Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3505 and Pf6N2E2_3506 are separated by 168 nucleotides Pf6N2E2_3506 and Pf6N2E2_3507 are separated by 36 nucleotides Pf6N2E2_3507 and Pf6N2E2_3508 are separated by 284 nucleotides
Pf6N2E2_3505 - Translation initiation factor SUI1-related protein, at 4,086,011 to 4,086,382
_3505
Pf6N2E2_3506 - Putative Nudix hydrolase YfcD (EC 3.6.-.-), at 4,086,551 to 4,087,075
_3506
Pf6N2E2_3507 - FIG014328: hypothetical protein, at 4,087,112 to 4,088,179
_3507
Pf6N2E2_3508 - Probable two-component response regulator, at 4,088,464 to 4,090,134
_3508
Position (kb)
4087
4088
4089 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4086.338 kb on + strand, within Pf6N2E2_3505 at 4086.338 kb on + strand, within Pf6N2E2_3505 at 4086.552 kb on + strand at 4086.552 kb on + strand at 4086.553 kb on - strand at 4086.553 kb on - strand at 4086.554 kb on + strand at 4086.555 kb on - strand at 4086.555 kb on - strand at 4086.591 kb on + strand at 4086.591 kb on + strand at 4086.592 kb on - strand at 4086.624 kb on + strand, within Pf6N2E2_3506 at 4086.624 kb on + strand, within Pf6N2E2_3506 at 4086.625 kb on - strand, within Pf6N2E2_3506 at 4086.768 kb on + strand, within Pf6N2E2_3506 at 4086.846 kb on + strand, within Pf6N2E2_3506 at 4086.847 kb on - strand, within Pf6N2E2_3506 at 4086.900 kb on + strand, within Pf6N2E2_3506 at 4086.900 kb on + strand, within Pf6N2E2_3506 at 4086.901 kb on - strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.966 kb on + strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4086.967 kb on - strand, within Pf6N2E2_3506 at 4087.170 kb on + strand at 4087.170 kb on + strand at 4087.202 kb on + strand at 4087.203 kb on - strand at 4087.383 kb on + strand, within Pf6N2E2_3507 at 4087.383 kb on + strand, within Pf6N2E2_3507 at 4087.383 kb on + strand, within Pf6N2E2_3507 at 4087.383 kb on + strand, within Pf6N2E2_3507 at 4087.384 kb on - strand, within Pf6N2E2_3507 at 4087.384 kb on - strand, within Pf6N2E2_3507 at 4087.384 kb on - strand, within Pf6N2E2_3507 at 4087.585 kb on - strand, within Pf6N2E2_3507 at 4087.682 kb on + strand, within Pf6N2E2_3507 at 4087.879 kb on - strand, within Pf6N2E2_3507 at 4087.939 kb on - strand, within Pf6N2E2_3507 at 4088.055 kb on + strand, within Pf6N2E2_3507 at 4088.056 kb on - strand, within Pf6N2E2_3507 at 4088.106 kb on + strand at 4088.107 kb on - strand at 4088.226 kb on + strand at 4088.303 kb on + strand at 4088.303 kb on + strand at 4088.303 kb on + strand at 4088.303 kb on + strand at 4088.303 kb on + strand at 4088.303 kb on + strand at 4088.303 kb on + strand at 4088.304 kb on - strand at 4088.304 kb on - strand at 4088.304 kb on - strand at 4088.304 kb on - strand at 4088.304 kb on - strand at 4088.304 kb on - strand at 4088.304 kb on - strand at 4088.305 kb on + strand at 4088.305 kb on + strand at 4088.306 kb on - strand at 4088.306 kb on - strand at 4088.306 kb on - strand at 4088.306 kb on - strand at 4088.329 kb on - strand at 4088.381 kb on + strand at 4088.381 kb on + strand at 4088.381 kb on + strand at 4088.382 kb on - strand at 4088.447 kb on + strand at 4088.448 kb on - strand at 4088.448 kb on - strand at 4088.451 kb on + strand at 4088.451 kb on + strand at 4088.451 kb on + strand at 4088.451 kb on + strand at 4088.451 kb on + strand at 4088.452 kb on - strand at 4088.452 kb on - strand at 4088.455 kb on - strand at 4088.463 kb on + strand at 4088.464 kb on - strand at 4088.523 kb on - strand at 4088.523 kb on - strand at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.839 kb on + strand, within Pf6N2E2_3508 at 4088.840 kb on - strand, within Pf6N2E2_3508 at 4088.840 kb on - strand, within Pf6N2E2_3508 at 4088.840 kb on - strand, within Pf6N2E2_3508 at 4088.840 kb on - strand, within Pf6N2E2_3508 at 4088.840 kb on - strand, within Pf6N2E2_3508
