Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_321

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_320 and Pf6N2E2_321 are separated by 81 nucleotidesPf6N2E2_321 and Pf6N2E2_322 are separated by 234 nucleotides Pf6N2E2_320 - TRAP transporter, 4TM/12TM fusion protein, unknown substrate 1, at 387,128 to 389,155 _320 Pf6N2E2_321 - TRAP transporter solute receptor, unknown substrate 1, at 389,237 to 390,187 _321 Pf6N2E2_322 - Transcriptional regulator, LacI family, at 390,422 to 391,420 _322 Position (kb) 389 390 391Strain fitness (log2 ratio) -2 -1 0 1 2 3at 388.613 kb on - strand, within Pf6N2E2_320at 388.613 kb on - strand, within Pf6N2E2_320at 388.613 kb on - strand, within Pf6N2E2_320at 388.613 kb on - strand, within Pf6N2E2_320at 388.614 kb on + strand, within Pf6N2E2_320at 388.615 kb on - strand, within Pf6N2E2_320at 388.615 kb on - strand, within Pf6N2E2_320at 388.629 kb on + strand, within Pf6N2E2_320at 388.629 kb on + strand, within Pf6N2E2_320at 388.630 kb on - strand, within Pf6N2E2_320at 388.644 kb on + strand, within Pf6N2E2_320at 388.726 kb on - strand, within Pf6N2E2_320at 388.743 kb on + strand, within Pf6N2E2_320at 388.899 kb on + strand, within Pf6N2E2_320at 389.015 kb on + strandat 389.015 kb on + strandat 389.015 kb on + strandat 389.015 kb on + strandat 389.016 kb on - strandat 389.016 kb on - strandat 389.016 kb on - strandat 389.238 kb on + strandat 389.238 kb on + strandat 389.239 kb on - strandat 389.259 kb on + strandat 389.259 kb on + strandat 389.260 kb on - strandat 389.260 kb on - strandat 389.475 kb on + strand, within Pf6N2E2_321at 389.475 kb on + strand, within Pf6N2E2_321at 389.475 kb on + strand, within Pf6N2E2_321at 389.475 kb on + strand, within Pf6N2E2_321at 389.476 kb on - strand, within Pf6N2E2_321at 389.476 kb on - strand, within Pf6N2E2_321at 389.476 kb on - strand, within Pf6N2E2_321at 389.479 kb on + strand, within Pf6N2E2_321at 389.480 kb on - strand, within Pf6N2E2_321at 389.549 kb on + strand, within Pf6N2E2_321at 389.549 kb on + strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.554 kb on - strand, within Pf6N2E2_321at 389.554 kb on - strand, within Pf6N2E2_321at 389.554 kb on - strand, within Pf6N2E2_321at 389.673 kb on + strand, within Pf6N2E2_321at 389.673 kb on + strand, within Pf6N2E2_321at 389.694 kb on + strand, within Pf6N2E2_321at 389.695 kb on - strand, within Pf6N2E2_321at 389.832 kb on + strand, within Pf6N2E2_321at 389.832 kb on + strand, within Pf6N2E2_321at 390.036 kb on + strand, within Pf6N2E2_321at 390.036 kb on + strand, within Pf6N2E2_321at 390.037 kb on - strand, within Pf6N2E2_321at 390.037 kb on - strand, within Pf6N2E2_321at 390.105 kb on + strandat 390.105 kb on + strandat 390.106 kb on - strandat 390.106 kb on - strandat 390.208 kb on - strandat 390.214 kb on + strandat 390.214 kb on + strandat 390.215 kb on - strandat 390.215 kb on - strandat 390.215 kb on - strandat 390.234 kb on + strandat 390.234 kb on + strandat 390.235 kb on - strandat 390.235 kb on - strandat 390.235 kb on - strandat 390.235 kb on - strandat 390.235 kb on - strandat 390.423 kb on + strandat 390.423 kb on + strandat 390.423 kb on + strandat 390.440 kb on - strandat 390.626 kb on - strand, within Pf6N2E2_322at 391.086 kb on + strand, within Pf6N2E2_322at 391.086 kb on + strand, within Pf6N2E2_322

