Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2592

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2590 and Pf6N2E2_2591 overlap by 11 nucleotidesPf6N2E2_2591 and Pf6N2E2_2592 are separated by 7 nucleotidesPf6N2E2_2592 and Pf6N2E2_2593 overlap by 8 nucleotides Pf6N2E2_2590 - Flagellum-specific ATP synthase FliI, at 3,046,787 to 3,048,145 _2590 Pf6N2E2_2591 - Flagellar assembly protein FliH, at 3,048,135 to 3,048,980 _2591 Pf6N2E2_2592 - Flagellar motor switch protein FliG, at 3,048,988 to 3,050,007 _2592 Pf6N2E2_2593 - Flagellar M-ring protein FliF, at 3,050,000 to 3,051,784 _2593 Position (kb) 3048 3049 3050 3051Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3048.103 kb on - strandat 3048.103 kb on - strandat 3048.180 kb on + strandat 3048.181 kb on - strandat 3048.469 kb on + strand, within Pf6N2E2_2591at 3048.469 kb on + strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.470 kb on - strand, within Pf6N2E2_2591at 3048.980 kb on + strandat 3048.980 kb on + strandat 3048.981 kb on - strandat 3048.981 kb on - strandat 3048.981 kb on - strandat 3048.981 kb on - strandat 3048.989 kb on + strandat 3048.989 kb on + strandat 3048.989 kb on + strandat 3048.989 kb on + strandat 3048.990 kb on - strandat 3048.990 kb on - strandat 3048.990 kb on - strandat 3049.207 kb on + strand, within Pf6N2E2_2592at 3049.760 kb on + strand, within Pf6N2E2_2592at 3049.760 kb on + strand, within Pf6N2E2_2592at 3049.846 kb on + strand, within Pf6N2E2_2592at 3049.846 kb on + strand, within Pf6N2E2_2592at 3049.846 kb on + strand, within Pf6N2E2_2592at 3049.847 kb on - strand, within Pf6N2E2_2592at 3049.847 kb on - strand, within Pf6N2E2_2592at 3049.847 kb on - strand, within Pf6N2E2_2592at 3049.847 kb on - strand, within Pf6N2E2_2592at 3049.847 kb on - strand, within Pf6N2E2_2592at 3050.094 kb on + strandat 3050.094 kb on + strandat 3050.095 kb on - strandat 3050.287 kb on + strand, within Pf6N2E2_2593at 3050.303 kb on + strand, within Pf6N2E2_2593at 3050.303 kb on + strand, within Pf6N2E2_2593at 3050.340 kb on + strand, within Pf6N2E2_2593at 3050.341 kb on - strand, within Pf6N2E2_2593

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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3,048,103 - -0.6
3,048,103 - -2.2
3,048,180 + -1.1
3,048,181 - -3.0
3,048,469 + Pf6N2E2_2591 0.39 -3.3
3,048,469 + Pf6N2E2_2591 0.39 -4.5
3,048,470 - Pf6N2E2_2591 0.40 -3.2
3,048,470 - Pf6N2E2_2591 0.40 -2.8
3,048,470 - Pf6N2E2_2591 0.40 -1.8
3,048,470 - Pf6N2E2_2591 0.40 -3.2
3,048,470 - Pf6N2E2_2591 0.40 -1.7
3,048,470 - Pf6N2E2_2591 0.40 -1.0
3,048,470 - Pf6N2E2_2591 0.40 -2.1
3,048,980 + -1.9
3,048,980 + -1.5
3,048,981 - -2.1
3,048,981 - -3.1
3,048,981 - -1.7
3,048,981 - -2.2
3,048,989 + -2.6
3,048,989 + -2.5
3,048,989 + -1.7
3,048,989 + -1.3
3,048,990 - -0.9
3,048,990 - +0.2
3,048,990 - -1.6
3,049,207 + Pf6N2E2_2592 0.21 -1.9
3,049,760 + Pf6N2E2_2592 0.76 -2.3
3,049,760 + Pf6N2E2_2592 0.76 +0.1
3,049,846 + Pf6N2E2_2592 0.84 -1.4
3,049,846 + Pf6N2E2_2592 0.84 -2.8
3,049,846 + Pf6N2E2_2592 0.84 -2.4
3,049,847 - Pf6N2E2_2592 0.84 -1.8
3,049,847 - Pf6N2E2_2592 0.84 -3.5
3,049,847 - Pf6N2E2_2592 0.84 -3.2
3,049,847 - Pf6N2E2_2592 0.84 -3.4
3,049,847 - Pf6N2E2_2592 0.84 -3.3
3,050,094 + -3.2
3,050,094 + -1.3
3,050,095 - -3.0
3,050,287 + Pf6N2E2_2593 0.16 -1.6
3,050,303 + Pf6N2E2_2593 0.17 -3.5
3,050,303 + Pf6N2E2_2593 0.17 -1.6
3,050,340 + Pf6N2E2_2593 0.19 -2.1
3,050,341 - Pf6N2E2_2593 0.19 -0.1

Or see this region's nucleotide sequence