Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_322 and Pf6N2E2_323 are separated by 166 nucleotides Pf6N2E2_323 and Pf6N2E2_324 overlap by 4 nucleotides
Pf6N2E2_322 - Transcriptional regulator, LacI family, at 390,422 to 391,420
_322
Pf6N2E2_323 - Nucleoside-diphosphate-sugar epimerases, at 391,587 to 392,762
_323
Pf6N2E2_324 - Inosose isomerase (EC 5.3.99.-), at 392,759 to 393,850
_324
Position (kb) 391
392
393 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 390.626 kb on - strand, within Pf6N2E2_322 at 391.086 kb on + strand, within Pf6N2E2_322 at 391.086 kb on + strand, within Pf6N2E2_322 at 391.207 kb on + strand, within Pf6N2E2_322 at 391.207 kb on + strand, within Pf6N2E2_322 at 391.207 kb on + strand, within Pf6N2E2_322 at 391.208 kb on - strand, within Pf6N2E2_322 at 391.482 kb on + strand at 391.609 kb on + strand at 391.610 kb on - strand at 391.610 kb on - strand at 391.776 kb on + strand, within Pf6N2E2_323 at 391.993 kb on - strand, within Pf6N2E2_323 at 392.006 kb on + strand, within Pf6N2E2_323 at 392.010 kb on + strand, within Pf6N2E2_323 at 392.010 kb on + strand, within Pf6N2E2_323 at 392.010 kb on + strand, within Pf6N2E2_323 at 392.010 kb on + strand, within Pf6N2E2_323 at 392.011 kb on - strand, within Pf6N2E2_323 at 392.138 kb on + strand, within Pf6N2E2_323 at 392.246 kb on + strand, within Pf6N2E2_323 at 392.257 kb on + strand, within Pf6N2E2_323 at 392.257 kb on + strand, within Pf6N2E2_323 at 392.257 kb on + strand, within Pf6N2E2_323 at 392.258 kb on - strand, within Pf6N2E2_323 at 392.258 kb on - strand, within Pf6N2E2_323 at 392.258 kb on - strand, within Pf6N2E2_323 at 392.258 kb on - strand, within Pf6N2E2_323 at 392.404 kb on - strand, within Pf6N2E2_323 at 392.613 kb on - strand, within Pf6N2E2_323 at 392.764 kb on + strand at 392.803 kb on + strand at 392.885 kb on + strand, within Pf6N2E2_324 at 392.886 kb on - strand, within Pf6N2E2_324 at 392.921 kb on + strand, within Pf6N2E2_324 at 392.922 kb on - strand, within Pf6N2E2_324 at 392.922 kb on - strand, within Pf6N2E2_324 at 392.922 kb on - strand, within Pf6N2E2_324 at 392.922 kb on - strand, within Pf6N2E2_324 at 392.922 kb on - strand, within Pf6N2E2_324 at 392.957 kb on + strand, within Pf6N2E2_324 at 392.958 kb on - strand, within Pf6N2E2_324 at 393.215 kb on + strand, within Pf6N2E2_324 at 393.215 kb on + strand, within Pf6N2E2_324 at 393.216 kb on - strand, within Pf6N2E2_324 at 393.332 kb on + strand, within Pf6N2E2_324 at 393.332 kb on + strand, within Pf6N2E2_324 at 393.425 kb on + strand, within Pf6N2E2_324 at 393.425 kb on + strand, within Pf6N2E2_324 at 393.426 kb on - strand, within Pf6N2E2_324 at 393.461 kb on + strand, within Pf6N2E2_324 at 393.461 kb on + strand, within Pf6N2E2_324 at 393.462 kb on - strand, within Pf6N2E2_324 at 393.462 kb on - strand, within Pf6N2E2_324 at 393.462 kb on - strand, within Pf6N2E2_324
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 390,626 - Pf6N2E2_322 0.20 +0.9 391,086 + Pf6N2E2_322 0.66 +0.3 391,086 + Pf6N2E2_322 0.66 -0.6 391,207 + Pf6N2E2_322 0.79 +0.1 391,207 + Pf6N2E2_322 0.79 +0.3 391,207 + Pf6N2E2_322 0.79 -0.3 391,208 - Pf6N2E2_322 0.79 +0.6 391,482 + +2.5 391,609 + -1.0 391,610 - +0.4 391,610 - -0.9 391,776 + Pf6N2E2_323 0.16 -1.7 391,993 - Pf6N2E2_323 0.35 -0.4 392,006 + Pf6N2E2_323 0.36 -1.0 392,010 + Pf6N2E2_323 0.36 +0.3 392,010 + Pf6N2E2_323 0.36 -0.8 392,010 + Pf6N2E2_323 0.36 +0.8 392,010 + Pf6N2E2_323 0.36 -0.3 392,011 - Pf6N2E2_323 0.36 -0.5 392,138 + Pf6N2E2_323 0.47 +0.2 392,246 + Pf6N2E2_323 0.56 -0.0 392,257 + Pf6N2E2_323 0.57 -2.2 392,257 + Pf6N2E2_323 0.57 +0.2 392,257 + Pf6N2E2_323 0.57 -3.7 392,258 - Pf6N2E2_323 0.57 -0.9 392,258 - Pf6N2E2_323 0.57 +1.4 392,258 - Pf6N2E2_323 0.57 +0.5 392,258 - Pf6N2E2_323 0.57 +0.1 392,404 - Pf6N2E2_323 0.69 -0.7 392,613 - Pf6N2E2_323 0.87 -0.6 392,764 + -0.6 392,803 + +0.4 392,885 + Pf6N2E2_324 0.12 -0.3 392,886 - Pf6N2E2_324 0.12 -0.1 392,921 + Pf6N2E2_324 0.15 -0.6 392,922 - Pf6N2E2_324 0.15 -1.1 392,922 - Pf6N2E2_324 0.15 -0.2 392,922 - Pf6N2E2_324 0.15 +0.7 392,922 - Pf6N2E2_324 0.15 -1.3 392,922 - Pf6N2E2_324 0.15 +0.1 392,957 + Pf6N2E2_324 0.18 +1.3 392,958 - Pf6N2E2_324 0.18 +0.3 393,215 + Pf6N2E2_324 0.42 +0.1 393,215 + Pf6N2E2_324 0.42 +0.6 393,216 - Pf6N2E2_324 0.42 +0.7 393,332 + Pf6N2E2_324 0.52 +2.1 393,332 + Pf6N2E2_324 0.52 +0.1 393,425 + Pf6N2E2_324 0.61 +0.4 393,425 + Pf6N2E2_324 0.61 +0.4 393,426 - Pf6N2E2_324 0.61 -0.4 393,461 + Pf6N2E2_324 0.64 -2.5 393,461 + Pf6N2E2_324 0.64 +1.6 393,462 - Pf6N2E2_324 0.64 +0.6 393,462 - Pf6N2E2_324 0.64 -1.3 393,462 - Pf6N2E2_324 0.64 -0.2
Or see this region's nucleotide sequence