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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388,613 - Pf6N2E2_320 0.73 +0.8
388,613 - Pf6N2E2_320 0.73 +1.2
388,613 - Pf6N2E2_320 0.73 +0.4
388,613 - Pf6N2E2_320 0.73 +1.1
388,614 + Pf6N2E2_320 0.73 -0.3
388,615 - Pf6N2E2_320 0.73 +0.9
388,615 - Pf6N2E2_320 0.73 +0.0
388,629 + Pf6N2E2_320 0.74 -0.5
388,629 + Pf6N2E2_320 0.74 -1.3
388,630 - Pf6N2E2_320 0.74 -1.8
388,644 + Pf6N2E2_320 0.75 +1.6
388,726 - Pf6N2E2_320 0.79 -0.4
388,743 + Pf6N2E2_320 0.80 +0.5
388,899 + Pf6N2E2_320 0.87 -1.8
389,015 + +0.5
389,015 + +1.2
389,015 + +2.5
389,015 + -0.1
389,016 - -0.0
389,016 - -0.4
389,016 - -0.3
389,238 + -0.5
389,238 + -1.2
389,239 - +0.0
389,259 + -0.3
389,259 + +1.4
389,260 - -0.8
389,260 - -0.3
389,475 + Pf6N2E2_321 0.25 -2.2
389,475 + Pf6N2E2_321 0.25 -0.0
389,475 + Pf6N2E2_321 0.25 -0.2
389,475 + Pf6N2E2_321 0.25 -1.6
389,476 - Pf6N2E2_321 0.25 -1.8
389,476 - Pf6N2E2_321 0.25 +0.6
389,476 - Pf6N2E2_321 0.25 -0.5
389,479 + Pf6N2E2_321 0.25 -0.3
389,480 - Pf6N2E2_321 0.26 +1.1
389,549 + Pf6N2E2_321 0.33 +0.8
389,549 + Pf6N2E2_321 0.33 +0.6
389,550 - Pf6N2E2_321 0.33 +1.6
389,550 - Pf6N2E2_321 0.33 -0.6
389,550 - Pf6N2E2_321 0.33 +0.4
389,550 - Pf6N2E2_321 0.33 -0.5
389,550 - Pf6N2E2_321 0.33 -1.0
389,550 - Pf6N2E2_321 0.33 +2.2
389,553 + Pf6N2E2_321 0.33 -1.2
389,553 + Pf6N2E2_321 0.33 +0.2
389,553 + Pf6N2E2_321 0.33 +0.4
389,553 + Pf6N2E2_321 0.33 +0.4
389,553 + Pf6N2E2_321 0.33 +0.8
389,554 - Pf6N2E2_321 0.33 -0.3
389,554 - Pf6N2E2_321 0.33 -0.5
389,554 - Pf6N2E2_321 0.33 -0.4
389,673 + Pf6N2E2_321 0.46 +1.1
389,673 + Pf6N2E2_321 0.46 +0.0
389,694 + Pf6N2E2_321 0.48 -1.1
389,695 - Pf6N2E2_321 0.48 -0.4
389,832 + Pf6N2E2_321 0.63 -1.4
389,832 + Pf6N2E2_321 0.63 -0.1
390,036 + Pf6N2E2_321 0.84 +0.9
390,036 + Pf6N2E2_321 0.84 -0.5
390,037 - Pf6N2E2_321 0.84 -1.2
390,037 - Pf6N2E2_321 0.84 +1.5
390,105 + +3.6
390,105 + -0.6
390,106 - +0.2
390,106 - -0.8
390,208 - +1.5
390,214 + +0.5
390,214 + -0.4
390,215 - +0.9
390,215 - +0.4
390,215 - +1.0
390,234 + +0.6
390,234 + +1.8
390,235 - +0.0
390,235 - +0.5
390,235 - +0.8
390,235 - -0.5
390,235 - +0.6
390,423 + -2.2
390,423 + -0.3
390,423 + +0.2
390,440 - +1.4
390,626 - Pf6N2E2_322 0.20 +0.9
391,086 + Pf6N2E2_322 0.66 +0.3
391,086 + Pf6N2E2_322 0.66 -0.6

Or see this region's nucleotide sequence