Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2579

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2577 and Pf6N2E2_2578 are separated by 3 nucleotidesPf6N2E2_2578 and Pf6N2E2_2579 are separated by 4 nucleotidesPf6N2E2_2579 and Pf6N2E2_2580 are separated by 23 nucleotidesPf6N2E2_2580 and Pf6N2E2_2581 overlap by 1 nucleotides Pf6N2E2_2577 - Flagellar biosynthesis protein FlhB, at 3,036,721 to 3,037,857 _2577 Pf6N2E2_2578 - Flagellar biosynthesis protein FliR, at 3,037,861 to 3,038,643 _2578 Pf6N2E2_2579 - Flagellar biosynthesis protein FliQ, at 3,038,648 to 3,038,917 _2579 Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690 _2580 Pf6N2E2_2581 - Flagellar biosynthesis protein FliQ, at 3,039,690 to 3,040,127 _2581 Position (kb) 3038 3039Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.709 kb on + strand, within Pf6N2E2_2577at 3037.710 kb on - strand, within Pf6N2E2_2577at 3037.710 kb on - strand, within Pf6N2E2_2577at 3037.710 kb on - strand, within Pf6N2E2_2577at 3037.885 kb on + strandat 3037.886 kb on - strandat 3038.077 kb on + strand, within Pf6N2E2_2578at 3038.286 kb on + strand, within Pf6N2E2_2578at 3038.504 kb on + strand, within Pf6N2E2_2578at 3038.607 kb on + strandat 3038.669 kb on - strandat 3038.669 kb on - strandat 3038.677 kb on - strand, within Pf6N2E2_2579at 3038.677 kb on - strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.691 kb on + strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.692 kb on - strand, within Pf6N2E2_2579at 3038.709 kb on - strand, within Pf6N2E2_2579at 3038.861 kb on + strand, within Pf6N2E2_2579at 3038.861 kb on + strand, within Pf6N2E2_2579at 3038.862 kb on - strand, within Pf6N2E2_2579at 3038.903 kb on + strandat 3038.903 kb on + strandat 3038.904 kb on - strandat 3038.917 kb on + strandat 3038.942 kb on + strandat 3038.943 kb on - strandat 3039.207 kb on + strand, within Pf6N2E2_2580at 3039.335 kb on + strand, within Pf6N2E2_2580at 3039.335 kb on + strand, within Pf6N2E2_2580at 3039.336 kb on - strand, within Pf6N2E2_2580at 3039.336 kb on - strand, within Pf6N2E2_2580at 3039.569 kb on + strand, within Pf6N2E2_2580at 3039.569 kb on + strand, within Pf6N2E2_2580at 3039.692 kb on - strandat 3039.692 kb on - strandat 3039.855 kb on + strand, within Pf6N2E2_2581

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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3,037,709 + Pf6N2E2_2577 0.87 -0.9
3,037,709 + Pf6N2E2_2577 0.87 -4.0
3,037,709 + Pf6N2E2_2577 0.87 -1.8
3,037,709 + Pf6N2E2_2577 0.87 -3.0
3,037,709 + Pf6N2E2_2577 0.87 -2.2
3,037,709 + Pf6N2E2_2577 0.87 -0.1
3,037,709 + Pf6N2E2_2577 0.87 -3.9
3,037,710 - Pf6N2E2_2577 0.87 -3.7
3,037,710 - Pf6N2E2_2577 0.87 -1.6
3,037,710 - Pf6N2E2_2577 0.87 -2.7
3,037,885 + -2.8
3,037,886 - -0.7
3,038,077 + Pf6N2E2_2578 0.28 -2.3
3,038,286 + Pf6N2E2_2578 0.54 -4.2
3,038,504 + Pf6N2E2_2578 0.82 -1.6
3,038,607 + -3.6
3,038,669 - -2.3
3,038,669 - -3.4
3,038,677 - Pf6N2E2_2579 0.11 -2.2
3,038,677 - Pf6N2E2_2579 0.11 -1.3
3,038,691 + Pf6N2E2_2579 0.16 +0.1
3,038,691 + Pf6N2E2_2579 0.16 -1.7
3,038,691 + Pf6N2E2_2579 0.16 -1.8
3,038,691 + Pf6N2E2_2579 0.16 -2.5
3,038,692 - Pf6N2E2_2579 0.16 -2.2
3,038,692 - Pf6N2E2_2579 0.16 -2.0
3,038,692 - Pf6N2E2_2579 0.16 -3.0
3,038,692 - Pf6N2E2_2579 0.16 -2.9
3,038,709 - Pf6N2E2_2579 0.23 -2.1
3,038,861 + Pf6N2E2_2579 0.79 -1.3
3,038,861 + Pf6N2E2_2579 0.79 -0.8
3,038,862 - Pf6N2E2_2579 0.79 -2.8
3,038,903 + -0.8
3,038,903 + -2.6
3,038,904 - -1.3
3,038,917 + -2.1
3,038,942 + -0.9
3,038,943 - -0.8
3,039,207 + Pf6N2E2_2580 0.35 -2.9
3,039,335 + Pf6N2E2_2580 0.53 -2.0
3,039,335 + Pf6N2E2_2580 0.53 -2.1
3,039,336 - Pf6N2E2_2580 0.53 -1.4
3,039,336 - Pf6N2E2_2580 0.53 -1.6
3,039,569 + Pf6N2E2_2580 0.84 -2.0
3,039,569 + Pf6N2E2_2580 0.84 -2.1
3,039,692 - -2.8
3,039,692 - -2.6
3,039,855 + Pf6N2E2_2581 0.38 -1.4

Or see this region's nucleotide sequence