 -1.6 5,526,272 - Pf6N2E2_4782 0.47 +0.7 5,526,290 + Pf6N2E2_4782 0.48 -0.5 5,526,290 + Pf6N2E2_4782 0.48 -1.0 5,526,290 + Pf6N2E2_4782 0.48 +1.1 5,526,291 - Pf6N2E2_4782 0.48 +0.3 5,526,306 + Pf6N2E2_4782 0.49 -1.7 5,526,306 + Pf6N2E2_4782 0.49 +0.3 5,526,307 - Pf6N2E2_4782 0.49 +0.1 5,526,314 + Pf6N2E2_4782 0.49 +0.5 5,526,315 - Pf6N2E2_4782 0.49 +1.9 5,526,315 - Pf6N2E2_4782 0.49 -2.4 5,526,315 - Pf6N2E2_4782 0.49 +1.8 5,526,315 - Pf6N2E2_4782 0.49 -0.2 5,526,327 + Pf6N2E2_4782 0.50 -1.7 5,526,328 - Pf6N2E2_4782 0.50 +2.0 5,526,328 - Pf6N2E2_4782 0.50 +2.4 5,526,328 - Pf6N2E2_4782 0.50 +0.8 5,526,332 - Pf6N2E2_4782 0.51 +2.1 5,526,391 - Pf6N2E2_4782 0.54 +1.0 5,526,391 - Pf6N2E2_4782 0.54 -2.2 5,526,490 + Pf6N2E2_4782 0.61 +0.9 5,526,531 + Pf6N2E2_4782 0.63 +0.3 5,526,532 - Pf6N2E2_4782 0.63 -0.6 5,526,560 - Pf6N2E2_4782 0.65 +0.5 5,526,653 + Pf6N2E2_4782 0.71 +0.8 5,526,692 - Pf6N2E2_4782 0.73 +0.5 5,526,704 + Pf6N2E2_4782 0.74 -0.0 5,526,705 - Pf6N2E2_4782 0.74 -0.3 5,526,705 - Pf6N2E2_4782 0.74 +0.8 5,526,705 - Pf6N2E2_4782 0.74 +0.6 5,526,705 - Pf6N2E2_4782 0.74 +1.4 5,526,706 + Pf6N2E2_4782 0.74 +0.6 5,526,936 + Pf6N2E2_4782 0.89 +0.9 5,526,980 + -0.3 5,526,980 + +0.5 5,526,981 - +1.1 5,527,044 + +1.0 5,527,076 + -0.1 5,527,076 + -0.2 5,527,077 - +0.6 5,527,142 + -0.2 5,527,142 + +1.7 5,527,142 + +0.1 5,528,034 + -1.3 5,528,034 + -1.0 5,528,034 + +0.0 5,528,035 - +0.6 5,528,048 + +1.7 5,528,048 + +0.1 5,528,048 + +0.3 5,528,048 + -2.4 5,528,048 + +1.2 5,528,048 + -0.3 5,528,049 - +1.2 5,528,050 + -0.4 5,528,051 - -0.4 5,528,061 + +0.7 5,528,062 - +0.7 5,528,062 - -0.3
Or see this region's nucleotide sequence