Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2483 and Pf6N2E2_2484 are separated by 153 nucleotides Pf6N2E2_2484 and Pf6N2E2_2485 are separated by 258 nucleotides Pf6N2E2_2485 and Pf6N2E2_2486 are separated by 27 nucleotides
Pf6N2E2_2483 - Deoxyribonuclease TatD, at 2,929,438 to 2,930,247
_2483
Pf6N2E2_2484 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 2,930,401 to 2,931,882
_2484
Pf6N2E2_2485 - Phage terminase, small subunit, at 2,932,141 to 2,932,515
_2485
Pf6N2E2_2486 - TesB-like acyl-CoA thioesterase 1, at 2,932,543 to 2,933,340
_2486
Position (kb)
2930
2931
2932 Strain fitness (log2 ratio)
-2
-1
0
1 at 2929.405 kb on + strand at 2929.432 kb on - strand at 2929.516 kb on + strand at 2929.946 kb on - strand, within Pf6N2E2_2483 at 2930.129 kb on - strand, within Pf6N2E2_2483 at 2930.153 kb on + strand, within Pf6N2E2_2483 at 2930.154 kb on - strand, within Pf6N2E2_2483 at 2930.154 kb on - strand, within Pf6N2E2_2483 at 2930.245 kb on + strand at 2930.272 kb on + strand at 2930.275 kb on + strand at 2930.276 kb on - strand at 2930.276 kb on - strand at 2930.276 kb on - strand at 2930.416 kb on - strand at 2930.421 kb on + strand at 2930.421 kb on + strand at 2930.421 kb on + strand at 2930.421 kb on + strand at 2930.421 kb on + strand at 2930.421 kb on + strand at 2930.422 kb on - strand at 2930.422 kb on - strand at 2930.422 kb on - strand at 2930.434 kb on + strand at 2930.491 kb on + strand at 2930.492 kb on - strand at 2930.605 kb on + strand, within Pf6N2E2_2484 at 2930.605 kb on + strand, within Pf6N2E2_2484 at 2930.606 kb on - strand, within Pf6N2E2_2484 at 2930.606 kb on - strand, within Pf6N2E2_2484 at 2930.606 kb on - strand, within Pf6N2E2_2484 at 2930.606 kb on - strand, within Pf6N2E2_2484 at 2930.894 kb on + strand, within Pf6N2E2_2484 at 2930.895 kb on - strand, within Pf6N2E2_2484 at 2930.916 kb on + strand, within Pf6N2E2_2484 at 2930.916 kb on + strand, within Pf6N2E2_2484 at 2930.916 kb on + strand, within Pf6N2E2_2484 at 2930.916 kb on + strand, within Pf6N2E2_2484 at 2930.917 kb on - strand, within Pf6N2E2_2484 at 2930.917 kb on - strand, within Pf6N2E2_2484 at 2930.917 kb on - strand, within Pf6N2E2_2484 at 2930.917 kb on - strand, within Pf6N2E2_2484 at 2931.194 kb on - strand, within Pf6N2E2_2484 at 2931.765 kb on + strand at 2931.765 kb on + strand at 2931.766 kb on - strand at 2931.880 kb on + strand at 2932.015 kb on + strand at 2932.015 kb on + strand at 2932.015 kb on + strand at 2932.015 kb on + strand at 2932.015 kb on + strand at 2932.015 kb on + strand at 2932.016 kb on - strand at 2932.044 kb on + strand at 2932.045 kb on - strand at 2932.055 kb on - strand at 2932.134 kb on + strand at 2932.134 kb on + strand at 2932.134 kb on + strand at 2932.134 kb on + strand at 2932.187 kb on - strand, within Pf6N2E2_2485 at 2932.658 kb on + strand, within Pf6N2E2_2486 at 2932.658 kb on + strand, within Pf6N2E2_2486 at 2932.658 kb on + strand, within Pf6N2E2_2486
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,929,405 + +0.8 2,929,432 - +0.5 2,929,516 + -1.6 2,929,946 - Pf6N2E2_2483 0.63 +1.5 2,930,129 - Pf6N2E2_2483 0.85 -1.1 2,930,153 + Pf6N2E2_2483 0.88 +0.1 2,930,154 - Pf6N2E2_2483 0.88 -0.5 2,930,154 - Pf6N2E2_2483 0.88 +1.1 2,930,245 + +0.3 2,930,272 + -0.5 2,930,275 + -1.9 2,930,276 - -0.7 2,930,276 - -0.7 2,930,276 - -0.3 2,930,416 - +0.4 2,930,421 + +0.6 2,930,421 + +0.8 2,930,421 + -1.3 2,930,421 + +0.0 2,930,421 + -1.3 2,930,421 + -1.5 2,930,422 - +0.2 2,930,422 - -1.6 2,930,422 - +0.4 2,930,434 + -0.7 2,930,491 + -1.2 2,930,492 - -1.8 2,930,605 + Pf6N2E2_2484 0.14 -0.8 2,930,605 + Pf6N2E2_2484 0.14 -0.4 2,930,606 - Pf6N2E2_2484 0.14 +0.3 2,930,606 - Pf6N2E2_2484 0.14 +0.7 2,930,606 - Pf6N2E2_2484 0.14 -0.9 2,930,606 - Pf6N2E2_2484 0.14 -1.8 2,930,894 + Pf6N2E2_2484 0.33 -0.6 2,930,895 - Pf6N2E2_2484 0.33 -0.5 2,930,916 + Pf6N2E2_2484 0.35 -1.0 2,930,916 + Pf6N2E2_2484 0.35 +0.4 2,930,916 + Pf6N2E2_2484 0.35 -1.9 2,930,916 + Pf6N2E2_2484 0.35 -1.9 2,930,917 - Pf6N2E2_2484 0.35 -1.0 2,930,917 - Pf6N2E2_2484 0.35 -0.6 2,930,917 - Pf6N2E2_2484 0.35 -1.5 2,930,917 - Pf6N2E2_2484 0.35 -1.7 2,931,194 - Pf6N2E2_2484 0.54 -1.2 2,931,765 + -0.6 2,931,765 + -1.2 2,931,766 - -2.3 2,931,880 + +0.9 2,932,015 + -0.9 2,932,015 + +0.6 2,932,015 + -1.8 2,932,015 + -2.6 2,932,015 + -0.8 2,932,015 + +0.1 2,932,016 - +1.5 2,932,044 + +0.8 2,932,045 - -0.8 2,932,055 - -0.1 2,932,134 + -0.0 2,932,134 + -2.3 2,932,134 + -2.4 2,932,134 + +1.7 2,932,187 - Pf6N2E2_2485 0.12 +1.2 2,932,658 + Pf6N2E2_2486 0.14 +0.3 2,932,658 + Pf6N2E2_2486 0.14 +0.9 2,932,658 + Pf6N2E2_2486 0.14 -0.7
Or see this region's nucleotide sequence