Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3769

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3767 and Pf6N2E2_3768 are separated by 1 nucleotidesPf6N2E2_3768 and Pf6N2E2_3769 are separated by 267 nucleotidesPf6N2E2_3769 and Pf6N2E2_3770 are separated by 56 nucleotides Pf6N2E2_3767 - Arginyl-tRNA synthetase (EC 6.1.1.19), at 4,389,665 to 4,391,401 _3767 Pf6N2E2_3768 - Cell division protein FtsN, at 4,391,403 to 4,392,098 _3768 Pf6N2E2_3769 - ATP-dependent protease HslV (EC 3.4.25.-), at 4,392,366 to 4,392,896 _3769 Pf6N2E2_3770 - ATP-dependent hsl protease ATP-binding subunit HslU, at 4,392,953 to 4,394,290 _3770 Position (kb) 4392 4393Strain fitness (log2 ratio) -1 0 1at 4392.076 kb on + strandat 4392.076 kb on + strandat 4392.319 kb on - strandat 4392.950 kb on - strandat 4392.952 kb on + strandat 4392.953 kb on - strandat 4392.953 kb on - strandat 4392.953 kb on - strandat 4392.953 kb on - strandat 4393.501 kb on + strand, within Pf6N2E2_3770at 4393.849 kb on + strand, within Pf6N2E2_3770at 4393.849 kb on + strand, within Pf6N2E2_3770at 4393.849 kb on + strand, within Pf6N2E2_3770at 4393.850 kb on - strand, within Pf6N2E2_3770

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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4,392,076 + -1.2
4,392,076 + -1.5
4,392,319 - -1.2
4,392,950 - +0.0
4,392,952 + -0.5
4,392,953 - +0.8
4,392,953 - -0.7
4,392,953 - +1.5
4,392,953 - +1.5
4,393,501 + Pf6N2E2_3770 0.41 -1.4
4,393,849 + Pf6N2E2_3770 0.67 +0.6
4,393,849 + Pf6N2E2_3770 0.67 -1.0
4,393,849 + Pf6N2E2_3770 0.67 -0.3
4,393,850 - Pf6N2E2_3770 0.67 -0.2

Or see this region's nucleotide sequence