Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3062

Experiment: outer cut, LB soft agar motility assay

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPf6N2E2_3061 and Pf6N2E2_3062 are separated by 74 nucleotidesPf6N2E2_3062 and Pf6N2E2_3063 are separated by 67 nucleotides Pf6N2E2_3061 - Outer membrane protein assembly factor YaeT precursor, at 3,546,295 to 3,548,682 _3061 Pf6N2E2_3062 - Membrane-associated zinc metalloprotease, at 3,548,757 to 3,550,094 _3062 Pf6N2E2_3063 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase (EC 1.1.1.267), at 3,550,162 to 3,551,352 _3063 Position (kb) 3548 3549 3550 3551Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 3548.957 kb on - strand, within Pf6N2E2_3062at 3549.299 kb on + strand, within Pf6N2E2_3062at 3549.300 kb on - strand, within Pf6N2E2_3062at 3549.300 kb on - strand, within Pf6N2E2_3062at 3549.302 kb on + strand, within Pf6N2E2_3062at 3549.822 kb on - strand, within Pf6N2E2_3062at 3549.822 kb on - strand, within Pf6N2E2_3062at 3549.822 kb on - strand, within Pf6N2E2_3062at 3549.822 kb on - strand, within Pf6N2E2_3062at 3549.908 kb on - strand, within Pf6N2E2_3062at 3550.094 kb on + strandat 3550.095 kb on - strandat 3550.095 kb on - strandat 3550.095 kb on - strandat 3550.095 kb on - strandat 3550.095 kb on - strandat 3550.095 kb on - strandat 3550.097 kb on - strandat 3550.097 kb on - strandat 3550.152 kb on + strandat 3550.152 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
remove
3,548,957 - Pf6N2E2_3062 0.15 +4.4
3,549,299 + Pf6N2E2_3062 0.41 +3.3
3,549,300 - Pf6N2E2_3062 0.41 +1.9
3,549,300 - Pf6N2E2_3062 0.41 +3.2
3,549,302 + Pf6N2E2_3062 0.41 +3.9
3,549,822 - Pf6N2E2_3062 0.80 +5.8
3,549,822 - Pf6N2E2_3062 0.80 +1.3
3,549,822 - Pf6N2E2_3062 0.80 +1.7
3,549,822 - Pf6N2E2_3062 0.80 +1.9
3,549,908 - Pf6N2E2_3062 0.86 +2.1
3,550,094 + +4.0
3,550,095 - +0.7
3,550,095 - +1.7
3,550,095 - +3.0
3,550,095 - -0.8
3,550,095 - +2.3
3,550,095 - +2.0
3,550,097 - +0.5
3,550,097 - +2.8
3,550,152 + -0.6
3,550,152 + +1.4

Or see this region's nucleotide sequence