Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1765 and Pf6N2E2_1766 are separated by 96 nucleotides Pf6N2E2_1766 and Pf6N2E2_1767 are separated by 495 nucleotides
Pf6N2E2_1765 - hypothetical protein, at 2,085,787 to 2,087,091
_1765
Pf6N2E2_1766 - Outer membrane transporter, OMPP1/FadL/TodX family, at 2,087,188 to 2,088,243
_1766
Pf6N2E2_1767 - Transcriptional regulator, AraC family, at 2,088,739 to 2,089,500
_1767
Position (kb)
2087
2088
2089 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2086.210 kb on + strand, within Pf6N2E2_1765 at 2086.236 kb on + strand, within Pf6N2E2_1765 at 2086.236 kb on + strand, within Pf6N2E2_1765 at 2086.237 kb on - strand, within Pf6N2E2_1765 at 2086.312 kb on + strand, within Pf6N2E2_1765 at 2086.346 kb on + strand, within Pf6N2E2_1765 at 2086.347 kb on - strand, within Pf6N2E2_1765 at 2086.571 kb on + strand, within Pf6N2E2_1765 at 2086.579 kb on + strand, within Pf6N2E2_1765 at 2086.628 kb on - strand, within Pf6N2E2_1765 at 2086.637 kb on - strand, within Pf6N2E2_1765 at 2086.637 kb on - strand, within Pf6N2E2_1765 at 2086.639 kb on - strand, within Pf6N2E2_1765 at 2086.831 kb on + strand, within Pf6N2E2_1765 at 2086.832 kb on - strand, within Pf6N2E2_1765 at 2086.925 kb on - strand, within Pf6N2E2_1765 at 2086.925 kb on - strand, within Pf6N2E2_1765 at 2087.138 kb on + strand at 2087.184 kb on - strand at 2087.302 kb on + strand, within Pf6N2E2_1766 at 2087.302 kb on + strand, within Pf6N2E2_1766 at 2087.464 kb on + strand, within Pf6N2E2_1766 at 2087.464 kb on + strand, within Pf6N2E2_1766 at 2087.479 kb on + strand, within Pf6N2E2_1766 at 2087.480 kb on - strand, within Pf6N2E2_1766 at 2087.518 kb on + strand, within Pf6N2E2_1766 at 2087.521 kb on - strand, within Pf6N2E2_1766 at 2087.521 kb on - strand, within Pf6N2E2_1766 at 2087.578 kb on + strand, within Pf6N2E2_1766 at 2087.579 kb on - strand, within Pf6N2E2_1766 at 2087.647 kb on + strand, within Pf6N2E2_1766 at 2087.648 kb on - strand, within Pf6N2E2_1766 at 2087.702 kb on - strand, within Pf6N2E2_1766 at 2087.738 kb on - strand, within Pf6N2E2_1766 at 2087.973 kb on + strand, within Pf6N2E2_1766 at 2087.973 kb on + strand, within Pf6N2E2_1766 at 2087.973 kb on + strand, within Pf6N2E2_1766 at 2087.974 kb on - strand, within Pf6N2E2_1766 at 2088.031 kb on + strand, within Pf6N2E2_1766 at 2088.148 kb on + strand at 2088.148 kb on + strand at 2088.148 kb on + strand at 2088.148 kb on + strand at 2088.149 kb on - strand at 2088.153 kb on + strand at 2088.179 kb on + strand at 2088.234 kb on + strand at 2088.234 kb on + strand at 2088.299 kb on + strand at 2088.345 kb on + strand at 2088.345 kb on + strand at 2088.426 kb on + strand at 2088.512 kb on - strand at 2088.550 kb on + strand at 2088.551 kb on - strand at 2088.551 kb on - strand at 2088.582 kb on + strand at 2088.583 kb on - strand at 2088.583 kb on - strand at 2088.583 kb on - strand at 2088.601 kb on + strand at 2088.634 kb on + strand at 2088.812 kb on - strand at 2088.812 kb on - strand