Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2616 and Pf6N2E2_2617 overlap by 4 nucleotides Pf6N2E2_2617 and Pf6N2E2_2618 overlap by 4 nucleotides Pf6N2E2_2618 and Pf6N2E2_2619 overlap by 10 nucleotides
Pf6N2E2_2616 - methyltransferase, putative, at 3,074,436 to 3,075,662
_2616
Pf6N2E2_2617 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 3,075,659 to 3,076,219
_2617
Pf6N2E2_2618 - Similar to CDP-glucose 4,6-dehydratase (EC 4.2.1.45), at 3,076,216 to 3,077,289
_2618
Pf6N2E2_2619 - Glucose-1-phosphate cytidylyltransferase (EC 2.7.7.33), at 3,077,280 to 3,078,038
_2619
Position (kb)
3076
3077
3078 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3075.409 kb on + strand, within Pf6N2E2_2616 at 3075.454 kb on + strand, within Pf6N2E2_2616 at 3075.455 kb on - strand, within Pf6N2E2_2616 at 3075.460 kb on + strand, within Pf6N2E2_2616 at 3075.460 kb on + strand, within Pf6N2E2_2616 at 3075.461 kb on - strand, within Pf6N2E2_2616 at 3075.461 kb on - strand, within Pf6N2E2_2616 at 3075.461 kb on - strand, within Pf6N2E2_2616 at 3075.461 kb on - strand, within Pf6N2E2_2616 at 3075.461 kb on - strand, within Pf6N2E2_2616 at 3075.493 kb on + strand, within Pf6N2E2_2616 at 3075.494 kb on - strand, within Pf6N2E2_2616 at 3075.494 kb on - strand, within Pf6N2E2_2616 at 3075.587 kb on - strand at 3075.587 kb on - strand at 3075.587 kb on - strand at 3075.609 kb on - strand at 3075.663 kb on - strand at 3075.724 kb on + strand, within Pf6N2E2_2617 at 3075.725 kb on - strand, within Pf6N2E2_2617 at 3075.799 kb on - strand, within Pf6N2E2_2617 at 3075.809 kb on - strand, within Pf6N2E2_2617 at 3075.816 kb on + strand, within Pf6N2E2_2617 at 3075.816 kb on + strand, within Pf6N2E2_2617 at 3075.817 kb on - strand, within Pf6N2E2_2617 at 3075.817 kb on - strand, within Pf6N2E2_2617 at 3075.947 kb on + strand, within Pf6N2E2_2617 at 3075.947 kb on + strand, within Pf6N2E2_2617 at 3075.947 kb on + strand, within Pf6N2E2_2617 at 3075.948 kb on - strand, within Pf6N2E2_2617 at 3076.233 kb on - strand at 3076.399 kb on + strand, within Pf6N2E2_2618 at 3076.399 kb on + strand, within Pf6N2E2_2618 at 3076.399 kb on + strand, within Pf6N2E2_2618 at 3076.399 kb on + strand, within Pf6N2E2_2618 at 3076.399 kb on + strand, within Pf6N2E2_2618 at 3076.400 kb on - strand, within Pf6N2E2_2618 at 3076.400 kb on - strand, within Pf6N2E2_2618 at 3076.400 kb on - strand, within Pf6N2E2_2618 at 3076.400 kb on - strand, within Pf6N2E2_2618 at 3076.400 kb on - strand, within Pf6N2E2_2618 at 3076.400 kb on - strand, within Pf6N2E2_2618 at 3076.539 kb on - strand, within Pf6N2E2_2618 at 3076.558 kb on + strand, within Pf6N2E2_2618 at 3076.559 kb on - strand, within Pf6N2E2_2618 at 3076.559 kb on - strand, within Pf6N2E2_2618 at 3076.559 kb on - strand, within Pf6N2E2_2618 at 3076.559 kb on - strand, within Pf6N2E2_2618 at 