Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2570 and Pf6N2E2_2571 are separated by 30 nucleotides Pf6N2E2_2571 and Pf6N2E2_2572 are separated by 36 nucleotides Pf6N2E2_2572 and Pf6N2E2_2573 are separated by 155 nucleotides
Pf6N2E2_2570 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 3,027,571 to 3,029,829
_2570
Pf6N2E2_2571 - Chemotaxis response - phosphatase CheZ, at 3,029,860 to 3,030,627
_2571
Pf6N2E2_2572 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 3,030,664 to 3,031,047
_2572
Pf6N2E2_2573 - RNA polymerase sigma factor for flagellar operon, at 3,031,203 to 3,031,946
_2573
Position (kb)
3029
3030
3031 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3029.004 kb on + strand, within Pf6N2E2_2570 at 3029.329 kb on - strand, within Pf6N2E2_2570 at 3029.677 kb on + strand at 3029.677 kb on + strand at 3029.677 kb on + strand at 3029.678 kb on - strand at 3029.678 kb on - strand at 3029.678 kb on - strand at 3029.767 kb on + strand at 3029.767 kb on + strand at 3029.767 kb on + strand at 3029.767 kb on + strand at 3029.768 kb on - strand at 3029.843 kb on + strand at 3029.843 kb on + strand at 3029.843 kb on + strand at 3029.844 kb on - strand at 3029.854 kb on + strand at 3029.855 kb on - strand at 3029.910 kb on + strand at 3029.910 kb on + strand at 3029.910 kb on + strand at 3029.910 kb on + strand at 3029.911 kb on - strand at 3029.911 kb on - strand at 3029.911 kb on - strand at 3029.911 kb on - strand at 3029.911 kb on - strand at 3029.926 kb on - strand at 3030.125 kb on + strand, within Pf6N2E2_2571 at 3030.125 kb on + strand, within Pf6N2E2_2571 at 3030.126 kb on - strand, within Pf6N2E2_2571 at 3030.126 kb on - strand, within Pf6N2E2_2571 at 3030.126 kb on - strand, within Pf6N2E2_2571 at 3030.377 kb on + strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.378 kb on - strand, within Pf6N2E2_2571 at 3030.645 kb on + strand at 3030.645 kb on + strand at 3030.645 kb on + strand at 3030.645 kb on + strand at 3030.646 kb on - strand at 3030.646 kb on - strand at 3030.734 kb on + strand, within Pf6N2E2_2572 at 3030.735 kb on - strand, within Pf6N2E2_2572 at 3030.743 kb on - strand, within Pf6N2E2_2572 at 3030.988 kb on + strand, within Pf6N2E2_2572 at 3031.067 kb on + strand at 3031.067 kb on + strand at 3031.068 kb on - strand at 3031.154 kb on + strand at 3031.155 kb on - strand at 3031.155 kb on - strand at 3031.261 kb on - strand at 3031.261 kb on - strand at 3031.334 kb on - strand, within Pf6N2E2_2573 at 3031.334 kb on - strand, within Pf6N2E2_2573 at 3031.334 kb on - strand, within Pf6N2E2_2573 at 3031.520 kb on + strand, within Pf6N2E2_2573 at 3031.520 kb on + strand, within Pf6N2E2_2573 at 3031.521 kb on - strand, within Pf6N2E2_2573 at 3031.540 kb on + strand, within Pf6N2E2_2573 at 3031.586 kb on + strand, within Pf6N2E2_2573 at 3031.604 kb on + strand, within Pf6N2E2_2573
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,029,004 + Pf6N2E2_2570 0.63 -2.0 3,029,329 - Pf6N2E2_2570 0.78 +0.5 3,029,677 + -2.1 3,029,677 + -2.4 3,029,677 + -2.3 3,029,678 - -2.2 3,029,678 - -3.1 3,029,678 - -2.7 3,029,767 + -3.6 3,029,767 + -1.4 3,029,767 + -3.1 3,029,767 + -1.7 3,029,768 - -2.1 3,029,843 + -3.1 3,029,843 + -1.0 3,029,843 + -0.6 3,029,844 - -1.1 3,029,854 + -3.1 3,029,855 - -1.6 3,029,910 + -3.8 3,029,910 + -1.7 3,029,910 + -1.5 3,029,910 + -1.6 3,029,911 - +0.2 3,029,911 - -1.2 3,029,911 - -1.8 3,029,911 - -0.4 3,029,911 - -0.6 3,029,926 - -0.8 3,030,125 + Pf6N2E2_2571 0.35 -1.3 3,030,125 + Pf6N2E2_2571 0.35 -1.8 3,030,126 - Pf6N2E2_2571 0.35 -1.0 3,030,126 - Pf6N2E2_2571 0.35 +0.7 3,030,126 - Pf6N2E2_2571 0.35 +0.1 3,030,377 + Pf6N2E2_2571 0.67 -2.2 3,030,378 - Pf6N2E2_2571 0.67 -1.7 3,030,378 - Pf6N2E2_2571 0.67 -2.3 3,030,378 - Pf6N2E2_2571 0.67 -3.0 3,030,378 - Pf6N2E2_2571 0.67 -1.0 3,030,378 - Pf6N2E2_2571 0.67 -2.2 3,030,378 - Pf6N2E2_2571 0.67 -2.9 3,030,645 + -0.6 3,030,645 + -1.3 3,030,645 + -2.6 3,030,645 + -2.4 3,030,646 - -2.4 3,030,646 - -0.8 3,030,734 + Pf6N2E2_2572 0.18 -0.6 3,030,735 - Pf6N2E2_2572 0.18 -2.9 3,030,743 - Pf6N2E2_2572 0.21 -3.4 3,030,988 + Pf6N2E2_2572 0.84 -1.3 3,031,067 + -1.6 3,031,067 + -1.8 3,031,068 - -2.4 3,031,154 + -1.1 3,031,155 - +4.6 3,031,155 - +2.8 3,031,261 - -3.6 3,031,261 - -1.1 3,031,334 - Pf6N2E2_2573 0.18 -3.1 3,031,334 - Pf6N2E2_2573 0.18 -2.1 3,031,334 - Pf6N2E2_2573 0.18 -2.7 3,031,520 + Pf6N2E2_2573 0.43 -3.1 3,031,520 + Pf6N2E2_2573 0.43 -1.0 3,031,521 - Pf6N2E2_2573 0.43 -2.9 3,031,540 + Pf6N2E2_2573 0.45 -2.3 3,031,586 + Pf6N2E2_2573 0.51 +0.9 3,031,604 + Pf6N2E2_2573 0.54 -3.6
Or see this region's nucleotide sequence