at 2088.880 kb on + strand, within Pf6N2E2_1767 at 2088.881 kb on - strand, within Pf6N2E2_1767 at 2088.916 kb on + strand, within Pf6N2E2_1767 at 2088.917 kb on - strand, within Pf6N2E2_1767 at 2088.917 kb on - strand, within Pf6N2E2_1767 at 2089.057 kb on - strand, within Pf6N2E2_1767 at 2089.057 kb on - strand, within Pf6N2E2_1767 at 2089.058 kb on + strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.125 kb on + strand, within Pf6N2E2_1767 at 2089.125 kb on + strand, within Pf6N2E2_1767 at 2089.126 kb on - strand, within Pf6N2E2_1767 at 2089.180 kb on + strand, within Pf6N2E2_1767
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,086,210 + Pf6N2E2_1765 0.32 +1.6 2,086,236 + Pf6N2E2_1765 0.34 -0.6 2,086,236 + Pf6N2E2_1765 0.34 +0.9 2,086,237 - Pf6N2E2_1765 0.34 -1.1 2,086,312 + Pf6N2E2_1765 0.40 +0.9 2,086,346 + Pf6N2E2_1765 0.43 +1.2 2,086,347 - Pf6N2E2_1765 0.43 +1.0 2,086,571 + Pf6N2E2_1765 0.60 +0.2 2,086,579 + Pf6N2E2_1765 0.61 +0.6 2,086,628 - Pf6N2E2_1765 0.64 -1.5 2,086,637 - Pf6N2E2_1765 0.65 -1.1 2,086,637 - Pf6N2E2_1765 0.65 +0.4 2,086,639 - Pf6N2E2_1765 0.65 +0.3 2,086,831 + Pf6N2E2_1765 0.80 -1.1 2,086,832 - Pf6N2E2_1765 0.80 +1.0 2,086,925 - Pf6N2E2_1765 0.87 +0.2 2,086,925 - Pf6N2E2_1765 0.87 +1.0 2,087,138 + +1.5 2,087,184 - -0.5 2,087,302 + Pf6N2E2_1766 0.11 -1.5 2,087,302 + Pf6N2E2_1766 0.11 +0.0 2,087,464 + Pf6N2E2_1766 0.26 -0.2 2,087,464 + Pf6N2E2_1766 0.26 -0.0 2,087,479 + Pf6N2E2_1766 0.28 +0.6 2,087,480 - Pf6N2E2_1766 0.28 -0.2 2,087,518 + Pf6N2E2_1766 0.31 +0.2 2,087,521 - Pf6N2E2_1766 0.32 -1.4 2,087,521 - Pf6N2E2_1766 0.32 -2.6 2,087,578 + Pf6N2E2_1766 0.37 -3.3 2,087,579 - Pf6N2E2_1766 0.37 +0.0 2,087,647 + Pf6N2E2_1766 0.43 +0.0 2,087,648 - Pf6N2E2_1766 0.44 -0.9 2,087,702 - Pf6N2E2_1766 0.49 -2.2 2,087,738 - Pf6N2E2_1766 0.52 -0.6 2,087,973 + Pf6N2E2_1766 0.74 -0.3 2,087,973 + Pf6N2E2_1766 0.74 -0.5 2,087,973 + Pf6N2E2_1766 0.74 +0.8 2,087,974 - Pf6N2E2_1766 0.74 -1.0 2,088,031 + Pf6N2E2_1766 0.80 +0.0 2,088,148 + +0.1 2,088,148 + +1.0 2,088,148 + -0.9 2,088,148 + -1.8 2,088,149 - +1.2 2,088,153 + -1.6 2,088,179 + +0.6 2,088,234 + +0.2 2,088,234 + -1.5 2,088,299 + +0.2 2,088,345 + +1.6 2,088,345 + +0.0 2,088,426 + +1.2 2,088,512 - +0.2 2,088,550 + +0.8 2,088,551 - +0.9 2,088,551 - -0.8 2,088,582 + +1.2 2,088,583 - +2.3 2,088,583 - -0.7 2,088,583 - +0.6 2,088,601 + +0.3 2,088,634 + +0.4 2,088,812 - +1.0 2,088,812 - +1.4 2,088,880 + Pf6N2E2_1767 0.19 -2.0 2,088,881 - Pf6N2E2_1767 0.19 +0.6 2,088,916 + Pf6N2E2_1767 0.23 +0.4 2,088,917 - Pf6N2E2_1767 0.23 +0.9 2,088,917 - Pf6N2E2_1767 0.23 +0.3 2,089,057 - Pf6N2E2_1767 0.42 -0.9 2,089,057 - Pf6N2E2_1767 0.42 -1.5 2,089,058 + Pf6N2E2_1767 0.42 +1.0 2,089,067 - Pf6N2E2_1767 0.43 +1.1 2,089,067 - Pf6N2E2_1767 0.43 +0.3 2,089,067 - Pf6N2E2_1767 0.43 +1.5 2,089,067 - Pf6N2E2_1767 0.43 +1.3 2,089,125 + Pf6N2E2_1767 0.51 +0.2 2,089,125 + Pf6N2E2_1767 0.51 -1.3 2,089,126 - Pf6N2E2_1767 0.51 +0.2 2,089,180 + Pf6N2E2_1767 0.58 -0.8
Or see this region's nucleotide sequence