3076.618 kb on + strand, within Pf6N2E2_2618 at 3076.618 kb on + strand, within Pf6N2E2_2618 at 3076.618 kb on + strand, within Pf6N2E2_2618 at 3076.699 kb on + strand, within Pf6N2E2_2618 at 3076.776 kb on - strand, within Pf6N2E2_2618 at 3076.880 kb on + strand, within Pf6N2E2_2618 at 3076.880 kb on + strand, within Pf6N2E2_2618 at 3076.881 kb on - strand, within Pf6N2E2_2618 at 3076.881 kb on - strand, within Pf6N2E2_2618 at 3076.976 kb on + strand, within Pf6N2E2_2618 at 3076.976 kb on + strand, within Pf6N2E2_2618 at 3076.976 kb on + strand, within Pf6N2E2_2618 at 3076.977 kb on - strand, within Pf6N2E2_2618 at 3076.977 kb on - strand, within Pf6N2E2_2618 at 3076.977 kb on - strand, within Pf6N2E2_2618 at 3076.981 kb on - strand, within Pf6N2E2_2618 at 3077.236 kb on - strand at 3077.456 kb on + strand, within Pf6N2E2_2619 at 3077.456 kb on + strand, within Pf6N2E2_2619 at 3077.457 kb on - strand, within Pf6N2E2_2619 at 3077.457 kb on - strand, within Pf6N2E2_2619 at 3077.457 kb on - strand, within Pf6N2E2_2619 at 3077.457 kb on - strand, within Pf6N2E2_2619 at 3077.457 kb on - strand, within Pf6N2E2_2619 at 3077.459 kb on - strand, within Pf6N2E2_2619 at 3077.459 kb on - strand, within Pf6N2E2_2619 at 3077.459 kb on - strand, within Pf6N2E2_2619 at 3077.459 kb on - strand, within Pf6N2E2_2619 at 3077.481 kb on + strand, within Pf6N2E2_2619 at 3077.674 kb on + strand, within Pf6N2E2_2619 at 3077.674 kb on + strand, within Pf6N2E2_2619 at 3077.675 kb on - strand, within Pf6N2E2_2619 at 3077.788 kb on + strand, within Pf6N2E2_2619 at 3077.788 kb on + strand, within Pf6N2E2_2619 at 3077.788 kb on + strand, within Pf6N2E2_2619 at 3077.822 kb on + strand, within Pf6N2E2_2619 at 3077.823 kb on - strand, within Pf6N2E2_2619 at 3077.854 kb on + strand, within Pf6N2E2_2619 at 3077.854 kb on + strand, within Pf6N2E2_2619 at 3077.855 kb on - strand, within Pf6N2E2_2619 at 3077.881 kb on + strand, within Pf6N2E2_2619 at 3077.882 kb on - strand, within Pf6N2E2_2619 at 3077.891 kb on - strand, within Pf6N2E2_2619 at 3077.891 kb on - strand, within Pf6N2E2_2619 at 3077.891 kb on - strand, within Pf6N2E2_2619 at 3078.041 kb on + strand at 3078.042 kb on - strand at 3078.119 kb on - strand at 3078.170 kb on - strand at 3078.177 kb on + strand at 3078.178 kb on - strand at 3078.265 kb on - strand at 3078.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,075,409 + Pf6N2E2_2616 0.79 +1.4 3,075,454 + Pf6N2E2_2616 0.83 -0.0 3,075,455 - Pf6N2E2_2616 0.83 +0.7 3,075,460 + Pf6N2E2_2616 0.83 -0.1 3,075,460 + Pf6N2E2_2616 0.83 +2.0 3,075,461 - Pf6N2E2_2616 0.84 +0.1 3,075,461 - Pf6N2E2_2616 0.84 -0.4 3,075,461 - Pf6N2E2_2616 0.84 +0.1 3,075,461 - Pf6N2E2_2616 0.84 +0.0 3,075,461 - Pf6N2E2_2616 0.84 +1.0 3,075,493 + Pf6N2E2_2616 0.86 -0.8 3,075,494 - Pf6N2E2_2616 0.86 +0.5 3,075,494 - Pf6N2E2_2616 0.86 +1.2 