Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4302 and Pf6N2E2_4303 are separated by 11 nucleotides Pf6N2E2_4303 and Pf6N2E2_4304 are separated by 10 nucleotides
Pf6N2E2_4301 - Protein ImpG/VasA, at 5,015,089 to 5,016,876
_4301
Pf6N2E2_4302 - Uncharacterized protein ImpH/VasB, at 5,016,840 to 5,017,844
_4302
Pf6N2E2_4303 - ClpB protein, at 5,017,856 to 5,020,441
_4303
Pf6N2E2_4304 - Sigma-54 dependent transcriptional regulator, at 5,020,452 to 5,021,969
_4304
Position (kb)
5017
5018
5019
5020
5021 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 5016.872 kb on + strand at 5016.872 kb on + strand at 5016.873 kb on - strand at 5016.873 kb on - strand at 5016.873 kb on - strand at 5016.873 kb on - strand at 5016.873 kb on - strand at 5016.873 kb on - strand at 5016.873 kb on - strand at 5016.874 kb on + strand at 5016.910 kb on + strand at 5017.137 kb on + strand, within Pf6N2E2_4302 at 5017.137 kb on + strand, within Pf6N2E2_4302 at 5017.137 kb on + strand, within Pf6N2E2_4302 at 5017.138 kb on - strand, within Pf6N2E2_4302 at 5017.138 kb on - strand, within Pf6N2E2_4302 at 5017.138 kb on - strand, within Pf6N2E2_4302 at 5017.242 kb on + strand, within Pf6N2E2_4302 at 5017.242 kb on + strand, within Pf6N2E2_4302 at 5017.242 kb on + strand, within Pf6N2E2_4302 at 5017.242 kb on + strand, within Pf6N2E2_4302 at 5017.242 kb on + strand, within Pf6N2E2_4302 at 5017.243 kb on - strand, within Pf6N2E2_4302 at 5017.243 kb on - strand, within Pf6N2E2_4302 at 5017.274 kb on + strand, within Pf6N2E2_4302 at 5017.274 kb on + strand, within Pf6N2E2_4302 at 5017.275 kb on - strand, within Pf6N2E2_4302 at 5017.275 kb on - strand, within Pf6N2E2_4302 at 5017.431 kb on + strand, within Pf6N2E2_4302 at 5017.432 kb on - strand, within Pf6N2E2_4302 at 5017.434 kb on + strand, within Pf6N2E2_4302 at 5017.434 kb on + strand, within Pf6N2E2_4302 at 5017.434 kb on + strand, within Pf6N2E2_4302 at 5017.435 kb on - strand, within Pf6N2E2_4302 at 5017.435 kb on - strand, within Pf6N2E2_4302 at 5017.526 kb on + strand, within Pf6N2E2_4302 at 5017.527 kb on - strand, within Pf6N2E2_4302 at 5017.701 kb on + strand, within Pf6N2E2_4302 at 5017.866 kb on + strand at 5017.867 kb on - strand at 5017.867 kb on - strand at 5018.244 kb on - strand, within Pf6N2E2_4303 at 5018.349 kb on + strand, within Pf6N2E2_4303 at 5018.553 kb on + strand, within Pf6N2E2_4303 at 5018.553 kb on + strand, within Pf6N2E2_4303 at 5018.553 kb on + strand, within Pf6N2E2_4303 at 5018.554 kb on - strand, within Pf6N2E2_4303 at 5018.622 kb on + strand, within Pf6N2E2_4303 at 5018.623 kb on - strand, within Pf6N2E2_4303 at 5018.623 kb on - strand, within Pf6N2E2_4303 at 5018.797 kb on + strand, within Pf6N2E2_4303 at 5018.797 kb on + strand, within Pf6N2E2_4303 at 5018.798 kb on - strand, within Pf6N2E2_4303 at 5018.798 kb on - strand, within Pf6N2E2_4303 at 5018.798 kb on - strand, within Pf6N2E2_4303 at 5018.800 kb on + strand, within Pf6N2E2_4303 at 5018.960 kb on + strand, within Pf6N2E2_4303 at 5018.960 kb on + strand, within Pf6N2E2_4303 at 5018.960 kb on + strand, within Pf6N2E2_4303 