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,086,338 + Pf6N2E2_3505 0.88 +0.4 4,086,338 + Pf6N2E2_3505 0.88 +0.7 4,086,552 + +1.3 4,086,552 + +1.8 4,086,553 - -1.3 4,086,553 - -1.1 4,086,554 + +1.1 4,086,555 - +1.7 4,086,555 - +0.8 4,086,591 + -0.1 4,086,591 + -1.1 4,086,592 - -1.3 4,086,624 + Pf6N2E2_3506 0.14 +0.6 4,086,624 + Pf6N2E2_3506 0.14 +2.1 4,086,625 - Pf6N2E2_3506 0.14 -1.4 4,086,768 + Pf6N2E2_3506 0.41 -3.1 4,086,846 + Pf6N2E2_3506 0.56 -0.2 4,086,847 - Pf6N2E2_3506 0.56 +0.1 4,086,900 + Pf6N2E2_3506 0.66 +0.8 4,086,900 + Pf6N2E2_3506 0.66 +0.3 4,086,901 - Pf6N2E2_3506 0.67 +0.9 4,086,966 + Pf6N2E2_3506 0.79 +0.6 4,086,966 + Pf6N2E2_3506 0.79 +0.2 4,086,966 + Pf6N2E2_3506 0.79 +0.2 4,086,966 + Pf6N2E2_3506 0.79 -0.7 4,086,966 + Pf6N2E2_3506 0.79 -0.8 4,086,967 - Pf6N2E2_3506 0.79 +2.0 4,086,967 - Pf6N2E2_3506 0.79 -1.3 4,086,967 - Pf6N2E2_3506 0.79 +1.6 4,086,967 - Pf6N2E2_3506 0.79 +0.9 4,086,967 - Pf6N2E2_3506 0.79 +0.6 4,086,967 - Pf6N2E2_3506 0.79 -1.2 4,086,967 - Pf6N2E2_3506 0.79 -0.6 4,086,967 - Pf6N2E2_3506 0.79 -0.5 4,086,967 - Pf6N2E2_3506 0.79 +2.5 4,087,170 + +0.9 4,087,170 + +0.6 4,087,202 + +0.2 4,087,203 - -0.9 4,087,383 + Pf6N2E2_3507 0.25 -0.4 4,087,383 + Pf6N2E2_3507 0.25 -1.9 4,087,383 + Pf6N2E2_3507 0.25 -0.6 4,087,383 + Pf6N2E2_3507 0.25 -2.3 4,087,384 - Pf6N2E2_3507 0.25 -0.3 4,087,384 - Pf6N2E2_3507 0.25 +1.5 4,087,384 - Pf6N2E2_3507 0.25 -1.3 4,087,585 - Pf6N2E2_3507 0.44 -0.4 4,087,682 + Pf6N2E2_3507 0.53 -0.0 4,087,879 - Pf6N2E2_3507 0.72 -0.5 4,087,939 - Pf6N2E2_3507 0.77 +0.3 4,088,055 + Pf6N2E2_3507 0.88 -2.4 4,088,056 - Pf6N2E2_3507 0.88 -0.1 4,088,106 + -2.8 4,088,107 - +0.9 4,088,226 + -2.7 4,088,303 + -2.3 4,088,303 + -1.7 4,088,303 + -1.4 4,088,303 + +0.1 4,088,303 + -2.3 4,088,303 + -3.3 4,088,303 + -3.0 4,088,304 - +2.7 4,088,304 - -0.7 4,088,304 - +3.8 4,088,304 - +1.9 4,088,304 - +2.7 4,088,304 - +1.9 4,088,304 - +2.8 4,088,305 + -1.2 4,088,305 + -1.2 4,088,306 - -0.7 4,088,306 - +0.8 4,088,306 - -0.0 4,088,306 - +1.4 4,088,329 - +1.8 4,088,381 + +1.1 4,088,381 + +0.4 4,088,381 + +0.4 4,088,382 - +1.0 4,088,447 + +0.1 4,088,448 - +3.1 4,088,448 - +3.0 4,088,451 + +1.8 4,088,451 + -1.4 4,088,451 + +1.8 4,088,451 + +0.2 4,088,451 + -1.2 4,088,452 - +4.0 4,088,452 - +2.5 4,088,455 - +3.1 4,088,463 + +2.8 4,088,464 - +3.3 4,088,523 - +4.2 4,088,523 - -0.3 4,088,839 + Pf6N2E2_3508 0.22 +1.2 4,088,839 + Pf6N2E2_3508 0.22 +2.6 4,088,839 + Pf6N2E2_3508 0.22 +3.8 4,088,839 + Pf6N2E2_3508 0.22 +3.9 4,088,839 + Pf6N2E2_3508 0.22 +2.5 4,088,839 + Pf6N2E2_3508 0.22 +2.1 4,088,839 + Pf6N2E2_3508 0.22 +2.3 4,088,839 + Pf6N2E2_3508 0.22 +2.6 4,088,839 + Pf6N2E2_3508 0.22 +2.9 4,088,839 + Pf6N2E2_3508 0.22 +1.5 4,088,839 + Pf6N2E2_3508 0.22 +3.2 4,088,839 + Pf6N2E2_3508 0.22 +3.0 4,088,839 + Pf6N2E2_3508 0.22 +3.1 4,088,840 - Pf6N2E2_3508 0.23 +3.8 4,088,840 - Pf6N2E2_3508 0.23 +2.5 4,088,840 - Pf6N2E2_3508 0.23 +2.5 4,088,840 - Pf6N2E2_3508 0.23 +1.9 4,088,840 - Pf6N2E2_3508 0.23 +2.4
Or see this region's nucleotide sequence