3,075,587 - +1.1 3,075,587 - -1.2 3,075,587 - +0.0 3,075,609 - -0.1 3,075,663 - -0.2 3,075,724 + Pf6N2E2_2617 0.12 -1.1 3,075,725 - Pf6N2E2_2617 0.12 -2.2 3,075,799 - Pf6N2E2_2617 0.25 -1.3 3,075,809 - Pf6N2E2_2617 0.27 +1.3 3,075,816 + Pf6N2E2_2617 0.28 -0.8 3,075,816 + Pf6N2E2_2617 0.28 +0.3 3,075,817 - Pf6N2E2_2617 0.28 -0.6 3,075,817 - Pf6N2E2_2617 0.28 -0.8 3,075,947 + Pf6N2E2_2617 0.51 +1.5 3,075,947 + Pf6N2E2_2617 0.51 +0.2 3,075,947 + Pf6N2E2_2617 0.51 +1.0 3,075,948 - Pf6N2E2_2617 0.52 +0.4 3,076,233 - -0.7 3,076,399 + Pf6N2E2_2618 0.17 +0.5 3,076,399 + Pf6N2E2_2618 0.17 +1.7 3,076,399 + Pf6N2E2_2618 0.17 +2.1 3,076,399 + Pf6N2E2_2618 0.17 +2.5 3,076,399 + Pf6N2E2_2618 0.17 +0.3 3,076,400 - Pf6N2E2_2618 0.17 +1.2 3,076,400 - Pf6N2E2_2618 0.17 -0.6 3,076,400 - Pf6N2E2_2618 0.17 +0.2 3,076,400 - Pf6N2E2_2618 0.17 -1.1 3,076,400 - Pf6N2E2_2618 0.17 -1.6 3,076,400 - Pf6N2E2_2618 0.17 +0.2 3,076,539 - Pf6N2E2_2618 0.30 +0.1 3,076,558 + Pf6N2E2_2618 0.32 -1.4 3,076,559 - Pf6N2E2_2618 0.32 +1.9 3,076,559 - Pf6N2E2_2618 0.32 -0.6 3,076,559 - Pf6N2E2_2618 0.32 -0.6 3,076,559 - Pf6N2E2_2618 0.32 +0.8 3,076,618 + Pf6N2E2_2618 0.37 -0.2 3,076,618 + Pf6N2E2_2618 0.37 +1.5 3,076,618 + Pf6N2E2_2618 0.37 -3.1 3,076,699 + Pf6N2E2_2618 0.45 +0.7 3,076,776 - Pf6N2E2_2618 0.52 -0.8 3,076,880 + Pf6N2E2_2618 0.62 +0.6 3,076,880 + Pf6N2E2_2618 0.62 +0.7 3,076,881 - Pf6N2E2_2618 0.62 +0.4 3,076,881 - Pf6N2E2_2618 0.62 +0.3 3,076,976 + Pf6N2E2_2618 0.71 -0.7 3,076,976 + Pf6N2E2_2618 0.71 +0.7 3,076,976 + Pf6N2E2_2618 0.71 +1.4 3,076,977 - Pf6N2E2_2618 0.71 -0.0 3,076,977 - Pf6N2E2_2618 0.71 -1.1 3,076,977 - Pf6N2E2_2618 0.71 -2.5 3,076,981 - Pf6N2E2_2618 0.71 -0.0 3,077,236 - -0.4 3,077,456 + Pf6N2E2_2619 0.23 +1.3 3,077,456 + Pf6N2E2_2619 0.23 +1.9 3,077,457 - Pf6N2E2_2619 0.23 +1.0 3,077,457 - Pf6N2E2_2619 0.23 +0.0 3,077,457 - Pf6N2E2_2619 0.23 -0.3 3,077,457 - Pf6N2E2_2619 0.23 -0.2 3,077,457 - Pf6N2E2_2619 0.23 +0.5 3,077,459 - Pf6N2E2_2619 0.24 +0.0 3,077,459 - Pf6N2E2_2619 0.24 -0.9 3,077,459 - Pf6N2E2_2619 0.24 +1.1 3,077,459 - Pf6N2E2_2619 0.24 +0.9 3,077,481 + Pf6N2E2_2619 0.26 +1.2 3,077,674 + Pf6N2E2_2619 0.52 +0.4 3,077,674 + Pf6N2E2_2619 0.52 +1.6 3,077,675 - Pf6N2E2_2619 0.52 +1.9 3,077,788 + Pf6N2E2_2619 0.67 +1.3 3,077,788 + Pf6N2E2_2619 0.67 -0.3 3,077,788 + Pf6N2E2_2619 0.67 +0.6 3,077,822 + Pf6N2E2_2619 0.71 -1.5 3,077,823 - Pf6N2E2_2619 0.72 -1.3 3,077,854 + Pf6N2E2_2619 0.76 -1.6 3,077,854 + Pf6N2E2_2619 0.76 -0.3 3,077,855 - Pf6N2E2_2619 0.76 +2.2 3,077,881 + Pf6N2E2_2619 0.79 +1.0 3,077,882 - Pf6N2E2_2619 0.79 -1.7 3,077,891 - Pf6N2E2_2619 0.81 +2.2 3,077,891 - Pf6N2E2_2619 0.81 +0.3 3,077,891 - Pf6N2E2_2619 0.81 +1.0 3,078,041 + +1.1 3,078,042 - +0.0 3,078,119 - +0.5 3,078,170 - +0.2 3,078,177 + +1.1 3,078,178 - -0.1 3,078,265 - -0.6 3,078,282 - -1.4
Or see this region's nucleotide sequence