at 5018.960 kb on + strand, within Pf6N2E2_4303 at 5018.961 kb on - strand, within Pf6N2E2_4303 at 5019.216 kb on + strand, within Pf6N2E2_4303 at 5019.217 kb on - strand, within Pf6N2E2_4303 at 5019.389 kb on + strand, within Pf6N2E2_4303 at 5019.390 kb on - strand, within Pf6N2E2_4303 at 5019.390 kb on - strand, within Pf6N2E2_4303 at 5019.431 kb on - strand, within Pf6N2E2_4303 at 5019.431 kb on - strand, within Pf6N2E2_4303 at 5019.431 kb on - strand, within Pf6N2E2_4303 at 5019.557 kb on - strand, within Pf6N2E2_4303 at 5019.717 kb on - strand, within Pf6N2E2_4303 at 5019.772 kb on + strand, within Pf6N2E2_4303 at 5019.773 kb on - strand, within Pf6N2E2_4303 at 5019.773 kb on - strand, within Pf6N2E2_4303 at 5019.806 kb on + strand, within Pf6N2E2_4303 at 5019.806 kb on + strand, within Pf6N2E2_4303 at 5020.089 kb on + strand, within Pf6N2E2_4303 at 5020.090 kb on - strand, within Pf6N2E2_4303 at 5020.142 kb on + strand, within Pf6N2E2_4303 at 5020.146 kb on - strand, within Pf6N2E2_4303 at 5020.167 kb on + strand, within Pf6N2E2_4303 at 5020.168 kb on - strand, within Pf6N2E2_4303 at 5020.168 kb on - strand, within Pf6N2E2_4303 at 5020.238 kb on + strand at 5020.239 kb on - strand at 5020.282 kb on + strand at 5020.282 kb on + strand at 5020.282 kb on + strand at 5020.392 kb on + strand at 5020.392 kb on + strand at 5020.392 kb on + strand at 5020.392 kb on + strand at 5020.393 kb on - strand at 5020.393 kb on - strand at 5020.393 kb on - strand at 5020.482 kb on + strand at 5020.482 kb on + strand at 5020.483 kb on - strand at 5020.483 kb on - strand at 5020.602 kb on + strand at 5020.602 kb on + strand at 5020.602 kb on + strand at 5020.602 kb on + strand at 5020.699 kb on - strand, within Pf6N2E2_4304 at 5020.762 kb on + strand, within Pf6N2E2_4304 at 5020.873 kb on + strand, within Pf6N2E2_4304 at 5020.874 kb on - strand, within Pf6N2E2_4304 at 5020.963 kb on + strand, within Pf6N2E2_4304 at 5020.963 kb on + strand, within Pf6N2E2_4304 at 5020.964 kb on - strand, within Pf6N2E2_4304 at 5020.997 kb on - strand, within Pf6N2E2_4304 at 5021.004 kb on - strand, within Pf6N2E2_4304 at 5021.031 kb on + strand, within Pf6N2E2_4304 at 5021.032 kb on - strand, within Pf6N2E2_4304 at 5021.045 kb on + strand, within Pf6N2E2_4304 at 5021.046 kb on - strand, within Pf6N2E2_4304 at 5021.085 kb on - strand, within Pf6N2E2_4304 at 5021.104 kb on - strand, within Pf6N2E2_4304 at 5021.195 kb on + strand, within Pf6N2E2_4304 at 5021.195 kb on + strand, within Pf6N2E2_4304
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,016,872 + -1.2 5,016,872 + -0.4 5,016,873 - +1.8 5,016,873 - +0.7 5,016,873 - +0.1 5,016,873 - -0.9 5,016,873 - -0.5 5,016,873 - -0.5 5,016,873 - +0.8 5,016,874 + -0.2 5,016,910 + +0.1 5,017,137 + Pf6N2E2_4302 0.30 -0.9 5,017,137 + Pf6N2E2_4302 0.30 +0.4 5,017,137 + Pf6N2E2_4302 0.30 +0.6 5,017,138 - Pf6N2E2_4302 0.30 +0.7 5,017,138 - Pf6N2E2_4302 0.30 +0.3 5,017,138 - Pf6N2E2_4302 0.30 -0.4 5,017,242 + Pf6N2E2_4302 0.40 +1.1 5,017,242 + Pf6N2E2_4302 0.40 +0.7 5,017,242 + Pf6N2E2_4302 0.40 -0.9 5,017,242 + Pf6N2E2_4302 0.40 -1.9 5,017,242 + Pf6N2E2_4302 0.40 +0.9 5,017,243 - Pf6N2E2_4302 0.40 +2.4 5,017,243 - Pf6N2E2_4302 0.40 -3.0 5,017,274 + Pf6N2E2_4302 0.43 -0.8 5,017,274 + Pf6N2E2_4302 0.43 +0.5 5,017,275 - Pf6N2E2_4302 0.43 +0.1 5,017,275 - Pf6N2E2_4302 0.43 +0.7 5,017,431 + Pf6N2E2_4302 0.59 +1.2 5,017,432 - Pf6N2E2_4302 0.59 -1.6 5,017,434 + Pf6N2E2_4302 0.59 -0.8 5,017,434 + Pf6N2E2_4302 0.59 -1.9 5,017,434 + Pf6N2E2_4302 0.59 +0.8 5,017,435 - Pf6N2E2_4302 0.59 -0.4 5,017,435 - Pf6N2E2_4302 0.59 +0.3 5,017,526 + Pf6N2E2_4302 0.68 -0.7 5,017,527 - Pf6N2E2_4302 0.68 +0.3 5,017,701 + Pf6N2E2_4302 0.86 +0.2 5,017,866 + -1.2 5,017,867 - +2.2 5,017,867 - -0.1 5,018,244 - Pf6N2E2_4303 0.15 -2.3 5,018,349 + Pf6N2E2_4303 0.19 -0.6 5,018,553 + Pf6N2E2_4303 0.27 +0.6 5,018,553 + Pf6N2E2_4303 0.27 -1.9 5,018,553 + Pf6N2E2_4303 0.27 -0.1 5,018,554 - Pf6N2E2_4303 0.27 +1.8 5,018,622 + Pf6N2E2_4303 0.30 +0.2 5,018,623 - Pf6N2E2_4303 0.30 -0.8 5,018,623 - Pf6N2E2_4303 0.30 -1.7 5,018,797 + Pf6N2E2_4303 0.36 +2.3 5,018,797 + Pf6N2E2_4303 0.36 -1.8 5,018,798 - Pf6N2E2_4303 0.36 +0.1 5,018,798 - Pf6N2E2_4303 0.36 -0.1 5,018,798 - Pf6N2E2_4303 0.36 +0.1 5,018,800 + Pf6N2E2_4303 0.37 -0.7 5,018,960 + Pf6N2E2_4303 0.43 +1.1 5,018,960 + Pf6N2E2_4303 0.43 -0.5 5,018,960 + Pf6N2E2_4303 0.43 +0.1 5,018,960 + Pf6N2E2_4303 0.43 -0.1 5,018,961 - Pf6N2E2_4303 0.43 -0.0 5,019,216 + Pf6N2E2_4303 0.53 +0.5 5,019,217 - Pf6N2E2_4303 0.53 -1.5 5,019,389 + Pf6N2E2_4303 0.59 -0.0 5,019,390 - Pf6N2E2_4303 0.59 +0.3 5,019,390 - Pf6N2E2_4303 0.59 -0.6 5,019,431 - Pf6N2E2_4303 0.61 -3.3 5,019,431 - Pf6N2E2_4303 0.61 -0.2 5,019,431 - Pf6N2E2_4303 0.61 +4.3 5,019,557 - Pf6N2E2_4303 0.66 +0.5 5,019,717 - Pf6N2E2_4303 0.72 -2.2 5,019,772 + Pf6N2E2_4303 0.74 +0.3 5,019,773 - Pf6N2E2_4303 0.74 -0.2 5,019,773 - Pf6N2E2_4303 0.74 +0.4 5,019,806 + Pf6N2E2_4303 0.75 +0.7 5,019,806 + Pf6N2E2_4303 0.75 +0.6 5,020,089 + Pf6N2E2_4303 0.86 -0.8 5,020,090 - Pf6N2E2_4303 0.86 -0.8 5,020,142 + Pf6N2E2_4303 0.88 -0.5 5,020,146 - Pf6N2E2_4303 0.89 +1.1 5,020,167 + Pf6N2E2_4303 0.89 -0.0 5,020,168 - Pf6N2E2_4303 0.89 -0.1 5,020,168 - Pf6N2E2_4303 0.89 +1.2 5,020,238 + -0.2 5,020,239 - -1.8 5,020,282 + +0.5 5,020,282 + -0.1 5,020,282 + -0.5 5,020,392 + -0.1 5,020,392 + -0.7 5,020,392 + -1.6 5,020,392 + -0.6 5,020,393 - -1.2 5,020,393 - -0.9 5,020,393 - +0.2 5,020,482 + +0.6 5,020,482 + +0.6 5,020,483 - -0.7 5,020,483 - -2.0 5,020,602 + -0.1 5,020,602 + +1.5 5,020,602 + -0.4 5,020,602 + -1.8 5,020,699 - Pf6N2E2_4304 0.16 -0.6 5,020,762 + Pf6N2E2_4304 0.20 +0.6 5,020,873 + Pf6N2E2_4304 0.28 +0.3 5,020,874 - Pf6N2E2_4304 0.28 +0.8 5,020,963 + Pf6N2E2_4304 0.34 +1.0 5,020,963 + Pf6N2E2_4304 0.34 +1.1 5,020,964 - Pf6N2E2_4304 0.34 -0.2 5,020,997 - Pf6N2E2_4304 0.36 -2.6 5,021,004 - Pf6N2E2_4304 0.36 -0.0 5,021,031 + Pf6N2E2_4304 0.38 -1.7 5,021,032 - Pf6N2E2_4304 0.38 +2.4 5,021,045 + Pf6N2E2_4304 0.39 +0.6 5,021,046 - Pf6N2E2_4304 0.39 -0.7 5,021,085 - Pf6N2E2_4304 0.42 +1.9 5,021,104 - Pf6N2E2_4304 0.43 +0.0 5,021,195 + Pf6N2E2_4304 0.49 -0.1 5,021,195 + Pf6N2E2_4304 0.49 +1.5
Or see this region's nucleotide sequence